Advanced

Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules

Hazarika, Pompi; Behrendt, Jonathan M.; Petersson, Linn LU ; Wingren, Christer LU and Turner, Michael L. (2014) In Biosensors & Bioelectronics 53. p.82-89
Abstract
The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. (C) 2013 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Self-assembled monolayer (SAM), Silicon, Photo-pattern, Proteins, Biomarker detection
in
Biosensors & Bioelectronics
volume
53
pages
82 - 89
publisher
Elsevier
external identifiers
  • wos:000329881100014
  • scopus:84885437336
ISSN
1873-4235
DOI
10.1016/j.bios.2013.09.001
language
English
LU publication?
yes
id
b91c23c0-98af-4ae7-88b0-ab00e25a259f (old id 4319391)
date added to LUP
2014-02-26 12:18:17
date last changed
2017-01-01 06:13:55
@article{b91c23c0-98af-4ae7-88b0-ab00e25a259f,
  abstract     = {The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. (C) 2013 Elsevier B.V. All rights reserved.},
  author       = {Hazarika, Pompi and Behrendt, Jonathan M. and Petersson, Linn and Wingren, Christer and Turner, Michael L.},
  issn         = {1873-4235},
  keyword      = {Self-assembled monolayer (SAM),Silicon,Photo-pattern,Proteins,Biomarker detection},
  language     = {eng},
  pages        = {82--89},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules},
  url          = {http://dx.doi.org/10.1016/j.bios.2013.09.001},
  volume       = {53},
  year         = {2014},
}