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Flow-Through-Mediated Surface Immobilization of Sub-Micrometre Particles in Monolithic Cryogels

Hajizadeh, Solmaz LU ; Mattiasson, Bo LU and Kirsebom, Harald LU (2014) In Macromolecular Materials and Engineering 299(5). p.631-638
Abstract
Particles with functional groups on their surface were immobilized on premade cryogels through covalent bonding to maximize the accessibility of the target molecules to the surface of the particles. The physical properties of the composite cryogel were characterized by SEM, texture analysis and nitrogen adsorption. Different key factors affecting immobilization were also investigated, e.g., recirculation time and the number of functional groups on the premade cryogels. It was found that the physical characteristics of the composite cryogels prepared in this way were very similar to those of the primary matrices, while their binding capacity increased in comparison with conventionally prepared composite cryogels. The binding capacities of... (More)
Particles with functional groups on their surface were immobilized on premade cryogels through covalent bonding to maximize the accessibility of the target molecules to the surface of the particles. The physical properties of the composite cryogel were characterized by SEM, texture analysis and nitrogen adsorption. Different key factors affecting immobilization were also investigated, e.g., recirculation time and the number of functional groups on the premade cryogels. It was found that the physical characteristics of the composite cryogels prepared in this way were very similar to those of the primary matrices, while their binding capacity increased in comparison with conventionally prepared composite cryogels. The binding capacities of two different types of composite cryogel were evaluated by studying the adsorption of small molecules (copper ions and Orange G) and a large molecule (thermostable His-tagged exoglucanase). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
composite, covalent interaction, flow-through immobilization, macroporous hydrogel, post modification
in
Macromolecular Materials and Engineering
volume
299
issue
5
pages
631 - 638
publisher
Wiley-Blackwell
external identifiers
  • wos:000335578400010
  • scopus:84899906599
ISSN
1438-7492
DOI
10.1002/mame.201300358
language
English
LU publication?
yes
id
001450ca-999f-4bae-b14b-3dfec18ee684 (old id 4470592)
date added to LUP
2014-06-19 13:03:14
date last changed
2017-01-01 06:13:39
@article{001450ca-999f-4bae-b14b-3dfec18ee684,
  abstract     = {Particles with functional groups on their surface were immobilized on premade cryogels through covalent bonding to maximize the accessibility of the target molecules to the surface of the particles. The physical properties of the composite cryogel were characterized by SEM, texture analysis and nitrogen adsorption. Different key factors affecting immobilization were also investigated, e.g., recirculation time and the number of functional groups on the premade cryogels. It was found that the physical characteristics of the composite cryogels prepared in this way were very similar to those of the primary matrices, while their binding capacity increased in comparison with conventionally prepared composite cryogels. The binding capacities of two different types of composite cryogel were evaluated by studying the adsorption of small molecules (copper ions and Orange G) and a large molecule (thermostable His-tagged exoglucanase).},
  author       = {Hajizadeh, Solmaz and Mattiasson, Bo and Kirsebom, Harald},
  issn         = {1438-7492},
  keyword      = {composite,covalent interaction,flow-through immobilization,macroporous hydrogel,post modification},
  language     = {eng},
  number       = {5},
  pages        = {631--638},
  publisher    = {Wiley-Blackwell},
  series       = {Macromolecular Materials and Engineering},
  title        = {Flow-Through-Mediated Surface Immobilization of Sub-Micrometre Particles in Monolithic Cryogels},
  url          = {http://dx.doi.org/10.1002/mame.201300358},
  volume       = {299},
  year         = {2014},
}