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Antibody array generation and use.

Borrebaeck, Carl LU and Wingren, Christer LU (2014) In Methods in Molecular Biology 1131. p.563-571
Abstract
Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease... (More)
Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease diagnostics, prognostics, evidence-based therapy selection, and disease monitoring. Here, we describe the generation and use of planar antibody arrays for serum protein profiling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
1131
pages
563 - 571
publisher
Springer
external identifiers
  • pmid:24515491
  • scopus:84934438179
ISSN
1940-6029
DOI
10.1007/978-1-62703-992-5_36
language
English
LU publication?
yes
id
f411ee2e-abaf-4a16-b8af-d94116a4ebb9 (old id 4334933)
date added to LUP
2014-03-06 14:25:52
date last changed
2017-10-22 03:03:40
@article{f411ee2e-abaf-4a16-b8af-d94116a4ebb9,
  abstract     = {Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease diagnostics, prognostics, evidence-based therapy selection, and disease monitoring. Here, we describe the generation and use of planar antibody arrays for serum protein profiling.},
  author       = {Borrebaeck, Carl and Wingren, Christer},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {563--571},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Antibody array generation and use.},
  url          = {http://dx.doi.org/10.1007/978-1-62703-992-5_36},
  volume       = {1131},
  year         = {2014},
}