Advanced

Detectability of new psychoactive substances, 'legal highs', in CEDIA, EMIT, and KIMS immunochemical screening assays for drugs of abuse

Beck, Olof; Rausberg, Linnea; Al-Saffar, Yasir; Villen, Tomas; Karlsson, Lennart; Hansson, Therese LU and Helander, Anders (2014) In Drug Testing and Analysis 6(5). p.492-499
Abstract
The increasing number of new psychoactive substances made available for recreational drug use has created a challenge for clinical toxicology and drug testing laboratories. As a consequence, the routine immunoassay drug testing may become less effective due to an increased occurrence of false negative and false positive screening results. This work aimed to extend the knowledge about analytical cross-reactivity of new substances in selected CEDIA, EMIT, and KIMS immunoassays for drugs-of-abuse screening. Urine standards were prepared by spiking blank urine with 45 new substances. Authentic urine samples from intoxication cases identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were also studied. Several new... (More)
The increasing number of new psychoactive substances made available for recreational drug use has created a challenge for clinical toxicology and drug testing laboratories. As a consequence, the routine immunoassay drug testing may become less effective due to an increased occurrence of false negative and false positive screening results. This work aimed to extend the knowledge about analytical cross-reactivity of new substances in selected CEDIA, EMIT, and KIMS immunoassays for drugs-of-abuse screening. Urine standards were prepared by spiking blank urine with 45 new substances. Authentic urine samples from intoxication cases identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were also studied. Several new psychoactive substances were demonstrated to display cross-reactivity in the immunoassays. CEDIA Amphetamine/Ecstasy and EMIT d.a.u. Amphetamine Class tests showed the highest reactivity towards the new drugs, which was expected since many have amphetamine-like structure and activity. In the samples from authentic cases, five new substances displayed 100% detection rate in the CEDIA Amphetamine/Ecstasy test. In conclusion, cross-reactivity data in routine urine drug screening immunoassays for a number of new psychoactive substances not studied before were reported. In both spiked and authentic urine samples, some new substances showed significant cross-reactivity and are thus detectable in the routine screening methods. Copyright (c) 2014 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CEDIA, EMIT, KIMS, immunoassay, urine, drug testing, new psychoactive, substances, drug screening
in
Drug Testing and Analysis
volume
6
issue
5
pages
492 - 499
publisher
Wiley-Blackwell
external identifiers
  • wos:000335491700010
  • scopus:84899977106
ISSN
1942-7611
DOI
10.1002/dta.1641
language
English
LU publication?
yes
id
12bb1ef4-7dec-4020-ad86-b436f3fbd429 (old id 4470583)
date added to LUP
2014-07-01 07:36:52
date last changed
2017-08-13 03:07:18
@article{12bb1ef4-7dec-4020-ad86-b436f3fbd429,
  abstract     = {The increasing number of new psychoactive substances made available for recreational drug use has created a challenge for clinical toxicology and drug testing laboratories. As a consequence, the routine immunoassay drug testing may become less effective due to an increased occurrence of false negative and false positive screening results. This work aimed to extend the knowledge about analytical cross-reactivity of new substances in selected CEDIA, EMIT, and KIMS immunoassays for drugs-of-abuse screening. Urine standards were prepared by spiking blank urine with 45 new substances. Authentic urine samples from intoxication cases identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were also studied. Several new psychoactive substances were demonstrated to display cross-reactivity in the immunoassays. CEDIA Amphetamine/Ecstasy and EMIT d.a.u. Amphetamine Class tests showed the highest reactivity towards the new drugs, which was expected since many have amphetamine-like structure and activity. In the samples from authentic cases, five new substances displayed 100% detection rate in the CEDIA Amphetamine/Ecstasy test. In conclusion, cross-reactivity data in routine urine drug screening immunoassays for a number of new psychoactive substances not studied before were reported. In both spiked and authentic urine samples, some new substances showed significant cross-reactivity and are thus detectable in the routine screening methods. Copyright (c) 2014 John Wiley & Sons, Ltd.},
  author       = {Beck, Olof and Rausberg, Linnea and Al-Saffar, Yasir and Villen, Tomas and Karlsson, Lennart and Hansson, Therese and Helander, Anders},
  issn         = {1942-7611},
  keyword      = {CEDIA,EMIT,KIMS,immunoassay,urine,drug testing,new psychoactive,substances,drug screening},
  language     = {eng},
  number       = {5},
  pages        = {492--499},
  publisher    = {Wiley-Blackwell},
  series       = {Drug Testing and Analysis},
  title        = {Detectability of new psychoactive substances, 'legal highs', in CEDIA, EMIT, and KIMS immunochemical screening assays for drugs of abuse},
  url          = {http://dx.doi.org/10.1002/dta.1641},
  volume       = {6},
  year         = {2014},
}