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Combined SAXS/UV-vis/Raman as a Diagnostic and Structure Resolving Tool in Materials and Life Sciences Applications.

Haas, Sylvio LU ; Plivelic, Tomás LU and Dicko, Cedric LU (2014) In The Journal of Physical Chemistry Part B 118(8). p.2264-2273
Abstract
In order to diagnose and fully correlate structural, chemical, and functional features of macromolecules and particles in solution, we propose the integration of spectroscopy and scattering on the same measuring volume and at the same time in a dedicated sample environment with multiple probes. Combined SAXS/UV-vis and SAXS/Raman information are employed to study the radiation damage effect in proteins in solution and the scattering from single wall carbon nanotubes (SWNTs) in SDS dispersion, respectively. In the first case, a clear correlation is observed between the time dependence of the radius of gyration (Rg) of the protein determined by SAXS and the turbidity of the protein solution extracted from simultaneous UV-vis measurements. In... (More)
In order to diagnose and fully correlate structural, chemical, and functional features of macromolecules and particles in solution, we propose the integration of spectroscopy and scattering on the same measuring volume and at the same time in a dedicated sample environment with multiple probes. Combined SAXS/UV-vis and SAXS/Raman information are employed to study the radiation damage effect in proteins in solution and the scattering from single wall carbon nanotubes (SWNTs) in SDS dispersion, respectively. In the first case, a clear correlation is observed between the time dependence of the radius of gyration (Rg) of the protein determined by SAXS and the turbidity of the protein solution extracted from simultaneous UV-vis measurements. In the second case, the ratio of bundled/isolated carbon nanotubes is obtained unambiguously through proper modeling of the scattering data and cross-validated with the Raman information. The uses of convex constraint analysis (CCA) and two-dimensional correlation analyses (2DCOS and 2DHCOS) are introduced to fully explore the combination of data sets from different techniques and to extract unique insights from the sample. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
118
issue
8
pages
2264 - 2273
publisher
The American Chemical Society
external identifiers
  • pmid:24484407
  • wos:000332188300032
  • scopus:84896863728
ISSN
1520-5207
DOI
10.1021/jp412229j
language
English
LU publication?
yes
id
debff519-51a6-417c-b040-6e9c99a9eb48 (old id 4336087)
date added to LUP
2014-03-11 16:52:36
date last changed
2017-08-13 03:15:13
@article{debff519-51a6-417c-b040-6e9c99a9eb48,
  abstract     = {In order to diagnose and fully correlate structural, chemical, and functional features of macromolecules and particles in solution, we propose the integration of spectroscopy and scattering on the same measuring volume and at the same time in a dedicated sample environment with multiple probes. Combined SAXS/UV-vis and SAXS/Raman information are employed to study the radiation damage effect in proteins in solution and the scattering from single wall carbon nanotubes (SWNTs) in SDS dispersion, respectively. In the first case, a clear correlation is observed between the time dependence of the radius of gyration (Rg) of the protein determined by SAXS and the turbidity of the protein solution extracted from simultaneous UV-vis measurements. In the second case, the ratio of bundled/isolated carbon nanotubes is obtained unambiguously through proper modeling of the scattering data and cross-validated with the Raman information. The uses of convex constraint analysis (CCA) and two-dimensional correlation analyses (2DCOS and 2DHCOS) are introduced to fully explore the combination of data sets from different techniques and to extract unique insights from the sample.},
  author       = {Haas, Sylvio and Plivelic, Tomás and Dicko, Cedric},
  issn         = {1520-5207},
  language     = {eng},
  number       = {8},
  pages        = {2264--2273},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Combined SAXS/UV-vis/Raman as a Diagnostic and Structure Resolving Tool in Materials and Life Sciences Applications.},
  url          = {http://dx.doi.org/10.1021/jp412229j},
  volume       = {118},
  year         = {2014},
}