Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules
(2014) In Biosensors & Bioelectronics 53. p.82-89- Abstract
- The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. (C) 2013 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4319391
- author
- Hazarika, Pompi ; Behrendt, Jonathan M. ; Petersson, Linn LU ; Wingren, Christer LU and Turner, Michael L.
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Self-assembled monolayer (SAM), Silicon, Photo-pattern, Proteins, Biomarker detection
- in
- Biosensors & Bioelectronics
- volume
- 53
- pages
- 82 - 89
- publisher
- Elsevier
- external identifiers
-
- wos:000329881100014
- scopus:84885437336
- pmid:24121227
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2013.09.001
- language
- English
- LU publication?
- yes
- id
- b91c23c0-98af-4ae7-88b0-ab00e25a259f (old id 4319391)
- date added to LUP
- 2016-04-01 14:24:41
- date last changed
- 2022-01-28 00:29:45
@article{b91c23c0-98af-4ae7-88b0-ab00e25a259f, abstract = {{The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. (C) 2013 Elsevier B.V. All rights reserved.}}, author = {{Hazarika, Pompi and Behrendt, Jonathan M. and Petersson, Linn and Wingren, Christer and Turner, Michael L.}}, issn = {{1873-4235}}, keywords = {{Self-assembled monolayer (SAM); Silicon; Photo-pattern; Proteins; Biomarker detection}}, language = {{eng}}, pages = {{82--89}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules}}, url = {{http://dx.doi.org/10.1016/j.bios.2013.09.001}}, doi = {{10.1016/j.bios.2013.09.001}}, volume = {{53}}, year = {{2014}}, }