High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy
(2012) In Environmental Science & Technology 46. p.3950-3956- Abstract
- Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled... (More)
- Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4332237
- author
- Vestergren, Johan ; Vincent, Andrea G. ; Jansson, Mats ; Persson, Per LU ; Istedt, Ulrik ; Groebner, Gerhard ; Giesler, Reiner and Schleucher, Juergen
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Environmental Science & Technology
- volume
- 46
- pages
- 3950 - 3956
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:84859298288
- pmid:22394413
- ISSN
- 1520-5851
- DOI
- 10.1021/es204016h
- language
- English
- LU publication?
- no
- additional info
- 7
- id
- e8286a97-bc95-4d73-b344-4c7680efad12 (old id 4332237)
- date added to LUP
- 2016-04-01 14:26:54
- date last changed
- 2022-04-22 03:18:55
@article{e8286a97-bc95-4d73-b344-4c7680efad12,
abstract = {{Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.}},
author = {{Vestergren, Johan and Vincent, Andrea G. and Jansson, Mats and Persson, Per and Istedt, Ulrik and Groebner, Gerhard and Giesler, Reiner and Schleucher, Juergen}},
issn = {{1520-5851}},
language = {{eng}},
pages = {{3950--3956}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Environmental Science & Technology}},
title = {{High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy}},
url = {{http://dx.doi.org/10.1021/es204016h}},
doi = {{10.1021/es204016h}},
volume = {{46}},
year = {{2012}},
}