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High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in Automatic Gas Potential Test System.

Moshi, Anselm LU ; Crespo, Carla LU ; Badshah, Malik LU ; Hosea, Kenneth M M; Mshandete, Anthony Manoni and Mattiasson, Bo LU (2014) In Bioresource Technology 156. p.348-356
Abstract
A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava Manihot glaziovii. FB-SSF allowed fermentation of up to 390g/L of starch-derived glucose achieving high bioethanol concentration of up to 190g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava M. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl® and AMG®). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived... (More)
A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava Manihot glaziovii. FB-SSF allowed fermentation of up to 390g/L of starch-derived glucose achieving high bioethanol concentration of up to 190g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava M. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl® and AMG®). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived stoichiometrically from the CO2 registered in the AMPTS software correlated positively with samples analysed by HPLC (R(2)=0.99). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioresource Technology
volume
156
pages
348 - 356
publisher
Elsevier
external identifiers
  • pmid:24534761
  • wos:000334828500048
  • scopus:84894062261
ISSN
1873-2976
DOI
10.1016/j.biortech.2013.12.082
language
English
LU publication?
yes
id
36def7d2-995f-4124-87aa-3f2e0018eace (old id 4334522)
date added to LUP
2014-03-06 12:38:13
date last changed
2017-10-08 03:18:56
@article{36def7d2-995f-4124-87aa-3f2e0018eace,
  abstract     = {A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava Manihot glaziovii. FB-SSF allowed fermentation of up to 390g/L of starch-derived glucose achieving high bioethanol concentration of up to 190g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava M. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl® and AMG®). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived stoichiometrically from the CO2 registered in the AMPTS software correlated positively with samples analysed by HPLC (R(2)=0.99).},
  author       = {Moshi, Anselm and Crespo, Carla and Badshah, Malik and Hosea, Kenneth M M and Mshandete, Anthony Manoni and Mattiasson, Bo},
  issn         = {1873-2976},
  language     = {eng},
  pages        = {348--356},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in Automatic Gas Potential Test System.},
  url          = {http://dx.doi.org/10.1016/j.biortech.2013.12.082},
  volume       = {156},
  year         = {2014},
}