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Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies.

Lebogang, Lesedi LU ; Hedström, Martin LU and Mattiasson, Bo LU (2014) In Analytica Chimica Acta 826. p.69-76
Abstract
The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (∼80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde... (More)
The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (∼80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10(-13)M to 1×10(-10)M MC-LR standard with a limit of detection of 2.1×10(-14)M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
826
pages
69 - 76
publisher
Elsevier
external identifiers
  • pmid:24793855
  • wos:000335452300009
  • scopus:84926207040
ISSN
1873-4324
DOI
10.1016/j.aca.2014.03.028
language
English
LU publication?
yes
id
0dd7832e-9546-4a7d-abbe-62a0c409251d (old id 4455914)
date added to LUP
2014-06-18 14:53:58
date last changed
2017-07-30 03:21:22
@article{0dd7832e-9546-4a7d-abbe-62a0c409251d,
  abstract     = {The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (∼80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10(-13)M to 1×10(-10)M MC-LR standard with a limit of detection of 2.1×10(-14)M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle.},
  author       = {Lebogang, Lesedi and Hedström, Martin and Mattiasson, Bo},
  issn         = {1873-4324},
  language     = {eng},
  pages        = {69--76},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies.},
  url          = {http://dx.doi.org/10.1016/j.aca.2014.03.028},
  volume       = {826},
  year         = {2014},
}