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Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex

Lei, Hongxia; Duarte, Joao M N LU ; Mlynarik, Vladimir; Python, Agathe and Gruetter, Rolf (2010) In Journal of Neuroscience Research 88(2). p.9-413
Abstract

Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances in spectra affecting the quantification. As an alternative, thiopental can be administrated without any solubilizing agents. The aim of the study was to investigate the effect of deep thiopental anesthesia on the neurochemical profile consisting of 19 metabolites and on glucose transport kinetics in vivo in rat cortex compared with alpha-chloralose using localized (1)H NMR spectroscopy. Thiopental was devoid of effects on... (More)

Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances in spectra affecting the quantification. As an alternative, thiopental can be administrated without any solubilizing agents. The aim of the study was to investigate the effect of deep thiopental anesthesia on the neurochemical profile consisting of 19 metabolites and on glucose transport kinetics in vivo in rat cortex compared with alpha-chloralose using localized (1)H NMR spectroscopy. Thiopental was devoid of effects on the neurochemical profile, except for the elevated glucose at a given plasma glucose level resulting from thiopental-induced depression of glucose consumption at isoelectrical condition. Over the entire range of plasma glucose levels, steady-state glucose concentrations were increased on average by 48% +/- 8%, implying that an effect of deep thiopental anesthesia on the transport rate relative to cerebral glucose consumption ratio was increased by 47% +/- 8% compared with light alpha-chloralose-anesthetized rats. We conclude that the thiopental-induced isoelectrical condition in rat cortex significantly affected glucose contents by depressing brain metabolism, which remained substantial at isoelectricity.

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publication status
published
keywords
Anesthetics, Intravenous, Animals, Blood Glucose, Cerebral Cortex, Chloralose, Glucose, Homeostasis, Isoelectric Point, Kinetics, Magnetic Resonance Spectroscopy, Male, Protons, Rats, Rats, Sprague-Dawley, Thiopental, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Neuroscience Research
volume
88
issue
2
pages
9 - 413
publisher
John Wiley & Sons
external identifiers
  • scopus:74049124295
ISSN
1097-4547
DOI
10.1002/jnr.22212
language
English
LU publication?
no
id
434600ae-953b-47b6-810f-52f2a80a0027
date added to LUP
2017-10-19 15:19:38
date last changed
2018-05-29 10:28:39
@article{434600ae-953b-47b6-810f-52f2a80a0027,
  abstract     = {<p>Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances in spectra affecting the quantification. As an alternative, thiopental can be administrated without any solubilizing agents. The aim of the study was to investigate the effect of deep thiopental anesthesia on the neurochemical profile consisting of 19 metabolites and on glucose transport kinetics in vivo in rat cortex compared with alpha-chloralose using localized (1)H NMR spectroscopy. Thiopental was devoid of effects on the neurochemical profile, except for the elevated glucose at a given plasma glucose level resulting from thiopental-induced depression of glucose consumption at isoelectrical condition. Over the entire range of plasma glucose levels, steady-state glucose concentrations were increased on average by 48% +/- 8%, implying that an effect of deep thiopental anesthesia on the transport rate relative to cerebral glucose consumption ratio was increased by 47% +/- 8% compared with light alpha-chloralose-anesthetized rats. We conclude that the thiopental-induced isoelectrical condition in rat cortex significantly affected glucose contents by depressing brain metabolism, which remained substantial at isoelectricity.</p>},
  author       = {Lei, Hongxia and Duarte, Joao M N and Mlynarik, Vladimir and Python, Agathe and Gruetter, Rolf},
  issn         = {1097-4547},
  keyword      = {Anesthetics, Intravenous,Animals,Blood Glucose,Cerebral Cortex,Chloralose,Glucose,Homeostasis,Isoelectric Point,Kinetics,Magnetic Resonance Spectroscopy,Male,Protons,Rats,Rats, Sprague-Dawley,Thiopental,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {02},
  number       = {2},
  pages        = {9--413},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Neuroscience Research},
  title        = {Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex},
  url          = {http://dx.doi.org/10.1002/jnr.22212},
  volume       = {88},
  year         = {2010},
}