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Circulating CD1c(+) DCs are superior at activating Th2 responses upon Phl p stimulation compared with basophils and pDCs.

Rydnert, Frida; Lundberg, Kristina; Greiff, Lennart LU and Lindstedt, Malin (2014) In Immunology and Cell Biology 92(6). p.557-560
Abstract
The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and basophils in allergen presentation and Th2 activation. By coculturing Phleum pratense (Phl p)-pulsed CD1c(+) mDCs, CD123(+) pDCs, monocyte-derived DCs and basophils with autologous CD4(+) effector memory T cells, we assessed T-cell proliferation as well as the frequency of interleukin-4- and interferon-γ-producing T cells. Interestingly, a Th2-stimulating ability was observed for Phl p-challenged CD1c(+) mDCs and... (More)
The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and basophils in allergen presentation and Th2 activation. By coculturing Phleum pratense (Phl p)-pulsed CD1c(+) mDCs, CD123(+) pDCs, monocyte-derived DCs and basophils with autologous CD4(+) effector memory T cells, we assessed T-cell proliferation as well as the frequency of interleukin-4- and interferon-γ-producing T cells. Interestingly, a Th2-stimulating ability was observed for Phl p-challenged CD1c(+) mDCs and monocyte-derived DCs, while CD123(+) pDCs and basophils did not affect the Th-balance. In addition, both Phl p-pulsed CD1c(+) mDCs and monocyte-derived DCs stimulated increased T-cell proliferation compared to basophils and CD123(+) pDCs. Together, these results point to a prominent role for circulating CD1c(+) mDCs in allergen presentation and augmentation of Th2 responses, making them promising therapeutic targets for Type I hypersensitivity reactions.Immunology and Cell Biology advance online publication, 1 April 2014; doi:10.1038/icb.2014.23. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Immunology and Cell Biology
volume
92
issue
6
pages
557 - 560
publisher
Nature Publishing Group
external identifiers
  • pmid:24687020
  • wos:000338709600013
  • scopus:84904044490
ISSN
1440-1711
DOI
10.1038/icb.2014.23
language
English
LU publication?
yes
id
b3f847e3-77bf-40ad-a67e-4bcab1893775 (old id 4431381)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24687020?dopt=Abstract
date added to LUP
2014-05-07 18:47:39
date last changed
2017-01-01 03:41:44
@article{b3f847e3-77bf-40ad-a67e-4bcab1893775,
  abstract     = {The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and basophils in allergen presentation and Th2 activation. By coculturing Phleum pratense (Phl p)-pulsed CD1c(+) mDCs, CD123(+) pDCs, monocyte-derived DCs and basophils with autologous CD4(+) effector memory T cells, we assessed T-cell proliferation as well as the frequency of interleukin-4- and interferon-γ-producing T cells. Interestingly, a Th2-stimulating ability was observed for Phl p-challenged CD1c(+) mDCs and monocyte-derived DCs, while CD123(+) pDCs and basophils did not affect the Th-balance. In addition, both Phl p-pulsed CD1c(+) mDCs and monocyte-derived DCs stimulated increased T-cell proliferation compared to basophils and CD123(+) pDCs. Together, these results point to a prominent role for circulating CD1c(+) mDCs in allergen presentation and augmentation of Th2 responses, making them promising therapeutic targets for Type I hypersensitivity reactions.Immunology and Cell Biology advance online publication, 1 April 2014; doi:10.1038/icb.2014.23.},
  author       = {Rydnert, Frida and Lundberg, Kristina and Greiff, Lennart and Lindstedt, Malin},
  issn         = {1440-1711},
  language     = {eng},
  number       = {6},
  pages        = {557--560},
  publisher    = {Nature Publishing Group},
  series       = {Immunology and Cell Biology},
  title        = {Circulating CD1c(+) DCs are superior at activating Th2 responses upon Phl p stimulation compared with basophils and pDCs.},
  url          = {http://dx.doi.org/10.1038/icb.2014.23},
  volume       = {92},
  year         = {2014},
}