Biochemical and structural characterisation of membrane-containing icosahedral dsDNA bacteriophages infecting thermophilic Thermus thermophilus
(2008) In Virology 379(1). p.10-19- Abstract
Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the Gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal... (More)
Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the Gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal membrane. The capsid architecture of P23-77 is most similar to that of the archaeal virus SH1. These findings further complicate the grouping of icosahedrally-symmetric viruses containing an inner membrane. We propose a single superfamily or order with members in several viral families.
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- author
- Jaatinen, S. T. ; Happonen, L. J. LU ; Laurinmäki, P. ; Butcher, S. J. and Bamford, D. H.
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- keywords
- Bacteriophage, Membrane-containing, P23-65H, P23-72, P23-77, Thermophilic, Thermus, Thermus thermophilus
- in
- Virology
- volume
- 379
- issue
- 1
- pages
- 10 - 19
- publisher
- Elsevier
- external identifiers
-
- pmid:18657283
- scopus:48949087957
- ISSN
- 0042-6822
- DOI
- 10.1016/j.virol.2008.06.023
- language
- English
- LU publication?
- no
- additional info
- Funding Information: The technical assistance of Anna Latva-Käyrä and Heini Hyvönen is gratefully acknowledged. Harri Jäälinoja and Jani Seitsonen are acknowledged for the useful discussions. We thank the Electron Microscopy Unit of the Institute of Biotechnology, University of Helsinki for providing microscopy facilities. This study was supported (to S.J.B and D.H.B) from the Finnish Center of Excellence Program [2006–2011] grant 1213467. Further funding was obtained from the Academy of Finland [grant 1210253] to D.H.B and grant 1112244 to S. J. B. As part of the European Science Foundation EUROCORES Programme Euro-SCOPE, the work is also supported by funds from the European Commission's Sixth Framework Programme under contract ERAS-CT-2003-980409. The Helsinki Graduate School of Biotechnology and Molecular Biology is acknowledged for funding of S.T.J.
- id
- 44f1b7ae-e8c9-4605-b9b7-721c34853afe
- date added to LUP
- 2021-11-22 05:36:58
- date last changed
- 2024-01-05 20:52:38
@article{44f1b7ae-e8c9-4605-b9b7-721c34853afe,
abstract = {{<p>Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the Gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal membrane. The capsid architecture of P23-77 is most similar to that of the archaeal virus SH1. These findings further complicate the grouping of icosahedrally-symmetric viruses containing an inner membrane. We propose a single superfamily or order with members in several viral families.</p>}},
author = {{Jaatinen, S. T. and Happonen, L. J. and Laurinmäki, P. and Butcher, S. J. and Bamford, D. H.}},
issn = {{0042-6822}},
keywords = {{Bacteriophage; Membrane-containing; P23-65H; P23-72; P23-77; Thermophilic; Thermus; Thermus thermophilus}},
language = {{eng}},
number = {{1}},
pages = {{10--19}},
publisher = {{Elsevier}},
series = {{Virology}},
title = {{Biochemical and structural characterisation of membrane-containing icosahedral dsDNA bacteriophages infecting thermophilic Thermus thermophilus}},
url = {{http://dx.doi.org/10.1016/j.virol.2008.06.023}},
doi = {{10.1016/j.virol.2008.06.023}},
volume = {{379}},
year = {{2008}},
}