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eNOS Activation by HDL Is Impaired in Genetic CETP Deficiency.

Gomaraschi, Monica; Ossoli, Alice; Pozzi, Silvia; Nilsson, Peter LU ; Cefalù, Angelo B; Averna, Maurizio; Kuivenhoven, Jan Albert; Hovingh, G Kees; Veglia, Fabrizio and Franceschini, Guido, et al. (2014) In PLoS ONE 9(5).
Abstract
Mutations in the CETP gene resulting in defective CETP activity have been shown to cause remarkable elevations of plasma HDL-C levels, with the accumulation in plasma of large, buoyant HDL particles enriched in apolipoprotein E. Genetic CETP deficiency thus represents a unique tool to evaluate how structural alterations of HDL impact on HDL atheroprotective functions. Aim of the present study was to assess the ability of HDL obtained from CETP-deficient subjects to protect endothelial cells from the development of endothelial dysfunction. HDL isolated from one homozygous and seven heterozygous carriers of CETP null mutations were evaluated for their ability to down-regulate cytokine-induced cell adhesion molecule expression and to promote... (More)
Mutations in the CETP gene resulting in defective CETP activity have been shown to cause remarkable elevations of plasma HDL-C levels, with the accumulation in plasma of large, buoyant HDL particles enriched in apolipoprotein E. Genetic CETP deficiency thus represents a unique tool to evaluate how structural alterations of HDL impact on HDL atheroprotective functions. Aim of the present study was to assess the ability of HDL obtained from CETP-deficient subjects to protect endothelial cells from the development of endothelial dysfunction. HDL isolated from one homozygous and seven heterozygous carriers of CETP null mutations were evaluated for their ability to down-regulate cytokine-induced cell adhesion molecule expression and to promote NO production in cultured endothelial cells. When compared at the same protein concentration, HDL and HDL3 from carriers proved to be as effective as control HDL and HDL3 in down-regulating cytokine-induced VCAM-1, while carrier HDL2 were more effective than control HDL2 in inhibiting VCAM-1 expression. On the other hand, HDL and HDL fractions from carriers of CETP deficiency were significantly less effective than control HDL and HDL fractions in stimulating NO production, due to a reduced eNOS activating capacity, likely because of a reduced S1P content. In conclusion, the present findings support the notion that genetic CETP deficiency, by affecting HDL particle structure, impacts on HDL vasculoprotective functions. Understanding of these effects might be important for predicting the outcomes of pharmacological CETP inhibition. (Less)
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PLoS ONE
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9
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5
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Public Library of Science
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  • pmid:24830642
  • wos:000336789500010
  • scopus:84901324767
ISSN
1932-6203
DOI
10.1371/journal.pone.0095925
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English
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yes
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d4805ba7-87da-43fc-a911-e2a26ef6648e (old id 4455124)
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http://www.ncbi.nlm.nih.gov/pubmed/24830642?dopt=Abstract
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2014-06-04 20:39:33
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@article{d4805ba7-87da-43fc-a911-e2a26ef6648e,
  abstract     = {Mutations in the CETP gene resulting in defective CETP activity have been shown to cause remarkable elevations of plasma HDL-C levels, with the accumulation in plasma of large, buoyant HDL particles enriched in apolipoprotein E. Genetic CETP deficiency thus represents a unique tool to evaluate how structural alterations of HDL impact on HDL atheroprotective functions. Aim of the present study was to assess the ability of HDL obtained from CETP-deficient subjects to protect endothelial cells from the development of endothelial dysfunction. HDL isolated from one homozygous and seven heterozygous carriers of CETP null mutations were evaluated for their ability to down-regulate cytokine-induced cell adhesion molecule expression and to promote NO production in cultured endothelial cells. When compared at the same protein concentration, HDL and HDL3 from carriers proved to be as effective as control HDL and HDL3 in down-regulating cytokine-induced VCAM-1, while carrier HDL2 were more effective than control HDL2 in inhibiting VCAM-1 expression. On the other hand, HDL and HDL fractions from carriers of CETP deficiency were significantly less effective than control HDL and HDL fractions in stimulating NO production, due to a reduced eNOS activating capacity, likely because of a reduced S1P content. In conclusion, the present findings support the notion that genetic CETP deficiency, by affecting HDL particle structure, impacts on HDL vasculoprotective functions. Understanding of these effects might be important for predicting the outcomes of pharmacological CETP inhibition.},
  articleno    = {e95925},
  author       = {Gomaraschi, Monica and Ossoli, Alice and Pozzi, Silvia and Nilsson, Peter and Cefalù, Angelo B and Averna, Maurizio and Kuivenhoven, Jan Albert and Hovingh, G Kees and Veglia, Fabrizio and Franceschini, Guido and Calabresi, Laura},
  issn         = {1932-6203},
  language     = {eng},
  number       = {5},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {eNOS Activation by HDL Is Impaired in Genetic CETP Deficiency.},
  url          = {http://dx.doi.org/10.1371/journal.pone.0095925},
  volume       = {9},
  year         = {2014},
}