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Plant stem cell maintenance involves direct transcriptional repression of differentiation program

Yadav, Ram Kishor; Perales, Mariano; Gruel, Jeremy LU ; Ohno, Carolyn; Heisler, Marcus; Girke, Thomas; Jönsson, Henrik LU and Reddy, G. Venugopala (2013) In Molecular Systems Biology 9. p.654-654
Abstract
In animal systems, master regulatory transcription factors (TFs) mediate stem cell maintenance through a direct transcriptional repression of differentiation promoting TFs. Whether similar mechanisms operate in plants is not known. In plants, shoot apical meristems serve as reservoirs of stem cells that provide cells for all above ground organs. WUSCHEL, a homeodomain TF produced in cells of the niche, migrates into adjacent cells where it specifies stem cells. Through high-resolution genomic analysis, we show that WUSCHEL represses a large number of genes that are expressed in differentiating cells including a group of differentiation promoting TFs involved in leaf development. We show that WUS directly binds to the regulatory regions of... (More)
In animal systems, master regulatory transcription factors (TFs) mediate stem cell maintenance through a direct transcriptional repression of differentiation promoting TFs. Whether similar mechanisms operate in plants is not known. In plants, shoot apical meristems serve as reservoirs of stem cells that provide cells for all above ground organs. WUSCHEL, a homeodomain TF produced in cells of the niche, migrates into adjacent cells where it specifies stem cells. Through high-resolution genomic analysis, we show that WUSCHEL represses a large number of genes that are expressed in differentiating cells including a group of differentiation promoting TFs involved in leaf development. We show that WUS directly binds to the regulatory regions of differentiation promoting TFs; KANADI1, KANADI2, ASYMMETRICLEAVES2 and YABBY3 to repress their expression. Predictions from a computational model, supported by live imaging, reveal that WUS-mediated repression prevents premature differentiation of stem cell progenitors, being part of a minimal regulatory network for meristem maintenance. Our work shows that direct transcriptional repression of differentiation promoting TFs is an evolutionarily conserved logic for stem cell regulation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
central zone, CLAVATA3, shoot apical meristem, stem cell niche, WUSCHEL
in
Molecular Systems Biology
volume
9
pages
654 - 654
publisher
Nature Publishing Group
external identifiers
  • wos:000318693000012
  • scopus:84876520570
ISSN
1744-4292
DOI
10.1038/msb.2013.8
language
English
LU publication?
yes
id
42e396d8-e933-42b1-a28a-07ce586ab6f1 (old id 3815521)
date added to LUP
2013-06-25 15:53:54
date last changed
2019-09-17 02:38:11
@article{42e396d8-e933-42b1-a28a-07ce586ab6f1,
  abstract     = {In animal systems, master regulatory transcription factors (TFs) mediate stem cell maintenance through a direct transcriptional repression of differentiation promoting TFs. Whether similar mechanisms operate in plants is not known. In plants, shoot apical meristems serve as reservoirs of stem cells that provide cells for all above ground organs. WUSCHEL, a homeodomain TF produced in cells of the niche, migrates into adjacent cells where it specifies stem cells. Through high-resolution genomic analysis, we show that WUSCHEL represses a large number of genes that are expressed in differentiating cells including a group of differentiation promoting TFs involved in leaf development. We show that WUS directly binds to the regulatory regions of differentiation promoting TFs; KANADI1, KANADI2, ASYMMETRICLEAVES2 and YABBY3 to repress their expression. Predictions from a computational model, supported by live imaging, reveal that WUS-mediated repression prevents premature differentiation of stem cell progenitors, being part of a minimal regulatory network for meristem maintenance. Our work shows that direct transcriptional repression of differentiation promoting TFs is an evolutionarily conserved logic for stem cell regulation.},
  author       = {Yadav, Ram Kishor and Perales, Mariano and Gruel, Jeremy and Ohno, Carolyn and Heisler, Marcus and Girke, Thomas and Jönsson, Henrik and Reddy, G. Venugopala},
  issn         = {1744-4292},
  keyword      = {central zone,CLAVATA3,shoot apical meristem,stem cell niche,WUSCHEL},
  language     = {eng},
  pages        = {654--654},
  publisher    = {Nature Publishing Group},
  series       = {Molecular Systems Biology},
  title        = {Plant stem cell maintenance involves direct transcriptional repression of differentiation program},
  url          = {http://dx.doi.org/10.1038/msb.2013.8},
  volume       = {9},
  year         = {2013},
}