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A new osmium-polymer modified screen-printed graphene electrode for fructose detection

Antiochia, Riccarda and Gorton, Lo LU (2014) In Sensors and Actuators B: Chemical 195. p.287-293
Abstract
This paper describes the development and performance of the first fructose biosensor based on a commercial screen-printed graphene electrode (SPGE). The electrode was modified with an osmium-polymer, which allowed the efficient wiring of the enzyme fructose dehydrogenase (FDH). The immobilization of both osmium-polymer and FDH was realized in an easy way. Aliquots of 10 mu L, Os-polymer and 10 mu L, FDH were thoroughly mixed with poly(ethylene glycol) (400) diglycidyl ether (PEDGE) and deposited on the electrode surface and left there to dry overnight. The biosensor exhibits a detection limit of 0.8 mu M, a linear range between 0.1 and 8 mM, high sensitivity to fructose (2.15 mu A cm(-2)/mM), good reproducibility (RSD = 1.9%), fast... (More)
This paper describes the development and performance of the first fructose biosensor based on a commercial screen-printed graphene electrode (SPGE). The electrode was modified with an osmium-polymer, which allowed the efficient wiring of the enzyme fructose dehydrogenase (FDH). The immobilization of both osmium-polymer and FDH was realized in an easy way. Aliquots of 10 mu L, Os-polymer and 10 mu L, FDH were thoroughly mixed with poly(ethylene glycol) (400) diglycidyl ether (PEDGE) and deposited on the electrode surface and left there to dry overnight. The biosensor exhibits a detection limit of 0.8 mu M, a linear range between 0.1 and 8 mM, high sensitivity to fructose (2.15 mu A cm(-2)/mM), good reproducibility (RSD = 1.9%), fast response time (3 s) and a stability of 2 months when stored in the freezer. The proposed fructose biosensor was tested in real food samples and validated with a commercial spectrophotometric enzymatic kit. No significant interference was observed with the proposed biosensor. Published by Elsevier B.V. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Osmium redox polymer, dehydrogenase, Screen-printed electrode, Graphene, Fructose, Biosensor
in
Sensors and Actuators B: Chemical
volume
195
pages
287 - 293
publisher
Elsevier
external identifiers
  • wos:000332417600040
  • scopus:84893720555
ISSN
0925-4005
DOI
10.1016/j.snb.2014.01.050
language
English
LU publication?
yes
id
074d5e2e-f509-4273-90c0-cd2519d89127 (old id 4414228)
date added to LUP
2014-04-29 15:42:13
date last changed
2017-09-10 04:10:18
@article{074d5e2e-f509-4273-90c0-cd2519d89127,
  abstract     = {This paper describes the development and performance of the first fructose biosensor based on a commercial screen-printed graphene electrode (SPGE). The electrode was modified with an osmium-polymer, which allowed the efficient wiring of the enzyme fructose dehydrogenase (FDH). The immobilization of both osmium-polymer and FDH was realized in an easy way. Aliquots of 10 mu L, Os-polymer and 10 mu L, FDH were thoroughly mixed with poly(ethylene glycol) (400) diglycidyl ether (PEDGE) and deposited on the electrode surface and left there to dry overnight. The biosensor exhibits a detection limit of 0.8 mu M, a linear range between 0.1 and 8 mM, high sensitivity to fructose (2.15 mu A cm(-2)/mM), good reproducibility (RSD = 1.9%), fast response time (3 s) and a stability of 2 months when stored in the freezer. The proposed fructose biosensor was tested in real food samples and validated with a commercial spectrophotometric enzymatic kit. No significant interference was observed with the proposed biosensor. Published by Elsevier B.V.},
  author       = {Antiochia, Riccarda and Gorton, Lo},
  issn         = {0925-4005},
  keyword      = {Osmium redox polymer,dehydrogenase,Screen-printed electrode,Graphene,Fructose,Biosensor},
  language     = {eng},
  pages        = {287--293},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {A new osmium-polymer modified screen-printed graphene electrode for fructose detection},
  url          = {http://dx.doi.org/10.1016/j.snb.2014.01.050},
  volume       = {195},
  year         = {2014},
}