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Molecular Motor Propelled Filaments Reveal Light-Guiding in Nanowire Arrays for Enhanced Biosensing

ten Siethoff, Lasse; Lard, Mercy LU ; Generosi, Johanna LU ; Andersson, Hakan S.; Linke, Heiner LU and Mansson, Alf (2014) In Nano Letters 14(2). p.737-742
Abstract
Semiconductor nanowire arrays offer significant potential for biosensing applications with optical read-out due to their high surface area and due to the unique optical properties of one-dimensional materials. A challenge for optical read-out of analyte-binding to the nanowires is the need to efficiently collect and detect light from a three-dimensional volume. Here we show that light from fluorophores attached along Several mu m long vertical Al2O3 coated gallium phosphide nanowires couples into the wires, is guided along them and emitted at the tip. This enables effective collection of light emitted by fluorescent analytes located at different focal planes along the nanowire. We unequivocally demonstrate the light-guiding effect using a... (More)
Semiconductor nanowire arrays offer significant potential for biosensing applications with optical read-out due to their high surface area and due to the unique optical properties of one-dimensional materials. A challenge for optical read-out of analyte-binding to the nanowires is the need to efficiently collect and detect light from a three-dimensional volume. Here we show that light from fluorophores attached along Several mu m long vertical Al2O3 coated gallium phosphide nanowires couples into the wires, is guided along them and emitted at the tip. This enables effective collection of light emitted by fluorescent analytes located at different focal planes along the nanowire. We unequivocally demonstrate the light-guiding effect using a novel method whereby the changes in emitted fluorescence intensity are observed when fluorescent cytoskeletal filaments are propelled by molecular motors along the wires. The findings are discussed in relation to nanobiosensor developments, other nanotechnological applications, and fundamental studies of motor function. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nanobiotechnology, in vitro motility assay, photonics, actin, myosin, actomyosin
in
Nano Letters
volume
14
issue
2
pages
737 - 742
publisher
The American Chemical Society
external identifiers
  • wos:000331343900052
  • scopus:84894188778
ISSN
1530-6992
DOI
10.1021/nl404032k
language
English
LU publication?
yes
id
ba3215a1-abf2-4f75-9d54-0aaeee27f376 (old id 4376433)
date added to LUP
2014-04-23 11:33:21
date last changed
2017-01-01 06:35:54
@article{ba3215a1-abf2-4f75-9d54-0aaeee27f376,
  abstract     = {Semiconductor nanowire arrays offer significant potential for biosensing applications with optical read-out due to their high surface area and due to the unique optical properties of one-dimensional materials. A challenge for optical read-out of analyte-binding to the nanowires is the need to efficiently collect and detect light from a three-dimensional volume. Here we show that light from fluorophores attached along Several mu m long vertical Al2O3 coated gallium phosphide nanowires couples into the wires, is guided along them and emitted at the tip. This enables effective collection of light emitted by fluorescent analytes located at different focal planes along the nanowire. We unequivocally demonstrate the light-guiding effect using a novel method whereby the changes in emitted fluorescence intensity are observed when fluorescent cytoskeletal filaments are propelled by molecular motors along the wires. The findings are discussed in relation to nanobiosensor developments, other nanotechnological applications, and fundamental studies of motor function.},
  author       = {ten Siethoff, Lasse and Lard, Mercy and Generosi, Johanna and Andersson, Hakan S. and Linke, Heiner and Mansson, Alf},
  issn         = {1530-6992},
  keyword      = {Nanobiotechnology,in vitro motility assay,photonics,actin,myosin,actomyosin},
  language     = {eng},
  number       = {2},
  pages        = {737--742},
  publisher    = {The American Chemical Society},
  series       = {Nano Letters},
  title        = {Molecular Motor Propelled Filaments Reveal Light-Guiding in Nanowire Arrays for Enhanced Biosensing},
  url          = {http://dx.doi.org/10.1021/nl404032k},
  volume       = {14},
  year         = {2014},
}