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Evaluation of composition and mineral structure of callus tissue in rat femoral fracture.

Turunen, Mikael J; Lages, Sebastian; Labrador, Ana; Olsson, Ulf; Tägil, Magnus LU ; Jurvelin, Jukka S and Isaksson, Hanna (2014) In Journal of Biomedical Optics 19(2).
Abstract
ABSTRACT. Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses from Fourier transform infrared (FTIR) microspectroscopy. Femurs of 12 male Sprague-Dawley rats at 9 weeks of age were fractured and fixed with an intramedullary 1.1 mm K-wire. Fractures were treated with the combinations of bone morphogenetic protein-7 and/or zoledronate. Rats were sacrificed after 6 weeks and both femurs were prepared... (More)
ABSTRACT. Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses from Fourier transform infrared (FTIR) microspectroscopy. Femurs of 12 male Sprague-Dawley rats at 9 weeks of age were fractured and fixed with an intramedullary 1.1 mm K-wire. Fractures were treated with the combinations of bone morphogenetic protein-7 and/or zoledronate. Rats were sacrificed after 6 weeks and both femurs were prepared for FTIR and SAXS analysis. Significant differences were found in the molecular composition and mineral structure between the fracture callus, fracture cortex, and control cortex. The degree of mineralization, collagen maturity, and degree of orientation of the mineral plates were lower in the callus tissue than in the cortices. The results indicate the feasibility of SAXS in the investigation of mineral structure of bone fracture callus and provide complementary information with the composition analyzed with FTIR. Moreover, this study contributes to the limited FTIR and SAXS data in the field. (Less)
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organization
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Contribution to journal
publication status
published
subject
in
Journal of Biomedical Optics
volume
19
issue
2
publisher
Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
external identifiers
  • pmid:24522802
  • wos:000332830900016
  • scopus:84897755947
ISSN
1083-3668
DOI
10.1117/1.JBO.19.2.025003
language
English
LU publication?
yes
id
1c594d14-54d3-4c6f-bc91-1ba1e49bf259 (old id 4334767)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24522802?dopt=Abstract
date added to LUP
2014-03-06 10:04:13
date last changed
2017-10-22 03:32:31
@article{1c594d14-54d3-4c6f-bc91-1ba1e49bf259,
  abstract     = {ABSTRACT. Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses from Fourier transform infrared (FTIR) microspectroscopy. Femurs of 12 male Sprague-Dawley rats at 9 weeks of age were fractured and fixed with an intramedullary 1.1 mm K-wire. Fractures were treated with the combinations of bone morphogenetic protein-7 and/or zoledronate. Rats were sacrificed after 6 weeks and both femurs were prepared for FTIR and SAXS analysis. Significant differences were found in the molecular composition and mineral structure between the fracture callus, fracture cortex, and control cortex. The degree of mineralization, collagen maturity, and degree of orientation of the mineral plates were lower in the callus tissue than in the cortices. The results indicate the feasibility of SAXS in the investigation of mineral structure of bone fracture callus and provide complementary information with the composition analyzed with FTIR. Moreover, this study contributes to the limited FTIR and SAXS data in the field.},
  articleno    = {025003},
  author       = {Turunen, Mikael J and Lages, Sebastian and Labrador, Ana and Olsson, Ulf and Tägil, Magnus and Jurvelin, Jukka S and Isaksson, Hanna},
  issn         = {1083-3668},
  language     = {eng},
  number       = {2},
  publisher    = {Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society},
  series       = {Journal of Biomedical Optics},
  title        = {Evaluation of composition and mineral structure of callus tissue in rat femoral fracture.},
  url          = {http://dx.doi.org/10.1117/1.JBO.19.2.025003},
  volume       = {19},
  year         = {2014},
}