ATG7 is dispensable for LC3-PE conjugation in thioglycolate-elicited mouse peritoneal macrophages
Vujić, Nemanja ; Bradić, Ivan ; Goeritzer, Madeleine ; Kuentzel, Katharina B LU
; Rainer, Silvia
; Kratky, Dagmar
and Radović, Branislav
(2021)
In Autophagy
17(11).
p.3402-3407
- Abstract
Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient (-/-) and atg7-/-macrophages, we provide evidence that loss of ATG5 but not of ATG7 resulted in LC3-II depletion. Accumulation of LC3-II in elicited atg7-/- macrophages in response to bafilomycin A1 validated these data. Furthermore, complete loss of ATG3 in atg7-/- macrophages demonstrated that ATG7 and ATG3 are dispensable for LC3-PE conjugation. In contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7-/- macrophages exhibited no LC3-II, even under... (More)
Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient (-/-) and atg7-/-macrophages, we provide evidence that loss of ATG5 but not of ATG7 resulted in LC3-II depletion. Accumulation of LC3-II in elicited atg7-/- macrophages in response to bafilomycin A1 validated these data. Furthermore, complete loss of ATG3 in atg7-/- macrophages demonstrated that ATG7 and ATG3 are dispensable for LC3-PE conjugation. In contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7-/- macrophages exhibited no LC3-II, even under inflammatory stimuli in vitro. Hence, the macrophage metabolic status dictates the level of LC3-PE conjugation with a supportive but nonessential role of ATG7, disclosing the eukaryotic exception from the LC3 lipidation model based on yeast data. Abbreviations: ATG: autophagy-related; BM: bone marrow; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; PE: phosphatidylethanolamine.
(Less)
- author
- Vujić, Nemanja
; Bradić, Ivan
; Goeritzer, Madeleine
; Kuentzel, Katharina B
LU
; Rainer, Silvia
; Kratky, Dagmar
and Radović, Branislav
- publishing date
- 2021-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Autophagy/physiology, Autophagy-Related Protein 5/deficiency, Autophagy-Related Protein 7/deficiency, Lipid Metabolism, Macrolides/pharmacology, Macrophages/drug effects, Macrophages, Peritoneal/drug effects, Mice, Mice, Knockout, Microtubule-Associated Proteins/metabolism, Phosphatidylethanolamines/metabolism, Thioglycolates/pharmacology
- in
- Autophagy
- volume
- 17
- issue
- 11
- pages
- 3402 - 3407
- publisher
- Landes Bioscience
- external identifiers
-
- pmid:33459130
- scopus:85100187777
- ISSN
- 1554-8635
- DOI
- 10.1080/15548627.2021.1874132
- language
- English
- LU publication?
- no
- id
- 4375a5e1-686c-4e24-846a-fb5b563b31cc
- date added to LUP
- 2022-10-10 16:26:27
- date last changed
- 2024-12-13 10:37:57
@article{4375a5e1-686c-4e24-846a-fb5b563b31cc,
abstract = {{<p>Thioglycolate-elicited macrophages exhibit abundant conjugation of LC3 with PE (LC3-II). Among other autophagy-related (ATG) proteins, it is proposed that, like in yeast, both ATG5 and ATG7 are essential for LC3 conjugation. Using atg5-deficient (-/-) and atg7-/-macrophages, we provide evidence that loss of ATG5 but not of ATG7 resulted in LC3-II depletion. Accumulation of LC3-II in elicited atg7-/- macrophages in response to bafilomycin A1 validated these data. Furthermore, complete loss of ATG3 in atg7-/- macrophages demonstrated that ATG7 and ATG3 are dispensable for LC3-PE conjugation. In contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7-/- macrophages exhibited no LC3-II, even under inflammatory stimuli in vitro. Hence, the macrophage metabolic status dictates the level of LC3-PE conjugation with a supportive but nonessential role of ATG7, disclosing the eukaryotic exception from the LC3 lipidation model based on yeast data. Abbreviations: ATG: autophagy-related; BM: bone marrow; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; PE: phosphatidylethanolamine.</p>}},
author = {{Vujić, Nemanja and Bradić, Ivan and Goeritzer, Madeleine and Kuentzel, Katharina B and Rainer, Silvia and Kratky, Dagmar and Radović, Branislav}},
issn = {{1554-8635}},
keywords = {{Animals; Autophagy/physiology; Autophagy-Related Protein 5/deficiency; Autophagy-Related Protein 7/deficiency; Lipid Metabolism; Macrolides/pharmacology; Macrophages/drug effects; Macrophages, Peritoneal/drug effects; Mice; Mice, Knockout; Microtubule-Associated Proteins/metabolism; Phosphatidylethanolamines/metabolism; Thioglycolates/pharmacology}},
language = {{eng}},
number = {{11}},
pages = {{3402--3407}},
publisher = {{Landes Bioscience}},
series = {{Autophagy}},
title = {{ATG7 is dispensable for LC3-PE conjugation in thioglycolate-elicited mouse peritoneal macrophages}},
url = {{http://dx.doi.org/10.1080/15548627.2021.1874132}},
doi = {{10.1080/15548627.2021.1874132}},
volume = {{17}},
year = {{2021}},
}