Advanced

Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells

de Ghellinck, Alexis; Schaller, Hubert; Laux, Valerie; Haertlein, Michael; Sferrazza, Michele; Marechal, Eric; Wacklin, Hanna LU ; Jouhet, Juliette and Fragneto, Giovanna (2014) In PLoS ONE 9(4).
Abstract
Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture... (More)
Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
9
issue
4
publisher
Public Library of Science
external identifiers
  • wos:000335226500003
  • scopus:84899694492
ISSN
1932-6203
DOI
10.1371/journal.pone.0092999
language
English
LU publication?
yes
id
63f8b527-fbca-4bd9-aa79-21ba97030dbf (old id 4487175)
date added to LUP
2014-06-19 14:49:35
date last changed
2017-09-24 04:16:24
@article{63f8b527-fbca-4bd9-aa79-21ba97030dbf,
  abstract     = {Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes.},
  articleno    = {e92999},
  author       = {de Ghellinck, Alexis and Schaller, Hubert and Laux, Valerie and Haertlein, Michael and Sferrazza, Michele and Marechal, Eric and Wacklin, Hanna and Jouhet, Juliette and Fragneto, Giovanna},
  issn         = {1932-6203},
  language     = {eng},
  number       = {4},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells},
  url          = {http://dx.doi.org/10.1371/journal.pone.0092999},
  volume       = {9},
  year         = {2014},
}