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Generation of miniaturized planar ecombinant antibody arrays using a microcantilever-based printer.

Petersson, Linn LU ; Berthet Duroure, Nathalie; Auger, Angèle; Dexlin Mellby, Linda LU ; Borrebaeck, Carl LU ; Ait Ikhlef, Ali and Wingren, Christer LU (2014) In Nanotechnology 25(27).
Abstract
Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-consisting of an array of micromachined silicon cantilevers with integrated microfluidic channels-to produce miniaturized, multiplexed, and high-density planar recombinant antibody arrays for protein expression profiling which targets crude, directly labelled serum. The results demonstrated that 16-plex recombinant antibody arrays could be... (More)
Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-consisting of an array of micromachined silicon cantilevers with integrated microfluidic channels-to produce miniaturized, multiplexed, and high-density planar recombinant antibody arrays for protein expression profiling which targets crude, directly labelled serum. The results demonstrated that 16-plex recombinant antibody arrays could be produced-based on miniaturized spot features (78.5 um(2), Ø 10 μm) at a 7-125-times increased spot density (250 000 spots cm(-2)), interfaced with a fluorescent-based read-out. This prototype platform was found to display adequate reproducibility (spot-to-spot) and an assay sensitivity in the pM range. The feasibility of the array platform for serum protein profiling was outlined. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nanotechnology
volume
25
issue
27
publisher
IOP Publishing
external identifiers
  • pmid:24960426
  • wos:000338715800004
  • scopus:84903289036
ISSN
0957-4484
DOI
10.1088/0957-4484/25/27/275104
language
English
LU publication?
yes
id
f2043619-78ce-4492-9590-260bd22b0232 (old id 4526890)
date added to LUP
2014-07-24 11:15:16
date last changed
2017-02-12 03:20:42
@article{f2043619-78ce-4492-9590-260bd22b0232,
  abstract     = {Miniaturized (Ø 10 μm), multiplexed (>5-plex), and high-density (>100 000 spots cm(-2)) antibody arrays will play a key role in generating protein expression profiles in health and disease. However, producing such antibody arrays is challenging, and it is the type and range of available spotters which set the stage. This pilot study explored the use of a novel microspotting tool, Bioplume(TM)-consisting of an array of micromachined silicon cantilevers with integrated microfluidic channels-to produce miniaturized, multiplexed, and high-density planar recombinant antibody arrays for protein expression profiling which targets crude, directly labelled serum. The results demonstrated that 16-plex recombinant antibody arrays could be produced-based on miniaturized spot features (78.5 um(2), Ø 10 μm) at a 7-125-times increased spot density (250 000 spots cm(-2)), interfaced with a fluorescent-based read-out. This prototype platform was found to display adequate reproducibility (spot-to-spot) and an assay sensitivity in the pM range. The feasibility of the array platform for serum protein profiling was outlined.},
  articleno    = {275104},
  author       = {Petersson, Linn and Berthet Duroure, Nathalie and Auger, Angèle and Dexlin Mellby, Linda and Borrebaeck, Carl and Ait Ikhlef, Ali and Wingren, Christer},
  issn         = {0957-4484},
  language     = {eng},
  number       = {27},
  publisher    = {IOP Publishing},
  series       = {Nanotechnology},
  title        = {Generation of miniaturized planar ecombinant antibody arrays using a microcantilever-based printer.},
  url          = {http://dx.doi.org/10.1088/0957-4484/25/27/275104},
  volume       = {25},
  year         = {2014},
}