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Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production

Aragao, Rosa LU ; Zaushitsyna, Oksana LU ; Berillo, Dmitry LU ; Scaccia, Nazareno LU ; Mattiasson, Bo LU and Kirsebom, Harald LU (2014) In Process Biochemistry 49(1). p.10-18
Abstract
In this study, a new cell immobilization technique is presented. Cells of Clostridium acetobutylicum DSM 792 form a macroporous aggregate through cryogelation with concomitant crosslinking together with activated polyethyleneimine (PEI) and poly(vinyl alcohol) (PVA). The cell based cryogel presents a highly porous, elastic structure with walls consisting of densely packed crosslinked cells. The immobilization process maintained the viability of cells as new bacterial cells were observed when gel-plugs were incubated in liquid medium, glucose was consumed and solvent production was observed. Solvent production was improved 2.7-fold in immobilized cells in comparison to free cells. It was possible to reuse the gel-plugs 3-5 times in partial... (More)
In this study, a new cell immobilization technique is presented. Cells of Clostridium acetobutylicum DSM 792 form a macroporous aggregate through cryogelation with concomitant crosslinking together with activated polyethyleneimine (PEI) and poly(vinyl alcohol) (PVA). The cell based cryogel presents a highly porous, elastic structure with walls consisting of densely packed crosslinked cells. The immobilization process maintained the viability of cells as new bacterial cells were observed when gel-plugs were incubated in liquid medium, glucose was consumed and solvent production was observed. Solvent production was improved 2.7-fold in immobilized cells in comparison to free cells. It was possible to reuse the gel-plugs 3-5 times in partial or completely fresh medium, reaching a maximum butanol concentration in the broth of 18.2 g/l and yield of 0.41 (g/g) in one of the cycles. The use of cells based cryogels can be a good alternative for improvement of acetone-butanol-ethanol (ABE) process as cells are immobilized in a macroporous structure with low limitations for mass transfer with potential for high yield production. (C) 2013 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell immobilization, Cryogel, Butanol, Clostridium acetobutylicum
in
Process Biochemistry
volume
49
issue
1
pages
10 - 18
publisher
Elsevier
external identifiers
  • wos:000331156100002
  • scopus:84891833306
ISSN
1873-3298
DOI
10.1016/j.procbio.2013.09.027
language
English
LU publication?
yes
id
6fa224fa-11f5-4381-adcd-1d161123b896 (old id 4376574)
date added to LUP
2014-04-14 10:47:48
date last changed
2017-08-13 03:16:18
@article{6fa224fa-11f5-4381-adcd-1d161123b896,
  abstract     = {In this study, a new cell immobilization technique is presented. Cells of Clostridium acetobutylicum DSM 792 form a macroporous aggregate through cryogelation with concomitant crosslinking together with activated polyethyleneimine (PEI) and poly(vinyl alcohol) (PVA). The cell based cryogel presents a highly porous, elastic structure with walls consisting of densely packed crosslinked cells. The immobilization process maintained the viability of cells as new bacterial cells were observed when gel-plugs were incubated in liquid medium, glucose was consumed and solvent production was observed. Solvent production was improved 2.7-fold in immobilized cells in comparison to free cells. It was possible to reuse the gel-plugs 3-5 times in partial or completely fresh medium, reaching a maximum butanol concentration in the broth of 18.2 g/l and yield of 0.41 (g/g) in one of the cycles. The use of cells based cryogels can be a good alternative for improvement of acetone-butanol-ethanol (ABE) process as cells are immobilized in a macroporous structure with low limitations for mass transfer with potential for high yield production. (C) 2013 Elsevier Ltd. All rights reserved.},
  author       = {Aragao, Rosa and Zaushitsyna, Oksana and Berillo, Dmitry and Scaccia, Nazareno and Mattiasson, Bo and Kirsebom, Harald},
  issn         = {1873-3298},
  keyword      = {Cell immobilization,Cryogel,Butanol,Clostridium acetobutylicum},
  language     = {eng},
  number       = {1},
  pages        = {10--18},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production},
  url          = {http://dx.doi.org/10.1016/j.procbio.2013.09.027},
  volume       = {49},
  year         = {2014},
}