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The effects of glucose loading rates on bacterial and fungal growth in soil

Reischke, Stephanie LU ; Rousk, Johannes LU and Bååth, Erland LU (2014) In Soil Biology & Biochemistry 70. p.88-95
Abstract
Microbial activity in soil is usually limited by the availability of carbon (C). Adding an easily available C source, like glucose, has therefore been a common approach to study alleviation of resource limitations. Most such studies have relied on respiration to study microbial dynamics, with few following the explicit growth response. We determined the response in bacterial and fungal growth, as well as respiration, to additions of glucose (0.5-32 mg C g(-1) soil) during up to 6 days, using leucine incorporation for bacterial growth and acetate-in-ergosterol incorporation for fungal growth. A concentration of 2 mg glucose-C g(-1) soil, where the fungal contribution appeared to be small, was also studied with a high time resolution. Adding... (More)
Microbial activity in soil is usually limited by the availability of carbon (C). Adding an easily available C source, like glucose, has therefore been a common approach to study alleviation of resource limitations. Most such studies have relied on respiration to study microbial dynamics, with few following the explicit growth response. We determined the response in bacterial and fungal growth, as well as respiration, to additions of glucose (0.5-32 mg C g(-1) soil) during up to 6 days, using leucine incorporation for bacterial growth and acetate-in-ergosterol incorporation for fungal growth. A concentration of 2 mg glucose-C g(-1) soil, where the fungal contribution appeared to be small, was also studied with a high time resolution. Adding glucose resulted in an initial lag phase of stable respiration and bacterial growth. Bacterial growth was similar to the unamended control, while respiration was 8 fold higher during this period. The 14-h lag phase was followed by an exponential increase for both respiration and bacterial growth, with a similar intrinsic growth rate (mu) of around 0.25 h(-1). After the exponential phase, bacterial growth decreased exponentially. The respiration initially decreased even more rapidly than bacterial growth. At concentrations exceeding 4 mg glucose-C g(-1) the relative stimulation of fungal growth surpassed that of bacteria, with the highest amendment rates, 32 mg C g(-1), resulting in mainly fungal growth. Lower loading rates than 4 mg glucose-C g(-1) appeared to stimulate mainly bacterial growth. (C) 2013 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Substrate loading rates, Glucose, Bacterial growth, Fungal growth, Respiration, H-3-leucine incorporation, C-14-acetate incorporation, Mineralisation, Decomposition
in
Soil Biology & Biochemistry
volume
70
pages
88 - 95
publisher
Elsevier
external identifiers
  • wos:000332439800012
  • scopus:84891895199
ISSN
0038-0717
DOI
10.1016/j.soilbio.2013.12.011
project
Interaction between fungi and bacteria in soil
Effect of environmental factors on fungal and bacterial growth in soil
Microbial carbon-use efficiency
MICCS - Molecular Interactions Controlling soil Carbon Sequestration
language
English
LU publication?
yes
id
cf9221e5-3ee2-4d17-9502-81889d0c9b10 (old id 4417776)
date added to LUP
2014-04-30 12:56:22
date last changed
2017-09-10 04:22:51
@article{cf9221e5-3ee2-4d17-9502-81889d0c9b10,
  abstract     = {Microbial activity in soil is usually limited by the availability of carbon (C). Adding an easily available C source, like glucose, has therefore been a common approach to study alleviation of resource limitations. Most such studies have relied on respiration to study microbial dynamics, with few following the explicit growth response. We determined the response in bacterial and fungal growth, as well as respiration, to additions of glucose (0.5-32 mg C g(-1) soil) during up to 6 days, using leucine incorporation for bacterial growth and acetate-in-ergosterol incorporation for fungal growth. A concentration of 2 mg glucose-C g(-1) soil, where the fungal contribution appeared to be small, was also studied with a high time resolution. Adding glucose resulted in an initial lag phase of stable respiration and bacterial growth. Bacterial growth was similar to the unamended control, while respiration was 8 fold higher during this period. The 14-h lag phase was followed by an exponential increase for both respiration and bacterial growth, with a similar intrinsic growth rate (mu) of around 0.25 h(-1). After the exponential phase, bacterial growth decreased exponentially. The respiration initially decreased even more rapidly than bacterial growth. At concentrations exceeding 4 mg glucose-C g(-1) the relative stimulation of fungal growth surpassed that of bacteria, with the highest amendment rates, 32 mg C g(-1), resulting in mainly fungal growth. Lower loading rates than 4 mg glucose-C g(-1) appeared to stimulate mainly bacterial growth. (C) 2013 Elsevier Ltd. All rights reserved.},
  author       = {Reischke, Stephanie and Rousk, Johannes and Bååth, Erland},
  issn         = {0038-0717},
  keyword      = {Substrate loading rates,Glucose,Bacterial growth,Fungal growth,Respiration,H-3-leucine incorporation,C-14-acetate incorporation,Mineralisation,Decomposition},
  language     = {eng},
  pages        = {88--95},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {The effects of glucose loading rates on bacterial and fungal growth in soil},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2013.12.011},
  volume       = {70},
  year         = {2014},
}