Advanced

Enriched housing down-regulates the Toll-like receptor 2 response in the mouse brain after experimental stroke.

Quattromani, Miriana LU ; Cordeau, Pierre; Ruscher, Karsten LU ; Kriz, Jasna and Wieloch, Tadeusz LU (2014) In Neurobiology of Disease 66(Mar 6). p.66-73
Abstract
Post-ischemic inflammation plays an important role in the evolution of brain injury, recovery and repair after stroke. Housing rodents in an enriched environment provides multisensory stimulation to the brain and enhances functional recovery after experimental stroke, also depressing the release of cytokines and chemokines in the peri-infarct. In order to identify targets for late stroke treatment, we studied the dynamics of inflammation and the contribution of resident Toll-like receptor 2 (TLR2) expressing microglia cells. We took advantage of the biophotonic/bioluminescent imaging technique using the reporter mouse-expressing luciferase and GFP reporter genes under transcriptional control of the murine TLR2 promoter (TLR2-luc/GFP mice)... (More)
Post-ischemic inflammation plays an important role in the evolution of brain injury, recovery and repair after stroke. Housing rodents in an enriched environment provides multisensory stimulation to the brain and enhances functional recovery after experimental stroke, also depressing the release of cytokines and chemokines in the peri-infarct. In order to identify targets for late stroke treatment, we studied the dynamics of inflammation and the contribution of resident Toll-like receptor 2 (TLR2) expressing microglia cells. We took advantage of the biophotonic/bioluminescent imaging technique using the reporter mouse-expressing luciferase and GFP reporter genes under transcriptional control of the murine TLR2 promoter (TLR2-luc/GFP mice) for non-invasive in vivo analysis of TLR2 activation/response in photothrombotic stroke after differential housing. Real-time imaging at 1day after stroke, revealed up-regulation of TLR2 in response to photothrombotic stroke that subsequently declined over time of recovery (14days). The inflammatory response was persistently down-regulated within days of enriched housing, enhancing recovery of lost sensori-motor function in TLR2-luc mice without affecting infarct size. The number of YM1-expressing microglia in the peri-infarct and areas remote from the infarct was also markedly attenuated. Using a live imaging approach, we demonstrate that multisensory stimulation rapidly, persistently and generally attenuates brain inflammation after experimental stroke, reducing the TLR2 response and leading to improved neurological outcome. TLR2-expressing microglia cells may provide targets for new stroke therapeutics. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Neurobiology of Disease
volume
66
issue
Mar 6
pages
66 - 73
publisher
Elsevier
external identifiers
  • pmid:24613658
  • wos:000335098600006
  • scopus:84897887809
ISSN
0969-9961
DOI
10.1016/j.nbd.2014.02.010
language
English
LU publication?
yes
id
e16a581a-e702-4586-812a-8e352d6bb47e (old id 4383523)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24613658?dopt=Abstract
date added to LUP
2014-04-02 21:41:57
date last changed
2017-07-09 03:16:34
@article{e16a581a-e702-4586-812a-8e352d6bb47e,
  abstract     = {Post-ischemic inflammation plays an important role in the evolution of brain injury, recovery and repair after stroke. Housing rodents in an enriched environment provides multisensory stimulation to the brain and enhances functional recovery after experimental stroke, also depressing the release of cytokines and chemokines in the peri-infarct. In order to identify targets for late stroke treatment, we studied the dynamics of inflammation and the contribution of resident Toll-like receptor 2 (TLR2) expressing microglia cells. We took advantage of the biophotonic/bioluminescent imaging technique using the reporter mouse-expressing luciferase and GFP reporter genes under transcriptional control of the murine TLR2 promoter (TLR2-luc/GFP mice) for non-invasive in vivo analysis of TLR2 activation/response in photothrombotic stroke after differential housing. Real-time imaging at 1day after stroke, revealed up-regulation of TLR2 in response to photothrombotic stroke that subsequently declined over time of recovery (14days). The inflammatory response was persistently down-regulated within days of enriched housing, enhancing recovery of lost sensori-motor function in TLR2-luc mice without affecting infarct size. The number of YM1-expressing microglia in the peri-infarct and areas remote from the infarct was also markedly attenuated. Using a live imaging approach, we demonstrate that multisensory stimulation rapidly, persistently and generally attenuates brain inflammation after experimental stroke, reducing the TLR2 response and leading to improved neurological outcome. TLR2-expressing microglia cells may provide targets for new stroke therapeutics.},
  author       = {Quattromani, Miriana and Cordeau, Pierre and Ruscher, Karsten and Kriz, Jasna and Wieloch, Tadeusz},
  issn         = {0969-9961},
  language     = {eng},
  number       = {Mar 6},
  pages        = {66--73},
  publisher    = {Elsevier},
  series       = {Neurobiology of Disease},
  title        = {Enriched housing down-regulates the Toll-like receptor 2 response in the mouse brain after experimental stroke.},
  url          = {http://dx.doi.org/10.1016/j.nbd.2014.02.010},
  volume       = {66},
  year         = {2014},
}