Determination of blood cell subtype concentrations from frozen whole blood samples using TruCount beads
Langenskiöld, Cecilia ; Mellgren, Karin ; Abrahamsson, Jonas and Bemark, Mats LU
(2018)
In Cytometry Part B - Clinical Cytometry
94(4).
p.660-666
- Abstract
Background: In many studies it would be advantageous if blood samples could be collected and analyzed using flow cytometry at a later stage. Ideally, sample collection should involve little hands-on time, allow for long-term storage, and minimally influence the samples. Methods: Here we establish a flow cytometry antibody panel that can be used to determine granulocytes, monocytes, and lymphocyte subset concentrations in fresh and frozen whole blood using TruCount technology. Results: The panel can be used on fresh whole-blood samples as well as whole-blood samples that have been frozen after mixing with 10% DMSO. Concentrations in frozen and fresh sample is highly correlated both when frozen within 4 h and the day after collection (r ≥... (More)
Background: In many studies it would be advantageous if blood samples could be collected and analyzed using flow cytometry at a later stage. Ideally, sample collection should involve little hands-on time, allow for long-term storage, and minimally influence the samples. Methods: Here we establish a flow cytometry antibody panel that can be used to determine granulocytes, monocytes, and lymphocyte subset concentrations in fresh and frozen whole blood using TruCount technology. Results: The panel can be used on fresh whole-blood samples as well as whole-blood samples that have been frozen after mixing with 10% DMSO. Concentrations in frozen and fresh sample is highly correlated both when frozen within 4 h and the day after collection (r ≥ 0.98), and the estimated concentration in frozen samples was between 91 and 94% of that in fresh samples for all cell types. Conclusion: Using this method whole-blood samples can be frozen using a simple preparation method, and stored long-term before accurate determination of cell concentration. This allows for standardized analysis of the samples at a reference laboratory in multi-center studies.
(Less)
- author
- Langenskiöld, Cecilia
; Mellgren, Karin
; Abrahamsson, Jonas
and Bemark, Mats
LU
- publishing date
- 2018-07
- type
- Contribution to journal
- publication status
- published
- keywords
- absolute cell count, clinical trial, cryopreservation, flow cytometry, immunophenotyping, peripheral blood, TruCount
- in
- Cytometry Part B - Clinical Cytometry
- volume
- 94
- issue
- 4
- pages
- 660 - 666
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:27342079
- scopus:84979784054
- ISSN
- 1552-4949
- DOI
- 10.1002/cyto.b.21390
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2016 International Clinical Cytometry Society
- id
- 44146b79-0977-4973-9441-777ad58e3309
- date added to LUP
- 2023-12-06 16:45:28
- date last changed
- 2024-04-05 07:27:08
@article{44146b79-0977-4973-9441-777ad58e3309,
abstract = {{<p>Background: In many studies it would be advantageous if blood samples could be collected and analyzed using flow cytometry at a later stage. Ideally, sample collection should involve little hands-on time, allow for long-term storage, and minimally influence the samples. Methods: Here we establish a flow cytometry antibody panel that can be used to determine granulocytes, monocytes, and lymphocyte subset concentrations in fresh and frozen whole blood using TruCount technology. Results: The panel can be used on fresh whole-blood samples as well as whole-blood samples that have been frozen after mixing with 10% DMSO. Concentrations in frozen and fresh sample is highly correlated both when frozen within 4 h and the day after collection (r ≥ 0.98), and the estimated concentration in frozen samples was between 91 and 94% of that in fresh samples for all cell types. Conclusion: Using this method whole-blood samples can be frozen using a simple preparation method, and stored long-term before accurate determination of cell concentration. This allows for standardized analysis of the samples at a reference laboratory in multi-center studies.</p>}},
author = {{Langenskiöld, Cecilia and Mellgren, Karin and Abrahamsson, Jonas and Bemark, Mats}},
issn = {{1552-4949}},
keywords = {{absolute cell count; clinical trial; cryopreservation; flow cytometry; immunophenotyping; peripheral blood; TruCount}},
language = {{eng}},
number = {{4}},
pages = {{660--666}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Cytometry Part B - Clinical Cytometry}},
title = {{Determination of blood cell subtype concentrations from frozen whole blood samples using TruCount beads}},
url = {{http://dx.doi.org/10.1002/cyto.b.21390}},
doi = {{10.1002/cyto.b.21390}},
volume = {{94}},
year = {{2018}},
}