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Localization of Sunitinib in in vivo Animal and in vitro Experimental Models by MALDI Mass Spectrometry Imaging

James, Connell; Sugihara, Yutaka LU ; Török, Szilvia; Döme, Balázs; Fehniger, Thomas LU ; Marko-Varga, György LU and Végvári, Ákos LU (2015) In Analytical and Bioanalytical Chemistry 407(8). p.2245-2253
Abstract
The spatial distribution of an anti-cancer drug and its intended target within a tumor plays a major role on determining how effective the drug can be at tackling the tumor. This study provides data regarding the lateral distribution of sunitinib, an oral antiangiogenic receptor tyrosine kinase inhibitor using an in vitro animal model as well as an in vitro experimental model that involved deposition of a solution of sunitinib onto tissue sections. All tumor sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging and compared with subsequent histology staining. Six tumors at four different time points after commencement of in vivo sunitinib treatment were examined to observe the patterns of drug... (More)
The spatial distribution of an anti-cancer drug and its intended target within a tumor plays a major role on determining how effective the drug can be at tackling the tumor. This study provides data regarding the lateral distribution of sunitinib, an oral antiangiogenic receptor tyrosine kinase inhibitor using an in vitro animal model as well as an in vitro experimental model that involved deposition of a solution of sunitinib onto tissue sections. All tumor sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging and compared with subsequent histology staining. Six tumors at four different time points after commencement of in vivo sunitinib treatment were examined to observe the patterns of drug uptake. The levels of sunitinib present in in vivo treated tumor sections increased continuously until day seven but a decrease was observed at day 10. Furthermore, the in vitro experimental model was adjustable to produce a drug level similar to that obtained in the in vivo model experiments. The distribution of sunitinib in tissue sections treated in vitro appeared to agree with the histological structure of tumors suggesting that this approach may be useful for testing drug update. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Mass spectrometry, MALDI, MALDI-MSI, In vivo models, Drug, Sunitinib, Colorectal adenocarcinoma
in
Analytical and Bioanalytical Chemistry
volume
407
issue
8
pages
2245 - 2253
publisher
Springer
external identifiers
  • pmid:25424181
  • wos:000351195800022
  • scopus:84952719869
  • scopus:85027937155
ISSN
1618-2642
DOI
10.1007/s00216-014-8350-2
language
English
LU publication?
yes
id
e03ff15c-886e-4cf7-8ec6-63f97bd30733 (old id 4532817)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25424181?dopt=Abstract
date added to LUP
2014-11-21 12:59:53
date last changed
2017-09-25 12:23:34
@article{e03ff15c-886e-4cf7-8ec6-63f97bd30733,
  abstract     = {The spatial distribution of an anti-cancer drug and its intended target within a tumor plays a major role on determining how effective the drug can be at tackling the tumor. This study provides data regarding the lateral distribution of sunitinib, an oral antiangiogenic receptor tyrosine kinase inhibitor using an in vitro animal model as well as an in vitro experimental model that involved deposition of a solution of sunitinib onto tissue sections. All tumor sections were analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging and compared with subsequent histology staining. Six tumors at four different time points after commencement of in vivo sunitinib treatment were examined to observe the patterns of drug uptake. The levels of sunitinib present in in vivo treated tumor sections increased continuously until day seven but a decrease was observed at day 10. Furthermore, the in vitro experimental model was adjustable to produce a drug level similar to that obtained in the in vivo model experiments. The distribution of sunitinib in tissue sections treated in vitro appeared to agree with the histological structure of tumors suggesting that this approach may be useful for testing drug update.},
  author       = {James, Connell and Sugihara, Yutaka and Török, Szilvia and Döme, Balázs and Fehniger, Thomas and Marko-Varga, György and Végvári, Ákos},
  issn         = {1618-2642},
  keyword      = {Mass spectrometry,MALDI,MALDI-MSI,In vivo models,Drug,Sunitinib,Colorectal adenocarcinoma},
  language     = {eng},
  number       = {8},
  pages        = {2245--2253},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Localization of Sunitinib in in vivo Animal and in vitro Experimental Models by MALDI Mass Spectrometry Imaging},
  url          = {http://dx.doi.org/10.1007/s00216-014-8350-2},
  volume       = {407},
  year         = {2015},
}