Circulating CD1c(+) DCs are superior at activating Th2 responses upon Phl p stimulation compared with basophils and pDCs.
(2014) In Immunology and Cell Biology 92(6). p.557-560- Abstract
- The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and basophils in allergen presentation and Th2 activation. By coculturing Phleum pratense (Phl p)-pulsed CD1c(+) mDCs, CD123(+) pDCs, monocyte-derived DCs and basophils with autologous CD4(+) effector memory T cells, we assessed T-cell proliferation as well as the frequency of interleukin-4- and interferon-γ-producing T cells. Interestingly, a Th2-stimulating ability was observed for Phl p-challenged CD1c(+) mDCs and... (More)
- The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and basophils in allergen presentation and Th2 activation. By coculturing Phleum pratense (Phl p)-pulsed CD1c(+) mDCs, CD123(+) pDCs, monocyte-derived DCs and basophils with autologous CD4(+) effector memory T cells, we assessed T-cell proliferation as well as the frequency of interleukin-4- and interferon-γ-producing T cells. Interestingly, a Th2-stimulating ability was observed for Phl p-challenged CD1c(+) mDCs and monocyte-derived DCs, while CD123(+) pDCs and basophils did not affect the Th-balance. In addition, both Phl p-pulsed CD1c(+) mDCs and monocyte-derived DCs stimulated increased T-cell proliferation compared to basophils and CD123(+) pDCs. Together, these results point to a prominent role for circulating CD1c(+) mDCs in allergen presentation and augmentation of Th2 responses, making them promising therapeutic targets for Type I hypersensitivity reactions.Immunology and Cell Biology advance online publication, 1 April 2014; doi:10.1038/icb.2014.23. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4431381
- author
- Rydnert, Frida ; Lundberg, Kristina ; Greiff, Lennart LU and Lindstedt, Malin
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Immunology and Cell Biology
- volume
- 92
- issue
- 6
- pages
- 557 - 560
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:24687020
- wos:000338709600013
- scopus:84904044490
- pmid:24687020
- ISSN
- 1440-1711
- DOI
- 10.1038/icb.2014.23
- language
- English
- LU publication?
- yes
- id
- b3f847e3-77bf-40ad-a67e-4bcab1893775 (old id 4431381)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24687020?dopt=Abstract
- date added to LUP
- 2016-04-01 10:33:10
- date last changed
- 2022-02-17 19:14:32
@article{b3f847e3-77bf-40ad-a67e-4bcab1893775,
abstract = {{The contributing role of circulating human dendritic cell (DC) populations and basophils in the presentation and augmentation of Th2 responses remains to be determined. The present study aimed at elucidating the functional role of CD1c(+) myeloid DCs (mDCs), CD123(+) plasmacytoid DCs (pDCs), monocyte-derived DCs and basophils in allergen presentation and Th2 activation. By coculturing Phleum pratense (Phl p)-pulsed CD1c(+) mDCs, CD123(+) pDCs, monocyte-derived DCs and basophils with autologous CD4(+) effector memory T cells, we assessed T-cell proliferation as well as the frequency of interleukin-4- and interferon-γ-producing T cells. Interestingly, a Th2-stimulating ability was observed for Phl p-challenged CD1c(+) mDCs and monocyte-derived DCs, while CD123(+) pDCs and basophils did not affect the Th-balance. In addition, both Phl p-pulsed CD1c(+) mDCs and monocyte-derived DCs stimulated increased T-cell proliferation compared to basophils and CD123(+) pDCs. Together, these results point to a prominent role for circulating CD1c(+) mDCs in allergen presentation and augmentation of Th2 responses, making them promising therapeutic targets for Type I hypersensitivity reactions.Immunology and Cell Biology advance online publication, 1 April 2014; doi:10.1038/icb.2014.23.}},
author = {{Rydnert, Frida and Lundberg, Kristina and Greiff, Lennart and Lindstedt, Malin}},
issn = {{1440-1711}},
language = {{eng}},
number = {{6}},
pages = {{557--560}},
publisher = {{Nature Publishing Group}},
series = {{Immunology and Cell Biology}},
title = {{Circulating CD1c(+) DCs are superior at activating Th2 responses upon Phl p stimulation compared with basophils and pDCs.}},
url = {{http://dx.doi.org/10.1038/icb.2014.23}},
doi = {{10.1038/icb.2014.23}},
volume = {{92}},
year = {{2014}},
}