Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies.
(2014) In Analytica Chimica Acta 826. p.69-76- Abstract
- The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (∼80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde... (More)
- The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (∼80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10(-13)M to 1×10(-10)M MC-LR standard with a limit of detection of 2.1×10(-14)M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4455914
- author
- Lebogang, Lesedi LU ; Hedström, Martin LU and Mattiasson, Bo LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytica Chimica Acta
- volume
- 826
- pages
- 69 - 76
- publisher
- Elsevier
- external identifiers
-
- pmid:24793855
- wos:000335452300009
- scopus:84926207040
- pmid:24793855
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2014.03.028
- language
- English
- LU publication?
- yes
- id
- 0dd7832e-9546-4a7d-abbe-62a0c409251d (old id 4455914)
- date added to LUP
- 2016-04-01 10:47:21
- date last changed
- 2022-03-20 00:03:38
@article{0dd7832e-9546-4a7d-abbe-62a0c409251d,
abstract = {{The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (∼80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10(-13)M to 1×10(-10)M MC-LR standard with a limit of detection of 2.1×10(-14)M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle.}},
author = {{Lebogang, Lesedi and Hedström, Martin and Mattiasson, Bo}},
issn = {{1873-4324}},
language = {{eng}},
pages = {{69--76}},
publisher = {{Elsevier}},
series = {{Analytica Chimica Acta}},
title = {{Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies.}},
url = {{http://dx.doi.org/10.1016/j.aca.2014.03.028}},
doi = {{10.1016/j.aca.2014.03.028}},
volume = {{826}},
year = {{2014}},
}