Advanced

Young men with low birthweight exhibit decreased plasticity of genome-wide muscle DNA methylation by high-fat overfeeding

Jacobsen, Stine C.; Gillberg, Linn; Bork-Jensen, Jette; Ribel-Madsen, Rasmus; Lara, Ester; Calvanese, Vincenzo; Ling, Charlotte LU ; Fernandez, Agustin F.; Fraga, Mario F. and Poulsen, Pernille, et al. (2014) In Diabetologia 57(6). p.1154-1158
Abstract
Aims/hypothesis The association between low birthweight (LBW) and risk of developing type 2 diabetes may involve epigenetic mechanisms, with skeletal muscle being a prime target tissue. Differential DNA methylation patterns have been observed in single genes in muscle tissue from type 2 diabetic and LBW individuals, and we recently showed multiple DNA methylation changes during short-term high-fat overfeeding in muscle of healthy people. In a randomised crossover study, we analysed genome-wide DNA promoter methylation in skeletal muscle of 17 young LBW men and 23 matched normal birthweight (NBW) men after a control and a 5 day high-fat overfeeding diet. Methods DNA methylation was measured using Illumina's Infinium BeadArray covering... (More)
Aims/hypothesis The association between low birthweight (LBW) and risk of developing type 2 diabetes may involve epigenetic mechanisms, with skeletal muscle being a prime target tissue. Differential DNA methylation patterns have been observed in single genes in muscle tissue from type 2 diabetic and LBW individuals, and we recently showed multiple DNA methylation changes during short-term high-fat overfeeding in muscle of healthy people. In a randomised crossover study, we analysed genome-wide DNA promoter methylation in skeletal muscle of 17 young LBW men and 23 matched normal birthweight (NBW) men after a control and a 5 day high-fat overfeeding diet. Methods DNA methylation was measured using Illumina's Infinium BeadArray covering 27,578 CpG sites representing 14,475 different genes. Results After correction for multiple comparisons, DNA methylation levels were found to be similar in the LBW and NBW groups during the control diet. Whereas widespread DNA methylation changes were observed in the NBW group in response to high-fat overfeeding, only a few methylation changes were seen in the LBW group (chi(2), p < 0.001). Conclusions/interpretation Our results indicate lower DNA methylation plasticity in skeletal muscle from LBW vs NBW men, potentially contributing to understanding the link between LBW and increased risk of type 2 diabetes. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNAmethylation, Epigenetics, High-fat overfeeding, Insulin resistance, Low birthweight, Skeletal muscle, Type 2 diabetes
in
Diabetologia
volume
57
issue
6
pages
1154 - 1158
publisher
Springer Verlag
external identifiers
  • wos:000335922800008
  • scopus:84901188568
ISSN
1432-0428
DOI
10.1007/s00125-014-3198-8
language
English
LU publication?
yes
id
8bcc4ca8-dc4f-48ec-bc42-081275cb69e1 (old id 4469863)
date added to LUP
2014-07-01 07:41:55
date last changed
2017-10-22 03:26:08
@article{8bcc4ca8-dc4f-48ec-bc42-081275cb69e1,
  abstract     = {Aims/hypothesis The association between low birthweight (LBW) and risk of developing type 2 diabetes may involve epigenetic mechanisms, with skeletal muscle being a prime target tissue. Differential DNA methylation patterns have been observed in single genes in muscle tissue from type 2 diabetic and LBW individuals, and we recently showed multiple DNA methylation changes during short-term high-fat overfeeding in muscle of healthy people. In a randomised crossover study, we analysed genome-wide DNA promoter methylation in skeletal muscle of 17 young LBW men and 23 matched normal birthweight (NBW) men after a control and a 5 day high-fat overfeeding diet. Methods DNA methylation was measured using Illumina's Infinium BeadArray covering 27,578 CpG sites representing 14,475 different genes. Results After correction for multiple comparisons, DNA methylation levels were found to be similar in the LBW and NBW groups during the control diet. Whereas widespread DNA methylation changes were observed in the NBW group in response to high-fat overfeeding, only a few methylation changes were seen in the LBW group (chi(2), p &lt; 0.001). Conclusions/interpretation Our results indicate lower DNA methylation plasticity in skeletal muscle from LBW vs NBW men, potentially contributing to understanding the link between LBW and increased risk of type 2 diabetes.},
  author       = {Jacobsen, Stine C. and Gillberg, Linn and Bork-Jensen, Jette and Ribel-Madsen, Rasmus and Lara, Ester and Calvanese, Vincenzo and Ling, Charlotte and Fernandez, Agustin F. and Fraga, Mario F. and Poulsen, Pernille and Brons, Charlotte and Vaag, Allan},
  issn         = {1432-0428},
  keyword      = {DNAmethylation,Epigenetics,High-fat overfeeding,Insulin resistance,Low birthweight,Skeletal muscle,Type 2 diabetes},
  language     = {eng},
  number       = {6},
  pages        = {1154--1158},
  publisher    = {Springer Verlag},
  series       = {Diabetologia},
  title        = {Young men with low birthweight exhibit decreased plasticity of genome-wide muscle DNA methylation by high-fat overfeeding},
  url          = {http://dx.doi.org/10.1007/s00125-014-3198-8},
  volume       = {57},
  year         = {2014},
}