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Selective cytokine production by epithelial cells following exposure to Escherichia coli

Agace, W. LU ; Hedges, S. ; Andersson, U. LU ; Andersson, J. ; Ceska, M. and Svanborg, C. LU (1993) In Infection and Immunity 61(2). p.602-609
Abstract

This study compared the repertoire of cytokines produced by epithelial cell lines and human peripheral blood monocytes in response to Escherichia coli. The A-498 and J82 urinary tract epithelial cell lines and human peripheral blood monocytes were exposed to E. coli Hu734. The cytokine content of single cells was detected by indirect immunofluorescence using monoclonal antibodies to interleukin-1α (IL-1α), IL-1β, tumor necrosis factor alpha (TNF-α), TNF-β, IL-6, IL-8, and granulocyte macrophage- colony-stimulating factor, and the number of positive cells was used to quantitate the response. The J82 bladder cell line stained positive for IL- 6, IL-8, and IL-1α. The IL-8 and IL-6 response peaked at 2 h, while the number of IL-1α-positive... (More)

This study compared the repertoire of cytokines produced by epithelial cell lines and human peripheral blood monocytes in response to Escherichia coli. The A-498 and J82 urinary tract epithelial cell lines and human peripheral blood monocytes were exposed to E. coli Hu734. The cytokine content of single cells was detected by indirect immunofluorescence using monoclonal antibodies to interleukin-1α (IL-1α), IL-1β, tumor necrosis factor alpha (TNF-α), TNF-β, IL-6, IL-8, and granulocyte macrophage- colony-stimulating factor, and the number of positive cells was used to quantitate the response. The J82 bladder cell line stained positive for IL- 6, IL-8, and IL-1α. The IL-8 and IL-6 response peaked at 2 h, while the number of IL-1α-positive cells reached a peak 6 h after E. coli stimulation. The A-498 kidney cell line stained for IL-8 with a peak at 2 h and IL-6 with a peak at 6 h after E. coli stimulation. Peripheral blood monocytes stained for the cytokines IL-1α, IL-1β, IL-8, IL-6, and TNF-α but not for TNF-β and granulocyte macrophage-colony-stimulating factor after stimulation with E. coli. The results demonstrated that bacteria activated a cytokine response in the epithelial cell lines and monocytes. The epithelial cell lines had a more limited cytokine response profile than circulating monocytes, which may serve to limit the consequences of microbial exposure at the mucosal surface and help maintain the integrity of other tissue compartments.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Infection and Immunity
volume
61
issue
2
pages
602 - 609
publisher
American Society for Microbiology
external identifiers
  • pmid:8423089
  • scopus:0027510075
ISSN
0019-9567
language
English
LU publication?
yes
id
42a3e519-d8f2-4144-ba7f-46ba1b02032b
date added to LUP
2019-05-30 14:00:49
date last changed
2020-08-02 06:48:32
@article{42a3e519-d8f2-4144-ba7f-46ba1b02032b,
  abstract     = {<p>This study compared the repertoire of cytokines produced by epithelial cell lines and human peripheral blood monocytes in response to Escherichia coli. The A-498 and J82 urinary tract epithelial cell lines and human peripheral blood monocytes were exposed to E. coli Hu734. The cytokine content of single cells was detected by indirect immunofluorescence using monoclonal antibodies to interleukin-1α (IL-1α), IL-1β, tumor necrosis factor alpha (TNF-α), TNF-β, IL-6, IL-8, and granulocyte macrophage- colony-stimulating factor, and the number of positive cells was used to quantitate the response. The J82 bladder cell line stained positive for IL- 6, IL-8, and IL-1α. The IL-8 and IL-6 response peaked at 2 h, while the number of IL-1α-positive cells reached a peak 6 h after E. coli stimulation. The A-498 kidney cell line stained for IL-8 with a peak at 2 h and IL-6 with a peak at 6 h after E. coli stimulation. Peripheral blood monocytes stained for the cytokines IL-1α, IL-1β, IL-8, IL-6, and TNF-α but not for TNF-β and granulocyte macrophage-colony-stimulating factor after stimulation with E. coli. The results demonstrated that bacteria activated a cytokine response in the epithelial cell lines and monocytes. The epithelial cell lines had a more limited cytokine response profile than circulating monocytes, which may serve to limit the consequences of microbial exposure at the mucosal surface and help maintain the integrity of other tissue compartments.</p>},
  author       = {Agace, W. and Hedges, S. and Andersson, U. and Andersson, J. and Ceska, M. and Svanborg, C.},
  issn         = {0019-9567},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {602--609},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {Selective cytokine production by epithelial cells following exposure to Escherichia coli},
  volume       = {61},
  year         = {1993},
}