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Retinal Function and Morphology in the Rabbit Eye after Intravitreal Injection of the TNF Alpha Inhibitor Adalimumab.

Cardiakidis Myers, Anna LU ; Ghosh, Fredrik LU ; Andréasson, Sten LU and Ponjavic, Vesna LU (2014) In Current Eye Research 39(11). p.1106-1116
Abstract
Abstract Aim: To study the effects of the tumor necrosis factor alpha inhibitor adalimumab on rabbit retina after injection into the vitreous body. Methods: Forty-eight rabbits of mixed strain (9-12 months old, weighing ≈ 3.5 kg) were randomized into four groups. Adalimumab was injected at one of two concentrations (1.25 mg or 2.5 mg) into the eyes of two groups, and balanced salt solution into the eyes of the third group. The fourth group acted as controls. Full-field electroretinography (ffERG) was performed before injection and 1 and 6 weeks post-injection. At 6 weeks post-injection the rabbits were euthanized and the sectioned retinas were studied. Retinal histology was studied with hematoxylin-eosin staining. Immunohistochemical... (More)
Abstract Aim: To study the effects of the tumor necrosis factor alpha inhibitor adalimumab on rabbit retina after injection into the vitreous body. Methods: Forty-eight rabbits of mixed strain (9-12 months old, weighing ≈ 3.5 kg) were randomized into four groups. Adalimumab was injected at one of two concentrations (1.25 mg or 2.5 mg) into the eyes of two groups, and balanced salt solution into the eyes of the third group. The fourth group acted as controls. Full-field electroretinography (ffERG) was performed before injection and 1 and 6 weeks post-injection. At 6 weeks post-injection the rabbits were euthanized and the sectioned retinas were studied. Retinal histology was studied with hematoxylin-eosin staining. Immunohistochemical analysis was performed on rods, cones, rod bipolar cells, horizontal cells, amacrine cells and Müller cells. Results: No significant difference in ffERG amplitudes or implicit times was observed between the four groups at any time point. Histological and immunohistochemical findings were similar in all groups. Conclusions: Injection of adalimumab into the vitreous body of healthy rabbits, at doses up to 2.5 mg, does not appear to be toxic to the rabbit retina. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Eye Research
volume
39
issue
11
pages
1106 - 1116
publisher
Taylor & Francis
external identifiers
  • pmid:24897597
  • wos:000344003900006
  • scopus:84911970080
ISSN
0271-3683
DOI
10.3109/02713683.2014.898309
language
English
LU publication?
yes
id
662efa2f-a1d5-4ffb-8246-e54dd6b2d95e (old id 4529261)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24897597?dopt=Abstract
date added to LUP
2014-07-04 14:47:18
date last changed
2017-09-03 03:26:43
@article{662efa2f-a1d5-4ffb-8246-e54dd6b2d95e,
  abstract     = {Abstract Aim: To study the effects of the tumor necrosis factor alpha inhibitor adalimumab on rabbit retina after injection into the vitreous body. Methods: Forty-eight rabbits of mixed strain (9-12 months old, weighing ≈ 3.5 kg) were randomized into four groups. Adalimumab was injected at one of two concentrations (1.25 mg or 2.5 mg) into the eyes of two groups, and balanced salt solution into the eyes of the third group. The fourth group acted as controls. Full-field electroretinography (ffERG) was performed before injection and 1 and 6 weeks post-injection. At 6 weeks post-injection the rabbits were euthanized and the sectioned retinas were studied. Retinal histology was studied with hematoxylin-eosin staining. Immunohistochemical analysis was performed on rods, cones, rod bipolar cells, horizontal cells, amacrine cells and Müller cells. Results: No significant difference in ffERG amplitudes or implicit times was observed between the four groups at any time point. Histological and immunohistochemical findings were similar in all groups. Conclusions: Injection of adalimumab into the vitreous body of healthy rabbits, at doses up to 2.5 mg, does not appear to be toxic to the rabbit retina.},
  author       = {Cardiakidis Myers, Anna and Ghosh, Fredrik and Andréasson, Sten and Ponjavic, Vesna},
  issn         = {0271-3683},
  language     = {eng},
  number       = {11},
  pages        = {1106--1116},
  publisher    = {Taylor & Francis},
  series       = {Current Eye Research},
  title        = {Retinal Function and Morphology in the Rabbit Eye after Intravitreal Injection of the TNF Alpha Inhibitor Adalimumab.},
  url          = {http://dx.doi.org/10.3109/02713683.2014.898309},
  volume       = {39},
  year         = {2014},
}