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Cell-specific chemotyping and multivariate imaging by combined FT-IR microspectroscopy and orthogonal projections to latent structures (OPLS) analysis reveals the chemical landscape of secondary xylem

Gorzsas, Andras; Stenlund, Hans; Persson, Per LU ; Trygg, Johan and Sundberg, Bjorn (2011) In Plant Journal 66. p.903-914
Abstract
P>Fourier-transform infrared (FT-IR) spectroscopy combined with microscopy enables chemical information to be acquired from native plant cell walls with high spatial resolution. Combined with a 64 x 64 focal plane array (FPA) detector, 4096 spectra can be simultaneously obtained from a 0.3 x 0.3 mm image; each spectrum represents a compositional and structural 'fingerprint' of all cell wall components. For optimal use and analysis of such a large amount of information, multivariate approaches are preferred. Here, FT-IR microspectroscopy with FPA detection is combined with orthogonal projections to latent structures discriminant analysis (OPLS-DA). This allows for: (i) the extraction of spectra from single cell types, (ii) identification... (More)
P>Fourier-transform infrared (FT-IR) spectroscopy combined with microscopy enables chemical information to be acquired from native plant cell walls with high spatial resolution. Combined with a 64 x 64 focal plane array (FPA) detector, 4096 spectra can be simultaneously obtained from a 0.3 x 0.3 mm image; each spectrum represents a compositional and structural 'fingerprint' of all cell wall components. For optimal use and analysis of such a large amount of information, multivariate approaches are preferred. Here, FT-IR microspectroscopy with FPA detection is combined with orthogonal projections to latent structures discriminant analysis (OPLS-DA). This allows for: (i) the extraction of spectra from single cell types, (ii) identification and characterization of different chemotypes using the full spectral information, and (iii) further visualization of the pattern of identified chemotypes by multivariate imaging. As proof of concept, the chemotypes of Populus tremula xylem cell types are described. The approach revealed unknown features about chemical plasticity and patterns of lignin composition in wood fibers that would have remained hidden in the dataset with traditional data analysis. The applicability of the method to Arabidopsis xylem and its usefulness in mutant chemotyping is also demonstrated. The methodological approach is not limited to xylem tissues but can be applied to any plant organ/tissue also using other techniques such as Raman and UV microspectroscopy. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Journal
volume
66
pages
903 - 914
publisher
Wiley-Blackwell
external identifiers
  • scopus:79957626252
ISSN
1365-313X
DOI
10.1111/j.1365-313X.2011.04542.x
language
English
LU publication?
no
id
37d6b68e-c0cc-4b88-b982-2c1e46634572 (old id 4332307)
date added to LUP
2014-03-04 09:46:22
date last changed
2017-10-29 03:55:41
@article{37d6b68e-c0cc-4b88-b982-2c1e46634572,
  abstract     = {P>Fourier-transform infrared (FT-IR) spectroscopy combined with microscopy enables chemical information to be acquired from native plant cell walls with high spatial resolution. Combined with a 64 x 64 focal plane array (FPA) detector, 4096 spectra can be simultaneously obtained from a 0.3 x 0.3 mm image; each spectrum represents a compositional and structural 'fingerprint' of all cell wall components. For optimal use and analysis of such a large amount of information, multivariate approaches are preferred. Here, FT-IR microspectroscopy with FPA detection is combined with orthogonal projections to latent structures discriminant analysis (OPLS-DA). This allows for: (i) the extraction of spectra from single cell types, (ii) identification and characterization of different chemotypes using the full spectral information, and (iii) further visualization of the pattern of identified chemotypes by multivariate imaging. As proof of concept, the chemotypes of Populus tremula xylem cell types are described. The approach revealed unknown features about chemical plasticity and patterns of lignin composition in wood fibers that would have remained hidden in the dataset with traditional data analysis. The applicability of the method to Arabidopsis xylem and its usefulness in mutant chemotyping is also demonstrated. The methodological approach is not limited to xylem tissues but can be applied to any plant organ/tissue also using other techniques such as Raman and UV microspectroscopy.},
  author       = {Gorzsas, Andras and Stenlund, Hans and Persson, Per and Trygg, Johan and Sundberg, Bjorn},
  issn         = {1365-313X},
  language     = {eng},
  pages        = {903--914},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {Cell-specific chemotyping and multivariate imaging by combined FT-IR microspectroscopy and orthogonal projections to latent structures (OPLS) analysis reveals the chemical landscape of secondary xylem},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2011.04542.x},
  volume       = {66},
  year         = {2011},
}