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Influence of systemic bisphosphonate treatment on mechanical properties of BMP-induced calluses in a rat fracture model: Comparison of three-point bending and twisting test.

Bosemark, Per LU ; Isaksson, Hanna and Tägil, Magnus LU (2014) In Journal of Orthopaedic Research 32(5). p.721-726
Abstract
The combination of autograft, BMP and bisphosphonate has been shown to produce strong calluses. In this study, without autograft we investigate the effect of bisphosphonate treatment on BMP-induced calluses, both in bending and in rotation. Sprague-Dawley rats (n = 42) underwent femoral osteotomy and BMP-7 treatment. At 2 weeks an injection of saline or bisphosphonate was administered. The animals were sacrificed after 6 weeks. Both femurs were tested in either three-point bending or twisting. All femurs healed. BMP + bisphosphonate-treatment led to larger calluses (p < 0.05) and in three-point bending, higher ultimate force (p < 0.01) and greater stiffness (p < 0.05) than BMP alone. The BMP + bisphosphonate group was nearly 60%... (More)
The combination of autograft, BMP and bisphosphonate has been shown to produce strong calluses. In this study, without autograft we investigate the effect of bisphosphonate treatment on BMP-induced calluses, both in bending and in rotation. Sprague-Dawley rats (n = 42) underwent femoral osteotomy and BMP-7 treatment. At 2 weeks an injection of saline or bisphosphonate was administered. The animals were sacrificed after 6 weeks. Both femurs were tested in either three-point bending or twisting. All femurs healed. BMP + bisphosphonate-treatment led to larger calluses (p < 0.05) and in three-point bending, higher ultimate force (p < 0.01) and greater stiffness (p < 0.05) than BMP alone. The BMP + bisphosphonate group was nearly 60% stronger than controls, while the BMP group did not reach the strength (p < 0.05) nor stiffness (p < 0.01) of the controls. In the twisting test, similar trends were found but less pronounced. Three-point bending produced transverse callus associated fractures, whereas the twisting test produced spiral fractures, located in the structurally weaker distal femur. BMP + bisphosphonate-treatment produces calluses that are mechanically superior to calluses induced by BMP alone, when tested both in three-point bending and in twisting. For the mechanical evaluation of pharmacologically enhanced calluses with breaking strengths exceeding the native bone, the bending test is recommended. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Orthopaedic Research
volume
32
issue
5
pages
721 - 726
publisher
John Wiley & Sons
external identifiers
  • pmid:24522981
  • wos:000332842300014
  • scopus:84899438157
ISSN
1554-527X
DOI
10.1002/jor.22599
language
English
LU publication?
yes
id
411f2fbf-fa98-46de-ae5a-4ada7dd0da28 (old id 4334761)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24522981?dopt=Abstract
date added to LUP
2014-03-06 01:40:17
date last changed
2017-04-09 03:15:34
@article{411f2fbf-fa98-46de-ae5a-4ada7dd0da28,
  abstract     = {The combination of autograft, BMP and bisphosphonate has been shown to produce strong calluses. In this study, without autograft we investigate the effect of bisphosphonate treatment on BMP-induced calluses, both in bending and in rotation. Sprague-Dawley rats (n = 42) underwent femoral osteotomy and BMP-7 treatment. At 2 weeks an injection of saline or bisphosphonate was administered. The animals were sacrificed after 6 weeks. Both femurs were tested in either three-point bending or twisting. All femurs healed. BMP + bisphosphonate-treatment led to larger calluses (p &lt; 0.05) and in three-point bending, higher ultimate force (p &lt; 0.01) and greater stiffness (p &lt; 0.05) than BMP alone. The BMP + bisphosphonate group was nearly 60% stronger than controls, while the BMP group did not reach the strength (p &lt; 0.05) nor stiffness (p &lt; 0.01) of the controls. In the twisting test, similar trends were found but less pronounced. Three-point bending produced transverse callus associated fractures, whereas the twisting test produced spiral fractures, located in the structurally weaker distal femur. BMP + bisphosphonate-treatment produces calluses that are mechanically superior to calluses induced by BMP alone, when tested both in three-point bending and in twisting. For the mechanical evaluation of pharmacologically enhanced calluses with breaking strengths exceeding the native bone, the bending test is recommended. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.},
  author       = {Bosemark, Per and Isaksson, Hanna and Tägil, Magnus},
  issn         = {1554-527X},
  language     = {eng},
  number       = {5},
  pages        = {721--726},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Orthopaedic Research},
  title        = {Influence of systemic bisphosphonate treatment on mechanical properties of BMP-induced calluses in a rat fracture model: Comparison of three-point bending and twisting test.},
  url          = {http://dx.doi.org/10.1002/jor.22599},
  volume       = {32},
  year         = {2014},
}