Advanced

Identification of an estrogen receptor gene in the natural freshwater snail Bithynia tentaculata

Hultin, Cecilia LU ; Hallgren, Per LU ; Persson, Anders LU and Hansson, Maria LU (2014) In Gene 540(1). p.26-31
Abstract
Mollusks have received increasing interest in ecotoxicological studies but so far the available scientific analyses of how their genes are affected by anthropogenic pollutants are scarce. The focus of this study is to identify an estrogen receptor (er) gene in the common prosobranch snail Bithynia tentaculata and to test a hypothesis that 17α-Ethinylestradiol (EE2) will modulate er gene expression after short-term exposure. We set up exposure experiments with a total of 144 snails, which were collected from a natural population in southern Sweden. Snails were exposed to either 10 ng/L or 100 ng/L EE2 during 24 h and/or 72 h. From the isolated B. tentaculata RNA we successfully identified and characterized a novel er gene and phylogenetic... (More)
Mollusks have received increasing interest in ecotoxicological studies but so far the available scientific analyses of how their genes are affected by anthropogenic pollutants are scarce. The focus of this study is to identify an estrogen receptor (er) gene in the common prosobranch snail Bithynia tentaculata and to test a hypothesis that 17α-Ethinylestradiol (EE2) will modulate er gene expression after short-term exposure. We set up exposure experiments with a total of 144 snails, which were collected from a natural population in southern Sweden. Snails were exposed to either 10 ng/L or 100 ng/L EE2 during 24 h and/or 72 h. From the isolated B. tentaculata RNA we successfully identified and characterized a novel er gene and phylogenetic analyses strongly indicate that the Bithynia er gene is an ortholog to the human ERα (ESR1, NR3A1). We found a significant interaction between EE2-dose and exposure duration on the er's gene expression (Two-way ANOVA; p = 0.04). We also found a significant difference in the gene expression of the er when comparing the control and 100 ng/L treatment groups after 72 h in female snails (One-way ANOVA; p = 0.047). The results from this study should be useful for future field-related studies of estrogen receptors in natural populations of mollusks. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Estrogen receptor 17α-Ethinylestradiol Mollusk Bithynia
in
Gene
volume
540
issue
1
pages
26 - 31
publisher
Elsevier
external identifiers
  • pmid:24583164
  • wos:000334141000005
  • scopus:84895871872
ISSN
1879-0038
DOI
10.1016/j.gene.2014.02.039
language
English
LU publication?
yes
id
91cbd101-5b9d-47ca-9628-b186bcbb953b (old id 4333442)
date added to LUP
2014-05-05 15:28:05
date last changed
2017-06-25 03:07:24
@article{91cbd101-5b9d-47ca-9628-b186bcbb953b,
  abstract     = {Mollusks have received increasing interest in ecotoxicological studies but so far the available scientific analyses of how their genes are affected by anthropogenic pollutants are scarce. The focus of this study is to identify an estrogen receptor (er) gene in the common prosobranch snail Bithynia tentaculata and to test a hypothesis that 17α-Ethinylestradiol (EE2) will modulate er gene expression after short-term exposure. We set up exposure experiments with a total of 144 snails, which were collected from a natural population in southern Sweden. Snails were exposed to either 10 ng/L or 100 ng/L EE2 during 24 h and/or 72 h. From the isolated B. tentaculata RNA we successfully identified and characterized a novel er gene and phylogenetic analyses strongly indicate that the Bithynia er gene is an ortholog to the human ERα (ESR1, NR3A1). We found a significant interaction between EE2-dose and exposure duration on the er's gene expression (Two-way ANOVA; p = 0.04). We also found a significant difference in the gene expression of the er when comparing the control and 100 ng/L treatment groups after 72 h in female snails (One-way ANOVA; p = 0.047). The results from this study should be useful for future field-related studies of estrogen receptors in natural populations of mollusks.},
  author       = {Hultin, Cecilia and Hallgren, Per and Persson, Anders and Hansson, Maria},
  issn         = {1879-0038},
  keyword      = {Estrogen receptor 17α-Ethinylestradiol Mollusk Bithynia},
  language     = {eng},
  number       = {1},
  pages        = {26--31},
  publisher    = {Elsevier},
  series       = {Gene},
  title        = {Identification of an estrogen receptor gene in the natural freshwater snail Bithynia tentaculata},
  url          = {http://dx.doi.org/10.1016/j.gene.2014.02.039},
  volume       = {540},
  year         = {2014},
}