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Current status and future opportunities for serial crystallography at MAX IV Laboratory

Shilova, Anastasiia LU ; Lebrette, Hugo ; Aurelius, Oskar LU ; Nan, Jie LU ; Welin, Martin LU ; Kovacic, Rebeka ; Ghosh, Swagatha ; Safari, Cecilia ; Friel, Ross J. LU and Milas, Mirko LU , et al. (2020) In Journal of Synchrotron Radiation 27. p.1095-1102
Abstract

Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to... (More)

Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
high-viscosity injectors, macromolecular crystallography, sample delivery, serial crystallography
in
Journal of Synchrotron Radiation
volume
27
pages
8 pages
publisher
International Union of Crystallography
external identifiers
  • scopus:85090295142
  • pmid:32876583
ISSN
0909-0495
DOI
10.1107/S1600577520008735
language
English
LU publication?
yes
id
43911754-906c-41a1-bc0c-c91d5ee83600
date added to LUP
2021-01-12 11:39:15
date last changed
2024-05-02 00:43:59
@article{43911754-906c-41a1-bc0c-c91d5ee83600,
  abstract     = {{<p>Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.</p>}},
  author       = {{Shilova, Anastasiia and Lebrette, Hugo and Aurelius, Oskar and Nan, Jie and Welin, Martin and Kovacic, Rebeka and Ghosh, Swagatha and Safari, Cecilia and Friel, Ross J. and Milas, Mirko and Matej, Zdenek and Hogbom, Martin and Branden, Gisela and Kloos, Marco and Shoeman, Robert L. and Doak, Bruce and Ursby, Thomas and Hakansson, Maria and Logan, Derek T. and Müller, Uwe}},
  issn         = {{0909-0495}},
  keywords     = {{high-viscosity injectors; macromolecular crystallography; sample delivery; serial crystallography}},
  language     = {{eng}},
  month        = {{09}},
  pages        = {{1095--1102}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Synchrotron Radiation}},
  title        = {{Current status and future opportunities for serial crystallography at MAX IV Laboratory}},
  url          = {{http://dx.doi.org/10.1107/S1600577520008735}},
  doi          = {{10.1107/S1600577520008735}},
  volume       = {{27}},
  year         = {{2020}},
}