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Identification of pili on the surface of Finegoldia magna - A Gram-positive anaerobic cocci.

Murphy, Elizabeth LU ; Janulczyk, Robert LU ; Karlsson, Christofer LU ; Mörgelin, Matthias LU and Frick, Inga-Maria LU (2014) In Anaerobe 27(Mar 29). p.40-49
Abstract
Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint... (More)
Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint infections. In this study, F. magna ALB8 was found to harbour three sortases at the pilus locus, two of which bear high similarity to class C sortases in Streptococcus pneumoniae. Two putative sortase-dependent pili proteins were found in the locus, with one being identified as the major pilus subunit, Fmp1 (F. magna pilus subunit 1), due to its high similarity to other major pilus proteins in prominent Gram-positive pathogens. The presence of sortase-dependent pili was confirmed experimentally through recombinant production of Fmp1 and production of antiserum. The Fmp1 antiserum was used in Western blot to show the presence of a high molecular weight protein ladder, characteristic of the presence of pili, in trypsin released cell wall surface proteins from F. magna. The presence of sortase-dependent pili was visually confirmed by transmission electron microscopy, which showed the binding of gold labelled anti-Fmp1 to individual pilus proteins along the pilus. Furthermore, pili could also be found to bind and interact with keratinocytes in the epidermal layer of human skin, suggesting an adhesive role for pili on F. magna. Our work represents the first description of pilus structures in F. magna. This discovery further elucidates F. magna physiology and allows for additional analysis of host-bacterial interactions in future studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Anaerobe
volume
27
issue
Mar 29
pages
40 - 49
publisher
Elsevier
external identifiers
  • pmid:24685556
  • wos:000337659600009
  • scopus:84898623406
ISSN
1095-8274
DOI
10.1016/j.anaerobe.2014.03.008
language
English
LU publication?
yes
id
070e646c-8072-4721-a079-d1abf1ab9b51 (old id 4431388)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24685556?dopt=Abstract
date added to LUP
2014-05-07 18:49:49
date last changed
2017-11-05 03:13:51
@article{070e646c-8072-4721-a079-d1abf1ab9b51,
  abstract     = {Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint infections. In this study, F. magna ALB8 was found to harbour three sortases at the pilus locus, two of which bear high similarity to class C sortases in Streptococcus pneumoniae. Two putative sortase-dependent pili proteins were found in the locus, with one being identified as the major pilus subunit, Fmp1 (F. magna pilus subunit 1), due to its high similarity to other major pilus proteins in prominent Gram-positive pathogens. The presence of sortase-dependent pili was confirmed experimentally through recombinant production of Fmp1 and production of antiserum. The Fmp1 antiserum was used in Western blot to show the presence of a high molecular weight protein ladder, characteristic of the presence of pili, in trypsin released cell wall surface proteins from F. magna. The presence of sortase-dependent pili was visually confirmed by transmission electron microscopy, which showed the binding of gold labelled anti-Fmp1 to individual pilus proteins along the pilus. Furthermore, pili could also be found to bind and interact with keratinocytes in the epidermal layer of human skin, suggesting an adhesive role for pili on F. magna. Our work represents the first description of pilus structures in F. magna. This discovery further elucidates F. magna physiology and allows for additional analysis of host-bacterial interactions in future studies.},
  author       = {Murphy, Elizabeth and Janulczyk, Robert and Karlsson, Christofer and Mörgelin, Matthias and Frick, Inga-Maria},
  issn         = {1095-8274},
  language     = {eng},
  number       = {Mar 29},
  pages        = {40--49},
  publisher    = {Elsevier},
  series       = {Anaerobe},
  title        = {Identification of pili on the surface of Finegoldia magna - A Gram-positive anaerobic cocci.},
  url          = {http://dx.doi.org/10.1016/j.anaerobe.2014.03.008},
  volume       = {27},
  year         = {2014},
}