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Identification of Missing Proteins – Towards the Completion of Human Proteome

Végvári, Ákos LU (2014) p.7-18
Abstract
As the Human Proteome Project has outlined currently many research groups worldwide dedicated resources to focus on the completion of the human proteome catalogue. According to the project directives, not only all products of ca. 20,300 protein coding genes should be identified but also at least one splicing variant, one single nucleotide polymorphism product and some major post-translational modifications of all human proteins. Today, about 22% of the human proteins have never been observed at expression level but most cases their coding genes were found active and transcript level of identification was achieved.

This chapter presents the recent state of the Human Proteome Project and the challenges that the completion of the... (More)
As the Human Proteome Project has outlined currently many research groups worldwide dedicated resources to focus on the completion of the human proteome catalogue. According to the project directives, not only all products of ca. 20,300 protein coding genes should be identified but also at least one splicing variant, one single nucleotide polymorphism product and some major post-translational modifications of all human proteins. Today, about 22% of the human proteins have never been observed at expression level but most cases their coding genes were found active and transcript level of identification was achieved.

This chapter presents the recent state of the Human Proteome Project and the challenges that the completion of the human protein catalogue represents. Furthermore, discuss the most useful techniques, such as mass spectrometric technologies employed together with bioinformatic tools for identification of novel proteoforms. Databases and their functions are reviewed as well as a successful detection of mutant proteins is exemplified. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Human Proteome Project, mass spectrometry, selected reaction monitoring, prostate specific antigen
categories
Popular Science
host publication
Genomics and Proteomics for Clinical Discovery and Development
editor
Marko-Varga, György
pages
7 - 18
publisher
Springer
ISBN
978-94-017-9201-1
DOI
10.1007/978-94-017-9202-8_2
language
English
LU publication?
yes
id
7118f173-9118-4fff-a654-f1a93d0fbb12 (old id 4389964)
date added to LUP
2016-04-04 10:01:10
date last changed
2018-11-21 20:56:15
@inbook{7118f173-9118-4fff-a654-f1a93d0fbb12,
  abstract     = {{As the Human Proteome Project has outlined currently many research groups worldwide dedicated resources to focus on the completion of the human proteome catalogue. According to the project directives, not only all products of ca. 20,300 protein coding genes should be identified but also at least one splicing variant, one single nucleotide polymorphism product and some major post-translational modifications of all human proteins. Today, about 22% of the human proteins have never been observed at expression level but most cases their coding genes were found active and transcript level of identification was achieved.<br/><br>
This chapter presents the recent state of the Human Proteome Project and the challenges that the completion of the human protein catalogue represents. Furthermore, discuss the most useful techniques, such as mass spectrometric technologies employed together with bioinformatic tools for identification of novel proteoforms. Databases and their functions are reviewed as well as a successful detection of mutant proteins is exemplified.}},
  author       = {{Végvári, Ákos}},
  booktitle    = {{Genomics and Proteomics for Clinical Discovery and Development}},
  editor       = {{Marko-Varga, György}},
  isbn         = {{978-94-017-9201-1}},
  keywords     = {{Human Proteome Project; mass spectrometry; selected reaction monitoring; prostate specific antigen}},
  language     = {{eng}},
  pages        = {{7--18}},
  publisher    = {{Springer}},
  title        = {{Identification of Missing Proteins – Towards the Completion of Human Proteome}},
  url          = {{http://dx.doi.org/10.1007/978-94-017-9202-8_2}},
  doi          = {{10.1007/978-94-017-9202-8_2}},
  year         = {{2014}},
}