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Enhancement of fermentable sugar yield by competitive adsorption of non-enzymatic substances from yeast and cellulase on lignin

Tang, Yong; Lei, Fuhou; Carrasco, Cristhian LU ; Liu, Zuguang; Yu, Hailong and Jiang, Jianxin (2014) In BMC Biotechnology 14.
Abstract
Background: Enhancement of enzymatic digestibility by some supplementations could reduce enzyme loading and cost, which is still too high to realize economical production of lignocellulosic biofuels. A recent study indicates that yeast hydrolysates (YH) have improved the efficiency of cellulases on digestibility of furfural residues (FR). In the current work, the components of YH were separated by centrifugation and size exclusion chromatography and finally characterized in order to better understand this positive effect. Results: A 60.8% of nitrogen of yeast cells was remained in the slurry (YHS) after hydrothermal treatment. In the supernatant of YH (YHL), substances of high molecular weight were identified as proteins and other... (More)
Background: Enhancement of enzymatic digestibility by some supplementations could reduce enzyme loading and cost, which is still too high to realize economical production of lignocellulosic biofuels. A recent study indicates that yeast hydrolysates (YH) have improved the efficiency of cellulases on digestibility of furfural residues (FR). In the current work, the components of YH were separated by centrifugation and size exclusion chromatography and finally characterized in order to better understand this positive effect. Results: A 60.8% of nitrogen of yeast cells was remained in the slurry (YHS) after hydrothermal treatment. In the supernatant of YH (YHL), substances of high molecular weight were identified as proteins and other UV-absorbing compounds, which showed close molecular weight to components of cellulases. Those substances attributed to a synergetic positive effect on enzymatic hydrolysis of FR. The fraction of YHL ranged from 1.19 to 2.19 mL (elution volume) contained over 50% of proteins in YHL and had the best performance in stimulating the release of glucose. Experiment results proved the adsorption of proteins in YHL on lignin. Conclusions: Supplementation of cellulases with YH enhances enzymatic digestibility of FR mainly by a competitive adsorption of non-enzymatic substances on lignin. The molecular weight of these substances has a significant impact on their performance. Different strategies can be used for a good utilization of yeast cells in terms of biorefinery concept. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cellulases, Enzymatic hydrolysis, Yeast hydrolysate, Competitive, adsorption
in
BMC Biotechnology
volume
14
publisher
BioMed Central
external identifiers
  • wos:000334882300001
  • scopus:84898926942
ISSN
1472-6750
DOI
10.1186/1472-6750-14-21
language
English
LU publication?
yes
id
98ab6b17-3536-4c65-881a-eaaacc43a9ff (old id 4495783)
date added to LUP
2014-06-23 13:09:19
date last changed
2017-06-04 03:54:54
@article{98ab6b17-3536-4c65-881a-eaaacc43a9ff,
  abstract     = {Background: Enhancement of enzymatic digestibility by some supplementations could reduce enzyme loading and cost, which is still too high to realize economical production of lignocellulosic biofuels. A recent study indicates that yeast hydrolysates (YH) have improved the efficiency of cellulases on digestibility of furfural residues (FR). In the current work, the components of YH were separated by centrifugation and size exclusion chromatography and finally characterized in order to better understand this positive effect. Results: A 60.8% of nitrogen of yeast cells was remained in the slurry (YHS) after hydrothermal treatment. In the supernatant of YH (YHL), substances of high molecular weight were identified as proteins and other UV-absorbing compounds, which showed close molecular weight to components of cellulases. Those substances attributed to a synergetic positive effect on enzymatic hydrolysis of FR. The fraction of YHL ranged from 1.19 to 2.19 mL (elution volume) contained over 50% of proteins in YHL and had the best performance in stimulating the release of glucose. Experiment results proved the adsorption of proteins in YHL on lignin. Conclusions: Supplementation of cellulases with YH enhances enzymatic digestibility of FR mainly by a competitive adsorption of non-enzymatic substances on lignin. The molecular weight of these substances has a significant impact on their performance. Different strategies can be used for a good utilization of yeast cells in terms of biorefinery concept.},
  articleno    = {21},
  author       = {Tang, Yong and Lei, Fuhou and Carrasco, Cristhian and Liu, Zuguang and Yu, Hailong and Jiang, Jianxin},
  issn         = {1472-6750},
  keyword      = {Cellulases,Enzymatic hydrolysis,Yeast hydrolysate,Competitive,adsorption},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Biotechnology},
  title        = {Enhancement of fermentable sugar yield by competitive adsorption of non-enzymatic substances from yeast and cellulase on lignin},
  url          = {http://dx.doi.org/10.1186/1472-6750-14-21},
  volume       = {14},
  year         = {2014},
}