Prediction of IgG1 aggregation in solution.
Ojala, Frida LU ; Degerman, Marcus LU ; Budde Hansen, Thomas ; Broberg Hansen, Ernst and Nilsson, Bernt LU
(2014)
In Biotechnology Journal
9(6).
p.800-804
- Abstract
- Interest in monoclonal antibody aggregation is increasing as aggregates of biopharma-ceuticals can cause an immunogenic response when injected into the body. In this work a stoichiometric reaction model from concentration-time data is developed to predict the dimer ratio in stored antibody solutions over time. IgG1 was incubated at pH from 4.5 to 5.5, salt concentrations from 100 to 600 mmol/kg and protein concentrations of 10.6 to 26.3 g/l; samples were taken at intervals of 20 minutes to five hours over time periods from 4 hours to 7.6 days, and analyzed with size-exclusion chromatography. The experiments showed the formation of dimers from monomers, but no higher order aggregates. Dilution of samples containing dimers led to the... (More)
- Interest in monoclonal antibody aggregation is increasing as aggregates of biopharma-ceuticals can cause an immunogenic response when injected into the body. In this work a stoichiometric reaction model from concentration-time data is developed to predict the dimer ratio in stored antibody solutions over time. IgG1 was incubated at pH from 4.5 to 5.5, salt concentrations from 100 to 600 mmol/kg and protein concentrations of 10.6 to 26.3 g/l; samples were taken at intervals of 20 minutes to five hours over time periods from 4 hours to 7.6 days, and analyzed with size-exclusion chromatography. The experiments showed the formation of dimers from monomers, but no higher order aggregates. Dilution of samples containing dimers led to the reversal of the dimerization reaction. Measurements of the concentrations of each component were made by fitting exponentially modified Gaussian peaks to the chromatograms used to measure the concentrations of the different forms of protein. This stoichiometric reaction model was able to predict the formation of dimers by the antibody studied. The equilibrium constant was found to be dependent on the salt concentration, and the kinetic constant showed a dependence on the pH of the solution. The prediction of the aggregation leads to a possibility of optimizing the conditions in order to prevent the dimer formation and to maximize the monomer concentration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4429533
- author
- Ojala, Frida
LU
; Degerman, Marcus
LU
; Budde Hansen, Thomas
; Broberg Hansen, Ernst
and Nilsson, Bernt
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Journal
- volume
- 9
- issue
- 6
- pages
- 800 - 804
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:24760776
- wos:000337700000010
- scopus:84901691457
- pmid:24760776
- ISSN
- 1860-6768
- DOI
- 10.1002/biot.201400018
- language
- English
- LU publication?
- yes
- id
- 6ce2a22a-9415-4fba-81a4-ebfb4f0b56bd (old id 4429533)
- date added to LUP
- 2016-04-01 10:55:26
- date last changed
- 2024-10-07 16:37:53
@article{6ce2a22a-9415-4fba-81a4-ebfb4f0b56bd,
abstract = {{Interest in monoclonal antibody aggregation is increasing as aggregates of biopharma-ceuticals can cause an immunogenic response when injected into the body. In this work a stoichiometric reaction model from concentration-time data is developed to predict the dimer ratio in stored antibody solutions over time. IgG1 was incubated at pH from 4.5 to 5.5, salt concentrations from 100 to 600 mmol/kg and protein concentrations of 10.6 to 26.3 g/l; samples were taken at intervals of 20 minutes to five hours over time periods from 4 hours to 7.6 days, and analyzed with size-exclusion chromatography. The experiments showed the formation of dimers from monomers, but no higher order aggregates. Dilution of samples containing dimers led to the reversal of the dimerization reaction. Measurements of the concentrations of each component were made by fitting exponentially modified Gaussian peaks to the chromatograms used to measure the concentrations of the different forms of protein. This stoichiometric reaction model was able to predict the formation of dimers by the antibody studied. The equilibrium constant was found to be dependent on the salt concentration, and the kinetic constant showed a dependence on the pH of the solution. The prediction of the aggregation leads to a possibility of optimizing the conditions in order to prevent the dimer formation and to maximize the monomer concentration.}},
author = {{Ojala, Frida and Degerman, Marcus and Budde Hansen, Thomas and Broberg Hansen, Ernst and Nilsson, Bernt}},
issn = {{1860-6768}},
language = {{eng}},
number = {{6}},
pages = {{800--804}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology Journal}},
title = {{Prediction of IgG1 aggregation in solution.}},
url = {{http://dx.doi.org/10.1002/biot.201400018}},
doi = {{10.1002/biot.201400018}},
volume = {{9}},
year = {{2014}},
}