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Decreased activities of apolipoprotein m promoter are associated with the susceptibility to coronary artery diseases.

Zheng, Lu; Luo, Guanghua; Zhang, Jun; Mu, Qinfeng; Shi, Yuanping; Berggren Söderlund, Maria LU ; Nilsson-Ehle, Peter LU ; Zhang, Xiaoying and Xu, Ning LU (2014) In International Journal of Medical Sciences 11(4). p.365-372
Abstract
The present study investigated the correlation among genetic polymorphisms of the proximal promoter region of apolipoprotein M (apoM) gene, the polymorphisms in relation to apoM expressions and the susceptibility to coronary artery diseases (CAD) in a Han Chinese population. Four common polymorphic sites, i.e., T-1628G, C-1065A, T-855C and T-778C, were confirmed, and a new deletion mutation C-724del was found, in 206 CAD patients and 209 non-CAD patients using direct DNA sequencing analyses. Occurrences of alleles T-1628G, T-855C and C-724del were significantly higher in CAD patients compared to non-CAD patients. Moreover we examined all these polymorphisms in relation to apoM expression by applying luciferase reporter assay. It... (More)
The present study investigated the correlation among genetic polymorphisms of the proximal promoter region of apolipoprotein M (apoM) gene, the polymorphisms in relation to apoM expressions and the susceptibility to coronary artery diseases (CAD) in a Han Chinese population. Four common polymorphic sites, i.e., T-1628G, C-1065A, T-855C and T-778C, were confirmed, and a new deletion mutation C-724del was found, in 206 CAD patients and 209 non-CAD patients using direct DNA sequencing analyses. Occurrences of alleles T-1628G, T-855C and C-724del were significantly higher in CAD patients compared to non-CAD patients. Moreover we examined all these polymorphisms in relation to apoM expression by applying luciferase reporter assay. It demonstrated that constructs -855C and 724del showed obvious decreased luciferase activities, i.e., (0.93±0.15 vs. 2.11±0.15; P=0.012) and (1.13±0.25 vs. 2.11±0.15; P=0.009) respectively, which indicates these two polymorphisms could confer decreased apoM expressions. Meanwhile the occurrences of these two SNP were also significantly higher in the CAD patients than in non-CAD patients. It is therefore reasonable to speculate that down-regulated apoM expressions in relation to these polymorphisms may affect HDL and cholesterol metabolism in vivo and further influence the susceptibility to CAD, although the underlying mechanisms need further investigation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Medical Sciences
volume
11
issue
4
pages
365 - 372
publisher
Ivyspring International Publisher
external identifiers
  • pmid:24578614
  • wos:000334609100008
  • scopus:84896697731
ISSN
1449-1907
DOI
10.7150/ijms.7696
language
English
LU publication?
yes
id
0f661b9f-b343-4aa2-8e57-844e5698bfb0 (old id 4387365)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24578614?dopt=Abstract
date added to LUP
2014-04-01 13:55:31
date last changed
2017-04-23 03:58:54
@article{0f661b9f-b343-4aa2-8e57-844e5698bfb0,
  abstract     = {The present study investigated the correlation among genetic polymorphisms of the proximal promoter region of apolipoprotein M (apoM) gene, the polymorphisms in relation to apoM expressions and the susceptibility to coronary artery diseases (CAD) in a Han Chinese population. Four common polymorphic sites, i.e., T-1628G, C-1065A, T-855C and T-778C, were confirmed, and a new deletion mutation C-724del was found, in 206 CAD patients and 209 non-CAD patients using direct DNA sequencing analyses. Occurrences of alleles T-1628G, T-855C and C-724del were significantly higher in CAD patients compared to non-CAD patients. Moreover we examined all these polymorphisms in relation to apoM expression by applying luciferase reporter assay. It demonstrated that constructs -855C and 724del showed obvious decreased luciferase activities, i.e., (0.93±0.15 vs. 2.11±0.15; P=0.012) and (1.13±0.25 vs. 2.11±0.15; P=0.009) respectively, which indicates these two polymorphisms could confer decreased apoM expressions. Meanwhile the occurrences of these two SNP were also significantly higher in the CAD patients than in non-CAD patients. It is therefore reasonable to speculate that down-regulated apoM expressions in relation to these polymorphisms may affect HDL and cholesterol metabolism in vivo and further influence the susceptibility to CAD, although the underlying mechanisms need further investigation.},
  author       = {Zheng, Lu and Luo, Guanghua and Zhang, Jun and Mu, Qinfeng and Shi, Yuanping and Berggren Söderlund, Maria and Nilsson-Ehle, Peter and Zhang, Xiaoying and Xu, Ning},
  issn         = {1449-1907},
  language     = {eng},
  number       = {4},
  pages        = {365--372},
  publisher    = {Ivyspring International Publisher},
  series       = {International Journal of Medical Sciences},
  title        = {Decreased activities of apolipoprotein m promoter are associated with the susceptibility to coronary artery diseases.},
  url          = {http://dx.doi.org/10.7150/ijms.7696},
  volume       = {11},
  year         = {2014},
}