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Adenovirus assembly is impaired by BMI1-related histone deacetylase activity.

Na, Manli LU ; Chen, Dongfeng LU ; Holmqvist, Bo LU ; Ran, Liang; Jin, Jie LU ; Rebetz, Johan LU and Fan, Xiaolong LU (2014) In Virology 456(Apr 17). p.227-237
Abstract
Polycomb ring finger oncogene BMI1 (B cell-specific Moloney murine leukemia virus integration site 1) plays a critical role in development of several types of cancers. Here, we report an inverse relationship between levels of BMI1 expression and adenovirus (Ad) progeny production. Enforced BMI1 expression in A549 cells impaired Ad progeny production. In contrast, knocking-down of endogenous BMI1 expression enhanced progeny production of a conditionally replicating Ad and wild-type Ad5 and Ad11p. Ad vectors overexpressing BMI1 were not impaired in the replication of progeny genomes and in the expression of E1A and Ad structural proteins. However, 293 cells infected by Ad vector overexpressing BMI1 contained a large proportion of... (More)
Polycomb ring finger oncogene BMI1 (B cell-specific Moloney murine leukemia virus integration site 1) plays a critical role in development of several types of cancers. Here, we report an inverse relationship between levels of BMI1 expression and adenovirus (Ad) progeny production. Enforced BMI1 expression in A549 cells impaired Ad progeny production. In contrast, knocking-down of endogenous BMI1 expression enhanced progeny production of a conditionally replicating Ad and wild-type Ad5 and Ad11p. Ad vectors overexpressing BMI1 were not impaired in the replication of progeny genomes and in the expression of E1A and Ad structural proteins. However, 293 cells infected by Ad vector overexpressing BMI1 contained a large proportion of morphologically irregular Ad particles. This effect was reversed in 293 cells pre-treated with the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) in parallel with the production of infectious Ad particles. Our findings suggest an inhibitory role of BMI1 in Ad morphogenesis that can be implied in Ad tropism and Ad-mediated cancer therapy. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Virology
volume
456
issue
Apr 17
pages
227 - 237
publisher
Elsevier
external identifiers
  • pmid:24889242
  • wos:000337258600023
  • scopus:84898857298
ISSN
1096-0341
DOI
10.1016/j.virol.2014.03.025
language
English
LU publication?
yes
id
6fb786f7-7a3c-4ab8-ba64-48152e9d93ae (old id 4529363)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24889242?dopt=Abstract
date added to LUP
2014-07-04 14:02:30
date last changed
2017-01-01 03:23:06
@article{6fb786f7-7a3c-4ab8-ba64-48152e9d93ae,
  abstract     = {Polycomb ring finger oncogene BMI1 (B cell-specific Moloney murine leukemia virus integration site 1) plays a critical role in development of several types of cancers. Here, we report an inverse relationship between levels of BMI1 expression and adenovirus (Ad) progeny production. Enforced BMI1 expression in A549 cells impaired Ad progeny production. In contrast, knocking-down of endogenous BMI1 expression enhanced progeny production of a conditionally replicating Ad and wild-type Ad5 and Ad11p. Ad vectors overexpressing BMI1 were not impaired in the replication of progeny genomes and in the expression of E1A and Ad structural proteins. However, 293 cells infected by Ad vector overexpressing BMI1 contained a large proportion of morphologically irregular Ad particles. This effect was reversed in 293 cells pre-treated with the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) in parallel with the production of infectious Ad particles. Our findings suggest an inhibitory role of BMI1 in Ad morphogenesis that can be implied in Ad tropism and Ad-mediated cancer therapy.},
  author       = {Na, Manli and Chen, Dongfeng and Holmqvist, Bo and Ran, Liang and Jin, Jie and Rebetz, Johan and Fan, Xiaolong},
  issn         = {1096-0341},
  language     = {eng},
  number       = {Apr 17},
  pages        = {227--237},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Adenovirus assembly is impaired by BMI1-related histone deacetylase activity.},
  url          = {http://dx.doi.org/10.1016/j.virol.2014.03.025},
  volume       = {456},
  year         = {2014},
}