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Sensitivity of HIV-1 primary isolates to human anti-CD40 antibody-mediated suppression is related to co-receptor use

Abayneh, Sisay A ; Ellmark, Peter LU ; Karlsson, Ulf LU ; Andersson, Henrik ; Borrebaeck, Carl LU ; Karlsson, Ingrid LU and Fenyö, Eva Maria LU (2008) In AIDS Research and Human Retroviruses 24(3). p.447-452
Abstract
The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric... (More)
The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric receptors. In comparison, HIV-1 isolates with broader use of CCR5/CXCR4 chimeric receptors or with CXCR4 use were less sensitive to anti-CD40-induced suppression. The results indicate that HIV-1 replication is inhibited by human anti-CD40 monoclonal antibodies through the mechanism of CC-chemokine induction. This effect is thus restricted to HIV-1 isolates sensitive to inhibition by CC-chemokines. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
AIDS Research and Human Retroviruses
volume
24
issue
3
pages
447 - 452
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000254735200014
  • pmid:18373433
  • scopus:41449100085
  • pmid:18373433
ISSN
1931-8405
DOI
10.1089/aid.2007.0216
language
English
LU publication?
yes
id
445aeff0-5d77-4f11-8294-52d320d8b1ed (old id 773857)
date added to LUP
2016-04-01 12:34:39
date last changed
2022-01-27 07:00:42
@article{445aeff0-5d77-4f11-8294-52d320d8b1ed,
  abstract     = {{The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric receptors. In comparison, HIV-1 isolates with broader use of CCR5/CXCR4 chimeric receptors or with CXCR4 use were less sensitive to anti-CD40-induced suppression. The results indicate that HIV-1 replication is inhibited by human anti-CD40 monoclonal antibodies through the mechanism of CC-chemokine induction. This effect is thus restricted to HIV-1 isolates sensitive to inhibition by CC-chemokines.}},
  author       = {{Abayneh, Sisay A and Ellmark, Peter and Karlsson, Ulf and Andersson, Henrik and Borrebaeck, Carl and Karlsson, Ingrid and Fenyö, Eva Maria}},
  issn         = {{1931-8405}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{447--452}},
  publisher    = {{Mary Ann Liebert, Inc.}},
  series       = {{AIDS Research and Human Retroviruses}},
  title        = {{Sensitivity of HIV-1 primary isolates to human anti-CD40 antibody-mediated suppression is related to co-receptor use}},
  url          = {{http://dx.doi.org/10.1089/aid.2007.0216}},
  doi          = {{10.1089/aid.2007.0216}},
  volume       = {{24}},
  year         = {{2008}},
}