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Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model

Sigar, Ira M.; Schripsema, Justin H.; Wang, Yibing; Clarke, Ian N.; Cutcliffe, Lesley T.; Seth-Smith, Helena M. B.; Thomson, Nicholas R.; Bjartling, Carina LU ; Unemo, Magnus and Persson, Kenneth LU , et al. (2014) In Pathogens and Disease 70(1). p.61-69
Abstract
We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as... (More)
We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo. (Less)
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Contribution to journal
publication status
published
subject
keywords
Chlamydia, plasmid, mouse, virulence, infection, genome
in
Pathogens and Disease
volume
70
issue
1
pages
61 - 69
publisher
Wiley-Blackwell
external identifiers
  • wos:000331213000007
  • scopus:84901995326
ISSN
2049-632X
DOI
10.1111/2049-632X.12086
language
English
LU publication?
yes
id
c79558a4-fa12-46b9-839a-cdfc497de05e (old id 4376455)
date added to LUP
2014-04-07 09:21:00
date last changed
2017-07-02 03:02:17
@article{c79558a4-fa12-46b9-839a-cdfc497de05e,
  abstract     = {We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.},
  author       = {Sigar, Ira M. and Schripsema, Justin H. and Wang, Yibing and Clarke, Ian N. and Cutcliffe, Lesley T. and Seth-Smith, Helena M. B. and Thomson, Nicholas R. and Bjartling, Carina and Unemo, Magnus and Persson, Kenneth and Ramsey, Kyle H.},
  issn         = {2049-632X},
  keyword      = {Chlamydia,plasmid,mouse,virulence,infection,genome},
  language     = {eng},
  number       = {1},
  pages        = {61--69},
  publisher    = {Wiley-Blackwell},
  series       = {Pathogens and Disease},
  title        = {Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model},
  url          = {http://dx.doi.org/10.1111/2049-632X.12086},
  volume       = {70},
  year         = {2014},
}