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Indigenous enteric eosinophils control DCs to initiate a primary Th2 immune response in vivo.

Chu, Derek K; Jimenez-Saiz, Rodrigo; Verschoor, Christopher P; Walker, Tina D; Goncharova, Susanna; Llop-Guevara, Alba; Shen, Pamela; Gordon, Melissa E; Barra, Nicole G and Bassett, Jennifer D, et al. (2014) In Journal of Experimental Medicine 211(8). p.1657-1672
Abstract
Eosinophils natively inhabit the small intestine, but a functional role for them there has remained elusive. Here, we show that eosinophil-deficient mice were protected from induction of Th2-mediated peanut food allergy and anaphylaxis, and Th2 priming was restored by reconstitution with il4(+/+) or il4(-/-) eosinophils. Eosinophils controlled CD103(+) dendritic cell (DC) activation and migration from the intestine to draining lymph nodes, events necessary for Th2 priming. Eosinophil activation in vitro and in vivo led to degranulation of eosinophil peroxidase, a granule protein whose enzymatic activity promoted DC activation in mice and humans in vitro, and intestinal and extraintestinal mouse DC activation and mobilization to lymph nodes... (More)
Eosinophils natively inhabit the small intestine, but a functional role for them there has remained elusive. Here, we show that eosinophil-deficient mice were protected from induction of Th2-mediated peanut food allergy and anaphylaxis, and Th2 priming was restored by reconstitution with il4(+/+) or il4(-/-) eosinophils. Eosinophils controlled CD103(+) dendritic cell (DC) activation and migration from the intestine to draining lymph nodes, events necessary for Th2 priming. Eosinophil activation in vitro and in vivo led to degranulation of eosinophil peroxidase, a granule protein whose enzymatic activity promoted DC activation in mice and humans in vitro, and intestinal and extraintestinal mouse DC activation and mobilization to lymph nodes in vivo. Further, eosinophil peroxidase enhanced responses to ovalbumin seen after immunization. Thus, eosinophils can be critical contributors to the intestinal immune system, and granule-mediated shaping of DC responses can promote both intestinal and extraintestinal adaptive immunity. (Less)
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Journal of Experimental Medicine
volume
211
issue
8
pages
1657 - 1672
publisher
Rockefeller University Press
external identifiers
  • pmid:25071163
  • wos:000342743500014
  • scopus:84905113844
ISSN
1540-9538
DOI
10.1084/jem.20131800
language
English
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yes
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f4e38462-9a06-4662-a0ff-cee824d1baf8 (old id 4581123)
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http://www.ncbi.nlm.nih.gov/pubmed/25071163?dopt=Abstract
date added to LUP
2014-08-09 18:06:15
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2017-11-12 03:05:48
@article{f4e38462-9a06-4662-a0ff-cee824d1baf8,
  abstract     = {Eosinophils natively inhabit the small intestine, but a functional role for them there has remained elusive. Here, we show that eosinophil-deficient mice were protected from induction of Th2-mediated peanut food allergy and anaphylaxis, and Th2 priming was restored by reconstitution with il4(+/+) or il4(-/-) eosinophils. Eosinophils controlled CD103(+) dendritic cell (DC) activation and migration from the intestine to draining lymph nodes, events necessary for Th2 priming. Eosinophil activation in vitro and in vivo led to degranulation of eosinophil peroxidase, a granule protein whose enzymatic activity promoted DC activation in mice and humans in vitro, and intestinal and extraintestinal mouse DC activation and mobilization to lymph nodes in vivo. Further, eosinophil peroxidase enhanced responses to ovalbumin seen after immunization. Thus, eosinophils can be critical contributors to the intestinal immune system, and granule-mediated shaping of DC responses can promote both intestinal and extraintestinal adaptive immunity.},
  author       = {Chu, Derek K and Jimenez-Saiz, Rodrigo and Verschoor, Christopher P and Walker, Tina D and Goncharova, Susanna and Llop-Guevara, Alba and Shen, Pamela and Gordon, Melissa E and Barra, Nicole G and Bassett, Jennifer D and Kong, Joshua and Fattouh, Ramzi and McCoy, Kathy D and Bowdish, Dawn M and Erjefält, Jonas and Pabst, Oliver and Humbles, Alison A and Kolbeck, Roland and Waserman, Susan and Jordana, Manel},
  issn         = {1540-9538},
  language     = {eng},
  number       = {8},
  pages        = {1657--1672},
  publisher    = {Rockefeller University Press},
  series       = {Journal of Experimental Medicine},
  title        = {Indigenous enteric eosinophils control DCs to initiate a primary Th2 immune response in vivo.},
  url          = {http://dx.doi.org/10.1084/jem.20131800},
  volume       = {211},
  year         = {2014},
}