Multi-radionuclide digital autoradiography of the intra-aortic atherosclerotic plaques using a monoclonal antibody targeting oxidized low-density lipoprotein.
(2014) In American journal of nuclear medicine and molecular imaging 4(2). p.172-180- Abstract
- The aim of this study was to use multi-radionuclide autoradiography to compare the different distributions of three radiolabelled tracers in an atherosclerotic mouse model. This method, along with immunohistochemistry, was applied to investigate the intra-aortic distribution of 2-deoxy-2-[(18)F]fluoro-D-glucose ((18)F-FDG), (131)I/(125)I labeled anti-oxidized Low Density Lipoprotein (oxLDL), and non-binding control antibodies. Aortas were isolated from a total of 12 apoB-100/LDL receptor deficient mice 73 h post injection of radioiodine-labeled anti-oxLDL and control antibody and 1 h post injection of (18)F-FDG. A solid-state real-time digital autoradiography system was used to image the slide mounted aortas. Contributions from each... (More)
- The aim of this study was to use multi-radionuclide autoradiography to compare the different distributions of three radiolabelled tracers in an atherosclerotic mouse model. This method, along with immunohistochemistry, was applied to investigate the intra-aortic distribution of 2-deoxy-2-[(18)F]fluoro-D-glucose ((18)F-FDG), (131)I/(125)I labeled anti-oxidized Low Density Lipoprotein (oxLDL), and non-binding control antibodies. Aortas were isolated from a total of 12 apoB-100/LDL receptor deficient mice 73 h post injection of radioiodine-labeled anti-oxLDL and control antibody and 1 h post injection of (18)F-FDG. A solid-state real-time digital autoradiography system was used to image the slide mounted aortas. Contributions from each radionuclide were separated by half-life and emission energy and the aortas were subsequently stained with Oil Red O for plaque to aorta contrast ratios. Immunohistochemical staining was performed to detect anti-oxLDL and control antibody localization. Radiolabeled anti-oxLDL showed increased total activity uptake in the aorta over control antibody and immunohistochemical analysis of plaques indicated increased binding of the specific antibody compared to control. The intra-aortic activity distribution of the anti-oxLDL antibody was however very similar to that of the control antibody although both had higher atherosclerotic plaques to aorta wall ratios than (18)F-FDG. Given the right choice of radionuclides, multi-radionuclide digital autoradiography can be employed to compare several tracers ex vivo in the same animal. The distribution of anti-oxLDL antibodies did not significantly differ from the control antibody but it did appear to have a better plaque to aorta contrast at 73 h post injection than (18)F-FDG at 1 h post injection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4429684
- author
- Örbom, Anders
LU
; Jansson, Bo ; Schiopu, Alexandru LU
; Evans Axelsson, Susan LU
; Nilsson, Jan LU ; Nordin Fredrikson, Gunilla LU and Strand, Sven-Erik LU
- organization
-
- Medical Radiation Physics, Lund
- Cardiovascular Research - Immunity and Atherosclerosis (research group)
- Urological cancer, Malmö (research group)
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- EXODIAB: Excellence of Diabetes Research in Sweden
- EpiHealth: Epidemiology for Health
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American journal of nuclear medicine and molecular imaging
- volume
- 4
- issue
- 2
- pages
- 172 - 180
- publisher
- e-Century Publishing
- external identifiers
-
- pmid:24753983
- ISSN
- 2160-8407
- language
- English
- LU publication?
- yes
- id
- 14defb4b-d210-425d-92cc-4827c10f9a7b (old id 4429684)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24753983?dopt=Abstract
- date added to LUP
- 2016-04-01 13:58:49
- date last changed
- 2020-02-08 02:24:37
@article{14defb4b-d210-425d-92cc-4827c10f9a7b, abstract = {{The aim of this study was to use multi-radionuclide autoradiography to compare the different distributions of three radiolabelled tracers in an atherosclerotic mouse model. This method, along with immunohistochemistry, was applied to investigate the intra-aortic distribution of 2-deoxy-2-[(18)F]fluoro-D-glucose ((18)F-FDG), (131)I/(125)I labeled anti-oxidized Low Density Lipoprotein (oxLDL), and non-binding control antibodies. Aortas were isolated from a total of 12 apoB-100/LDL receptor deficient mice 73 h post injection of radioiodine-labeled anti-oxLDL and control antibody and 1 h post injection of (18)F-FDG. A solid-state real-time digital autoradiography system was used to image the slide mounted aortas. Contributions from each radionuclide were separated by half-life and emission energy and the aortas were subsequently stained with Oil Red O for plaque to aorta contrast ratios. Immunohistochemical staining was performed to detect anti-oxLDL and control antibody localization. Radiolabeled anti-oxLDL showed increased total activity uptake in the aorta over control antibody and immunohistochemical analysis of plaques indicated increased binding of the specific antibody compared to control. The intra-aortic activity distribution of the anti-oxLDL antibody was however very similar to that of the control antibody although both had higher atherosclerotic plaques to aorta wall ratios than (18)F-FDG. Given the right choice of radionuclides, multi-radionuclide digital autoradiography can be employed to compare several tracers ex vivo in the same animal. The distribution of anti-oxLDL antibodies did not significantly differ from the control antibody but it did appear to have a better plaque to aorta contrast at 73 h post injection than (18)F-FDG at 1 h post injection.}}, author = {{Örbom, Anders and Jansson, Bo and Schiopu, Alexandru and Evans Axelsson, Susan and Nilsson, Jan and Nordin Fredrikson, Gunilla and Strand, Sven-Erik}}, issn = {{2160-8407}}, language = {{eng}}, number = {{2}}, pages = {{172--180}}, publisher = {{e-Century Publishing}}, series = {{American journal of nuclear medicine and molecular imaging}}, title = {{Multi-radionuclide digital autoradiography of the intra-aortic atherosclerotic plaques using a monoclonal antibody targeting oxidized low-density lipoprotein.}}, url = {{https://lup.lub.lu.se/search/files/3707326/4731776}}, volume = {{4}}, year = {{2014}}, }