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See-saw rocking: an in vitro model for mechanotransduction research.

Tucker, R P; Henningsson, Per LU ; Franklin, S L; Chen, D; Ventikos, Y; Bomphrey, R J and Thompson, M S (2014) In Journal of the Royal Society Interface 11(97).
Abstract
In vitro mechanotransduction studies, uncovering the basic science of the response of cells to mechanical forces, are essential for progress in tissue engineering and its clinical application. Many varying investigations have described a multitude of cell responses; however, as the precise nature and magnitude of the stresses applied are infrequently reported and rarely validated, the experiments are often not comparable, limiting research progress. This paper provides physical and biological validation of a widely available fluid stimulation device, a see-saw rocker, as an in vitro model for cyclic fluid shear stress mechanotransduction. This allows linkage between precisely characterized stimuli and cell monolayer response in a... (More)
In vitro mechanotransduction studies, uncovering the basic science of the response of cells to mechanical forces, are essential for progress in tissue engineering and its clinical application. Many varying investigations have described a multitude of cell responses; however, as the precise nature and magnitude of the stresses applied are infrequently reported and rarely validated, the experiments are often not comparable, limiting research progress. This paper provides physical and biological validation of a widely available fluid stimulation device, a see-saw rocker, as an in vitro model for cyclic fluid shear stress mechanotransduction. This allows linkage between precisely characterized stimuli and cell monolayer response in a convenient six-well plate format. Models of one well were discretized and analysed extensively using computational fluid dynamics to generate convergent, stable and consistent predictions of the cyclic fluid velocity vectors at a rocking frequency of 0.5 Hz, accounting for the free surface. Validation was provided by comparison with flow velocities measured experimentally using particle image velocimetry. Qualitative flow behaviour was matched and quantitative analysis showed agreement at representative locations and time points. Maximum shear stress of 0.22 Pa was estimated near the well edge, and time-average shear stress ranged between 0.029 and 0.068 Pa. Human tenocytes stimulated using the system showed significant increases in collagen and GAG secretion at 2 and 7 day time points. This in vitro model for mechanotransduction provides a versatile, flexible and inexpensive method for the fluid shear stress impact on biological cells to be studied. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Journal of the Royal Society Interface
volume
11
issue
97
publisher
Royal Society
external identifiers
  • pmid:24898022
  • wos:000338436200012
  • scopus:84903648185
ISSN
1742-5662
DOI
10.1098/rsif.2014.0330
language
English
LU publication?
yes
id
aa9210da-a7fe-44ab-ae9f-94fac1520f54 (old id 4529251)
date added to LUP
2014-08-12 09:00:08
date last changed
2017-08-27 03:20:27
@article{aa9210da-a7fe-44ab-ae9f-94fac1520f54,
  abstract     = {In vitro mechanotransduction studies, uncovering the basic science of the response of cells to mechanical forces, are essential for progress in tissue engineering and its clinical application. Many varying investigations have described a multitude of cell responses; however, as the precise nature and magnitude of the stresses applied are infrequently reported and rarely validated, the experiments are often not comparable, limiting research progress. This paper provides physical and biological validation of a widely available fluid stimulation device, a see-saw rocker, as an in vitro model for cyclic fluid shear stress mechanotransduction. This allows linkage between precisely characterized stimuli and cell monolayer response in a convenient six-well plate format. Models of one well were discretized and analysed extensively using computational fluid dynamics to generate convergent, stable and consistent predictions of the cyclic fluid velocity vectors at a rocking frequency of 0.5 Hz, accounting for the free surface. Validation was provided by comparison with flow velocities measured experimentally using particle image velocimetry. Qualitative flow behaviour was matched and quantitative analysis showed agreement at representative locations and time points. Maximum shear stress of 0.22 Pa was estimated near the well edge, and time-average shear stress ranged between 0.029 and 0.068 Pa. Human tenocytes stimulated using the system showed significant increases in collagen and GAG secretion at 2 and 7 day time points. This in vitro model for mechanotransduction provides a versatile, flexible and inexpensive method for the fluid shear stress impact on biological cells to be studied.},
  articleno    = {20140330},
  author       = {Tucker, R P and Henningsson, Per and Franklin, S L and Chen, D and Ventikos, Y and Bomphrey, R J and Thompson, M S},
  issn         = {1742-5662},
  language     = {eng},
  number       = {97},
  publisher    = {Royal Society},
  series       = {Journal of the Royal Society Interface},
  title        = {See-saw rocking: an in vitro model for mechanotransduction research.},
  url          = {http://dx.doi.org/10.1098/rsif.2014.0330},
  volume       = {11},
  year         = {2014},
}