Genetic associations to germinal centre formation in primary Sjogren's syndrome
(2014) In Annals of the Rheumatic Diseases 73(6). p.1253-1258- Abstract
- Background Primary Sjogren's syndrome (pSS) is an autoimmune rheumatic disease mainly characterised by focal mononuclear cell infiltration in the salivary and lacrimal glands, and by the symptoms xerostomia and keratoconjunctivitis sicca. Germinal centre-like structures (GC) are found in the minor salivary glands of approximately 25% of patients. In this study, we aimed to assess genetic variations in pSS patients with GC-like formations (GC+) compared with patients without such formations (GC-). Methods Minor salivary gland biopsies from Swedish and Norwegian pSS patients (n=320) were evaluated for GC-like formations, identifying 76 GC+ and 244 GC-patients. A panel of 1536 single-nucleotide polymorphisms (SNPs) in 107 genes was genotyped.... (More)
- Background Primary Sjogren's syndrome (pSS) is an autoimmune rheumatic disease mainly characterised by focal mononuclear cell infiltration in the salivary and lacrimal glands, and by the symptoms xerostomia and keratoconjunctivitis sicca. Germinal centre-like structures (GC) are found in the minor salivary glands of approximately 25% of patients. In this study, we aimed to assess genetic variations in pSS patients with GC-like formations (GC+) compared with patients without such formations (GC-). Methods Minor salivary gland biopsies from Swedish and Norwegian pSS patients (n=320) were evaluated for GC-like formations, identifying 76 GC+ and 244 GC-patients. A panel of 1536 single-nucleotide polymorphisms (SNPs) in 107 genes was genotyped. Minor allele frequencies in GC+ and GC- patients were compared using Fisher's exact test, and associations were considered significant when p<4.7x10(-4) and suggestive when p<0.01. Results In this case-only analysis, we identified two SNPs in CCL11 (eotaxin) associated with GC-like structures (p<4.7x10(-4), OR 0.45 and 0.41, respectively). A haplotype of the two minor alleles was associated with GC status with p=2.6x10(-4,) OR 0.40. Suggestive associations (p<0.01) were found in SNPs in the B cell activation and/or GC-formation related genes AICDA, BANK1 and BCL2. Furthermore, SNPs in IL17A, ICA1, PKN1 and SNPs in the NF-kappa B pathway genes CARD8, IKBKE and TANK were found suggestively associated with GC-like structures. Conclusions Our findings suggest that genetic variations may explain why ectopic GC-like structures are present in some pSS patients, and support the hypothesis that GC+ and GC- patients represent distinct disease phenotypes. (Less)
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https://lup.lub.lu.se/record/4482329
- author
- Reksten, Tove Ragna ; Johnsen, Svein Joar Auglaend ; Jonsson, Malin Viktoria ; Omdal, Roald ; Brun, Johan G. ; Theander, Elke LU ; Eriksson, Per ; Wahren-Herlenius, Marie ; Jonsson, Roland and Nordmark, Gunnel
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Annals of the Rheumatic Diseases
- volume
- 73
- issue
- 6
- pages
- 1253 - 1258
- publisher
- BMJ Publishing Group
- external identifiers
-
- wos:000335362100053
- scopus:84899981675
- pmid:23606706
- ISSN
- 1468-2060
- DOI
- 10.1136/annrheumdis-2012-202500
- language
- English
- LU publication?
- yes
- id
- 67b669cf-cf1b-4daf-8cfd-432940567794 (old id 4482329)
- date added to LUP
- 2016-04-01 13:31:46
- date last changed
- 2022-02-19 05:55:42
@article{67b669cf-cf1b-4daf-8cfd-432940567794,
abstract = {{Background Primary Sjogren's syndrome (pSS) is an autoimmune rheumatic disease mainly characterised by focal mononuclear cell infiltration in the salivary and lacrimal glands, and by the symptoms xerostomia and keratoconjunctivitis sicca. Germinal centre-like structures (GC) are found in the minor salivary glands of approximately 25% of patients. In this study, we aimed to assess genetic variations in pSS patients with GC-like formations (GC+) compared with patients without such formations (GC-). Methods Minor salivary gland biopsies from Swedish and Norwegian pSS patients (n=320) were evaluated for GC-like formations, identifying 76 GC+ and 244 GC-patients. A panel of 1536 single-nucleotide polymorphisms (SNPs) in 107 genes was genotyped. Minor allele frequencies in GC+ and GC- patients were compared using Fisher's exact test, and associations were considered significant when p<4.7x10(-4) and suggestive when p<0.01. Results In this case-only analysis, we identified two SNPs in CCL11 (eotaxin) associated with GC-like structures (p<4.7x10(-4), OR 0.45 and 0.41, respectively). A haplotype of the two minor alleles was associated with GC status with p=2.6x10(-4,) OR 0.40. Suggestive associations (p<0.01) were found in SNPs in the B cell activation and/or GC-formation related genes AICDA, BANK1 and BCL2. Furthermore, SNPs in IL17A, ICA1, PKN1 and SNPs in the NF-kappa B pathway genes CARD8, IKBKE and TANK were found suggestively associated with GC-like structures. Conclusions Our findings suggest that genetic variations may explain why ectopic GC-like structures are present in some pSS patients, and support the hypothesis that GC+ and GC- patients represent distinct disease phenotypes.}},
author = {{Reksten, Tove Ragna and Johnsen, Svein Joar Auglaend and Jonsson, Malin Viktoria and Omdal, Roald and Brun, Johan G. and Theander, Elke and Eriksson, Per and Wahren-Herlenius, Marie and Jonsson, Roland and Nordmark, Gunnel}},
issn = {{1468-2060}},
language = {{eng}},
number = {{6}},
pages = {{1253--1258}},
publisher = {{BMJ Publishing Group}},
series = {{Annals of the Rheumatic Diseases}},
title = {{Genetic associations to germinal centre formation in primary Sjogren's syndrome}},
url = {{http://dx.doi.org/10.1136/annrheumdis-2012-202500}},
doi = {{10.1136/annrheumdis-2012-202500}},
volume = {{73}},
year = {{2014}},
}