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Regeneration in vitro of the adult frog sciatic sensory axons

Edström, Anders LU ; Edbladh, Magnus LU ; Ekström, Per LU and Remgård, Pär (1990) In Restorative Neurology and Neuroscience 1(3-4). p.261-266
Abstract

The adult frog sciatic nerve offers several advantages as an in vitro model to study nerve regeneration. The nerve with the attached dorsal root ganglia can easily be isolated and incubated in a culture medium for several days. If the nerve is subjected to a crush immediately after dissection there is a delay of 3.4 days after which the sensory axons start to regenerate into the distal nerve stump at a constant rate of about 1.1 mm · day−1 in serum-containing and 1.0 mm · day−1 in serum-free medium. Serum-free cultures may be used in future studies to examine the effect of various neurotrophic factors. The existence of an accurate method for examining the outgrowth distance, based on axonal transport of labelled... (More)

The adult frog sciatic nerve offers several advantages as an in vitro model to study nerve regeneration. The nerve with the attached dorsal root ganglia can easily be isolated and incubated in a culture medium for several days. If the nerve is subjected to a crush immediately after dissection there is a delay of 3.4 days after which the sensory axons start to regenerate into the distal nerve stump at a constant rate of about 1.1 mm · day−1 in serum-containing and 1.0 mm · day−1 in serum-free medium. Serum-free cultures may be used in future studies to examine the effect of various neurotrophic factors. The existence of an accurate method for examining the outgrowth distance, based on axonal transport of labelled proteins, contributes to the attractiveness of the model. A compartmental culture system permits separate exposure of the ganglia and the nerve to different agents. Taking advantage of this, pharmacological studies suggest that Schwann cells produce signals, dependent on newly transcribed RNA, which transform the preparation into a growth state. The present model system offers favourable conditions to learn more about the early events and also the subsequent steps of the regeneration process. © 1990 IOS Press. All rights reserved.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adult frog, In vitro, Nerve regeneration, Sciatic nerve
in
Restorative Neurology and Neuroscience
volume
1
issue
3-4
pages
6 pages
publisher
IOS Press
external identifiers
  • Scopus:84974754315
ISSN
0922-6028
DOI
10.3233/RNN-1990-13413
language
English
LU publication?
yes
id
4384c457-f4ba-4d55-b462-5a0cd5d6420a
date added to LUP
2016-12-07 14:34:37
date last changed
2017-01-27 15:23:59
@article{4384c457-f4ba-4d55-b462-5a0cd5d6420a,
  abstract     = {<p>The adult frog sciatic nerve offers several advantages as an in vitro model to study nerve regeneration. The nerve with the attached dorsal root ganglia can easily be isolated and incubated in a culture medium for several days. If the nerve is subjected to a crush immediately after dissection there is a delay of 3.4 days after which the sensory axons start to regenerate into the distal nerve stump at a constant rate of about 1.1 mm · day<sup>−1</sup> in serum-containing and 1.0 mm · day<sup>−1</sup> in serum-free medium. Serum-free cultures may be used in future studies to examine the effect of various neurotrophic factors. The existence of an accurate method for examining the outgrowth distance, based on axonal transport of labelled proteins, contributes to the attractiveness of the model. A compartmental culture system permits separate exposure of the ganglia and the nerve to different agents. Taking advantage of this, pharmacological studies suggest that Schwann cells produce signals, dependent on newly transcribed RNA, which transform the preparation into a growth state. The present model system offers favourable conditions to learn more about the early events and also the subsequent steps of the regeneration process. © 1990 IOS Press. All rights reserved.</p>},
  author       = {Edström, Anders and Edbladh, Magnus and Ekström, Per and Remgård, Pär},
  issn         = {0922-6028},
  keyword      = {Adult frog,In vitro,Nerve regeneration,Sciatic nerve},
  language     = {eng},
  number       = {3-4},
  pages        = {261--266},
  publisher    = {IOS Press},
  series       = {Restorative Neurology and Neuroscience},
  title        = {Regeneration in vitro of the adult frog sciatic sensory axons},
  url          = {http://dx.doi.org/10.3233/RNN-1990-13413},
  volume       = {1},
  year         = {1990},
}