Influence of systemic bisphosphonate treatment on mechanical properties of BMP-induced calluses in a rat fracture model: Comparison of three-point bending and twisting test.
(2014) In Journal of Orthopaedic Research 32(5). p.721-726- Abstract
- The combination of autograft, BMP and bisphosphonate has been shown to produce strong calluses. In this study, without autograft we investigate the effect of bisphosphonate treatment on BMP-induced calluses, both in bending and in rotation. Sprague-Dawley rats (n = 42) underwent femoral osteotomy and BMP-7 treatment. At 2 weeks an injection of saline or bisphosphonate was administered. The animals were sacrificed after 6 weeks. Both femurs were tested in either three-point bending or twisting. All femurs healed. BMP + bisphosphonate-treatment led to larger calluses (p < 0.05) and in three-point bending, higher ultimate force (p < 0.01) and greater stiffness (p < 0.05) than BMP alone. The BMP + bisphosphonate group was nearly 60%... (More)
- The combination of autograft, BMP and bisphosphonate has been shown to produce strong calluses. In this study, without autograft we investigate the effect of bisphosphonate treatment on BMP-induced calluses, both in bending and in rotation. Sprague-Dawley rats (n = 42) underwent femoral osteotomy and BMP-7 treatment. At 2 weeks an injection of saline or bisphosphonate was administered. The animals were sacrificed after 6 weeks. Both femurs were tested in either three-point bending or twisting. All femurs healed. BMP + bisphosphonate-treatment led to larger calluses (p < 0.05) and in three-point bending, higher ultimate force (p < 0.01) and greater stiffness (p < 0.05) than BMP alone. The BMP + bisphosphonate group was nearly 60% stronger than controls, while the BMP group did not reach the strength (p < 0.05) nor stiffness (p < 0.01) of the controls. In the twisting test, similar trends were found but less pronounced. Three-point bending produced transverse callus associated fractures, whereas the twisting test produced spiral fractures, located in the structurally weaker distal femur. BMP + bisphosphonate-treatment produces calluses that are mechanically superior to calluses induced by BMP alone, when tested both in three-point bending and in twisting. For the mechanical evaluation of pharmacologically enhanced calluses with breaking strengths exceeding the native bone, the bending test is recommended. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4334761
- author
- Bosemark, Per LU ; Isaksson, Hanna and Tägil, Magnus LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Orthopaedic Research
- volume
- 32
- issue
- 5
- pages
- 721 - 726
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:24522981
- wos:000332842300014
- scopus:84899438157
- pmid:24522981
- ISSN
- 1554-527X
- DOI
- 10.1002/jor.22599
- language
- English
- LU publication?
- yes
- id
- 411f2fbf-fa98-46de-ae5a-4ada7dd0da28 (old id 4334761)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24522981?dopt=Abstract
- date added to LUP
- 2016-04-01 10:37:34
- date last changed
- 2022-02-17 19:48:14
@article{411f2fbf-fa98-46de-ae5a-4ada7dd0da28,
abstract = {{The combination of autograft, BMP and bisphosphonate has been shown to produce strong calluses. In this study, without autograft we investigate the effect of bisphosphonate treatment on BMP-induced calluses, both in bending and in rotation. Sprague-Dawley rats (n = 42) underwent femoral osteotomy and BMP-7 treatment. At 2 weeks an injection of saline or bisphosphonate was administered. The animals were sacrificed after 6 weeks. Both femurs were tested in either three-point bending or twisting. All femurs healed. BMP + bisphosphonate-treatment led to larger calluses (p < 0.05) and in three-point bending, higher ultimate force (p < 0.01) and greater stiffness (p < 0.05) than BMP alone. The BMP + bisphosphonate group was nearly 60% stronger than controls, while the BMP group did not reach the strength (p < 0.05) nor stiffness (p < 0.01) of the controls. In the twisting test, similar trends were found but less pronounced. Three-point bending produced transverse callus associated fractures, whereas the twisting test produced spiral fractures, located in the structurally weaker distal femur. BMP + bisphosphonate-treatment produces calluses that are mechanically superior to calluses induced by BMP alone, when tested both in three-point bending and in twisting. For the mechanical evaluation of pharmacologically enhanced calluses with breaking strengths exceeding the native bone, the bending test is recommended. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.}},
author = {{Bosemark, Per and Isaksson, Hanna and Tägil, Magnus}},
issn = {{1554-527X}},
language = {{eng}},
number = {{5}},
pages = {{721--726}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Orthopaedic Research}},
title = {{Influence of systemic bisphosphonate treatment on mechanical properties of BMP-induced calluses in a rat fracture model: Comparison of three-point bending and twisting test.}},
url = {{http://dx.doi.org/10.1002/jor.22599}},
doi = {{10.1002/jor.22599}},
volume = {{32}},
year = {{2014}},
}