Generation of miniaturized planar ecombinant antibody arrays using a microcantilever-based printer.
(2014) In Nanotechnology 25(27).- Abstract
- Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-consisting of an array of micromachined silicon cantilevers with integrated microfluidic channels-to produce miniaturized, multiplexed, and high-density planar recombinant antibody arrays for protein expression profiling which targets crude, directly labelled serum. The results demonstrated that 16-plex recombinant antibody arrays could be... (More)
- Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-consisting of an array of micromachined silicon cantilevers with integrated microfluidic channels-to produce miniaturized, multiplexed, and high-density planar recombinant antibody arrays for protein expression profiling which targets crude, directly labelled serum. The results demonstrated that 16-plex recombinant antibody arrays could be produced-based on miniaturized spot features (78.5 um(2), Ø 10 μm) at a 7-125-times increased spot density (250 000 spots cm(-2)), interfaced with a fluorescent-based read-out. This prototype platform was found to display adequate reproducibility (spot-to-spot) and an assay sensitivity in the pM range. The feasibility of the array platform for serum protein profiling was outlined. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4526890
- author
- Petersson, Linn LU ; Berthet Duroure, Nathalie ; Auger, Angèle ; Dexlin Mellby, Linda LU ; Borrebaeck, Carl LU ; Ait Ikhlef, Ali and Wingren, Christer LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nanotechnology
- volume
- 25
- issue
- 27
- article number
- 275104
- publisher
- IOP Publishing
- external identifiers
-
- pmid:24960426
- wos:000338715800004
- scopus:84903289036
- pmid:24960426
- ISSN
- 0957-4484
- DOI
- 10.1088/0957-4484/25/27/275104
- language
- English
- LU publication?
- yes
- id
- f2043619-78ce-4492-9590-260bd22b0232 (old id 4526890)
- date added to LUP
- 2016-04-01 11:10:04
- date last changed
- 2022-01-26 05:56:03
@article{f2043619-78ce-4492-9590-260bd22b0232, abstract = {{Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-consisting of an array of micromachined silicon cantilevers with integrated microfluidic channels-to produce miniaturized, multiplexed, and high-density planar recombinant antibody arrays for protein expression profiling which targets crude, directly labelled serum. The results demonstrated that 16-plex recombinant antibody arrays could be produced-based on miniaturized spot features (78.5 um(2), Ø 10 μm) at a 7-125-times increased spot density (250 000 spots cm(-2)), interfaced with a fluorescent-based read-out. This prototype platform was found to display adequate reproducibility (spot-to-spot) and an assay sensitivity in the pM range. The feasibility of the array platform for serum protein profiling was outlined.}}, author = {{Petersson, Linn and Berthet Duroure, Nathalie and Auger, Angèle and Dexlin Mellby, Linda and Borrebaeck, Carl and Ait Ikhlef, Ali and Wingren, Christer}}, issn = {{0957-4484}}, language = {{eng}}, number = {{27}}, publisher = {{IOP Publishing}}, series = {{Nanotechnology}}, title = {{Generation of miniaturized planar ecombinant antibody arrays using a microcantilever-based printer.}}, url = {{http://dx.doi.org/10.1088/0957-4484/25/27/275104}}, doi = {{10.1088/0957-4484/25/27/275104}}, volume = {{25}}, year = {{2014}}, }