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In vivo distribution of tiotropium in a rodent model utilizing AP-SMALDI mass spectrometry imaging

Végvári, Ákos LU ; Fehniger, Thomas LU ; Dahlbäck, Magnus; Marko-Varga, György LU and Strupat, Kerstin (2016) In Current Analytical Chemistry 13(3). p.182-186
Abstract
Background: Matrix-assisted laser desorption/ionization mass spectrometry imaging has proved to be a powerful tool in localization of endogenous molecules in thin tissue sections. Distributions of drug compounds following administration in experimental animal models can be spatially detected and superimposed upon histological images thereby identifying the tissue compartments involved in drug uptake, transfer and metabolism.

Methods: A new instrumental setup consisting of a Thermo Scientific Q Exactive mass spectrometer and an AP SMALDI10 ion source was used for imaging data acquisition at 10-µm lateral resolution to provide details of the distribution of a bronchodilator, tiotropium, in a rodent model.

Results: The... (More)
Background: Matrix-assisted laser desorption/ionization mass spectrometry imaging has proved to be a powerful tool in localization of endogenous molecules in thin tissue sections. Distributions of drug compounds following administration in experimental animal models can be spatially detected and superimposed upon histological images thereby identifying the tissue compartments involved in drug uptake, transfer and metabolism.

Methods: A new instrumental setup consisting of a Thermo Scientific Q Exactive mass spectrometer and an AP SMALDI10 ion source was used for imaging data acquisition at 10-µm lateral resolution to provide details of the distribution of a bronchodilator, tiotropium, in a rodent model.

Results: The extracted ion maps of tiotropium showed higher signal intensities in bronchioles compared to lung parenchyma, while the drug was absent in lymphoid nodes and blood vessels. The enhanced mass spectrometric image quality provided refined spatial distribution in comparison with data obtained on a Thermo Scientific MALDI LTQ Orbitrap XL mass spectrometer obtained at 30, 80 and 150 µm lateral resolution.

Conclusion: The high spatial resolution images of administrated tiotropium in rat lung obtained by the AP SMALDI10 ion source have readily improved the localization of this drug, highlighting cellular level distributions from the airways to the parenchyma. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Atmospheric pressure scanning matrix-assisted laser desorption/ionization, Mass spectrometry imaging, Chronic obstructive pulmonary disease, Tiotropium, In vivo animal model
in
Current Analytical Chemistry
volume
13
issue
3
pages
5 pages
publisher
Bentham Science Publishers
external identifiers
  • scopus:85020240504
  • wos:000402466200001
ISSN
1875-6727
DOI
10.2174/1573411012666160211235640
language
English
LU publication?
yes
id
739efd90-59fd-44e7-a0c8-61dcc0d9217f (old id 4532821)
alternative location
http://benthamscience.com/journals/current-analytical-chemistry/article/139425/
date added to LUP
2016-03-01 11:34:58
date last changed
2017-09-18 11:28:19
@article{739efd90-59fd-44e7-a0c8-61dcc0d9217f,
  abstract     = {Background: Matrix-assisted laser desorption/ionization mass spectrometry imaging has proved to be a powerful tool in localization of endogenous molecules in thin tissue sections. Distributions of drug compounds following administration in experimental animal models can be spatially detected and superimposed upon histological images thereby identifying the tissue compartments involved in drug uptake, transfer and metabolism. <br/><br>
Methods: A new instrumental setup consisting of a Thermo Scientific Q Exactive mass spectrometer and an AP SMALDI10 ion source was used for imaging data acquisition at 10-µm lateral resolution to provide details of the distribution of a bronchodilator, tiotropium, in a rodent model. <br/><br>
Results: The extracted ion maps of tiotropium showed higher signal intensities in bronchioles compared to lung parenchyma, while the drug was absent in lymphoid nodes and blood vessels. The enhanced mass spectrometric image quality provided refined spatial distribution in comparison with data obtained on a Thermo Scientific MALDI LTQ Orbitrap XL mass spectrometer obtained at 30, 80 and 150 µm lateral resolution. <br/><br>
Conclusion: The high spatial resolution images of administrated tiotropium in rat lung obtained by the AP SMALDI10 ion source have readily improved the localization of this drug, highlighting cellular level distributions from the airways to the parenchyma.},
  author       = {Végvári, Ákos and Fehniger, Thomas and Dahlbäck, Magnus and Marko-Varga, György and Strupat, Kerstin},
  issn         = {1875-6727},
  keyword      = {Atmospheric pressure scanning matrix-assisted laser desorption/ionization,Mass spectrometry imaging,Chronic obstructive pulmonary disease,Tiotropium,In vivo animal model},
  language     = {eng},
  number       = {3},
  pages        = {182--186},
  publisher    = {Bentham Science Publishers},
  series       = {Current Analytical Chemistry},
  title        = {In vivo distribution of tiotropium in a rodent model utilizing AP-SMALDI mass spectrometry imaging},
  url          = {http://dx.doi.org/10.2174/1573411012666160211235640},
  volume       = {13},
  year         = {2016},
}