Indigenous enteric eosinophils control DCs to initiate a primary Th2 immune response in vivo.
(2014) In Journal of Experimental Medicine 211(8). p.1657-1672- Abstract
- Eosinophils natively inhabit the small intestine, but a functional role for them there has remained elusive. Here, we show that eosinophil-deficient mice were protected from induction of Th2-mediated peanut food allergy and anaphylaxis, and Th2 priming was restored by reconstitution with il4(+/+) or il4(-/-) eosinophils. Eosinophils controlled CD103(+) dendritic cell (DC) activation and migration from the intestine to draining lymph nodes, events necessary for Th2 priming. Eosinophil activation in vitro and in vivo led to degranulation of eosinophil peroxidase, a granule protein whose enzymatic activity promoted DC activation in mice and humans in vitro, and intestinal and extraintestinal mouse DC activation and mobilization to lymph nodes... (More)
- Eosinophils natively inhabit the small intestine, but a functional role for them there has remained elusive. Here, we show that eosinophil-deficient mice were protected from induction of Th2-mediated peanut food allergy and anaphylaxis, and Th2 priming was restored by reconstitution with il4(+/+) or il4(-/-) eosinophils. Eosinophils controlled CD103(+) dendritic cell (DC) activation and migration from the intestine to draining lymph nodes, events necessary for Th2 priming. Eosinophil activation in vitro and in vivo led to degranulation of eosinophil peroxidase, a granule protein whose enzymatic activity promoted DC activation in mice and humans in vitro, and intestinal and extraintestinal mouse DC activation and mobilization to lymph nodes in vivo. Further, eosinophil peroxidase enhanced responses to ovalbumin seen after immunization. Thus, eosinophils can be critical contributors to the intestinal immune system, and granule-mediated shaping of DC responses can promote both intestinal and extraintestinal adaptive immunity. (Less)
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https://lup.lub.lu.se/record/4581123
- author
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Experimental Medicine
- volume
- 211
- issue
- 8
- pages
- 1657 - 1672
- publisher
- Rockefeller University Press
- external identifiers
-
- pmid:25071163
- wos:000342743500014
- scopus:84905113844
- pmid:25071163
- ISSN
- 1540-9538
- DOI
- 10.1084/jem.20131800
- language
- English
- LU publication?
- yes
- id
- f4e38462-9a06-4662-a0ff-cee824d1baf8 (old id 4581123)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25071163?dopt=Abstract
- date added to LUP
- 2016-04-01 10:15:45
- date last changed
- 2022-03-04 17:50:38
@article{f4e38462-9a06-4662-a0ff-cee824d1baf8,
abstract = {{Eosinophils natively inhabit the small intestine, but a functional role for them there has remained elusive. Here, we show that eosinophil-deficient mice were protected from induction of Th2-mediated peanut food allergy and anaphylaxis, and Th2 priming was restored by reconstitution with il4(+/+) or il4(-/-) eosinophils. Eosinophils controlled CD103(+) dendritic cell (DC) activation and migration from the intestine to draining lymph nodes, events necessary for Th2 priming. Eosinophil activation in vitro and in vivo led to degranulation of eosinophil peroxidase, a granule protein whose enzymatic activity promoted DC activation in mice and humans in vitro, and intestinal and extraintestinal mouse DC activation and mobilization to lymph nodes in vivo. Further, eosinophil peroxidase enhanced responses to ovalbumin seen after immunization. Thus, eosinophils can be critical contributors to the intestinal immune system, and granule-mediated shaping of DC responses can promote both intestinal and extraintestinal adaptive immunity.}},
author = {{Chu, Derek K and Jimenez-Saiz, Rodrigo and Verschoor, Christopher P and Walker, Tina D and Goncharova, Susanna and Llop-Guevara, Alba and Shen, Pamela and Gordon, Melissa E and Barra, Nicole G and Bassett, Jennifer D and Kong, Joshua and Fattouh, Ramzi and McCoy, Kathy D and Bowdish, Dawn M and Erjefält, Jonas and Pabst, Oliver and Humbles, Alison A and Kolbeck, Roland and Waserman, Susan and Jordana, Manel}},
issn = {{1540-9538}},
language = {{eng}},
number = {{8}},
pages = {{1657--1672}},
publisher = {{Rockefeller University Press}},
series = {{Journal of Experimental Medicine}},
title = {{Indigenous enteric eosinophils control DCs to initiate a primary Th2 immune response in vivo.}},
url = {{https://lup.lub.lu.se/search/files/1694187/5154472.pdf}},
doi = {{10.1084/jem.20131800}},
volume = {{211}},
year = {{2014}},
}