Advanced

Discovery-based Protein Expression Profiling Identifies Distinct Subgroups and Pathways in Leiomyosarcomas.

Kirik, Ufuk; Hansson, Karin; Krogh, Morten; Jönsson, Mats LU ; Nilbert, Mef LU ; James, Peter and Carneiro, Ana LU (2014) In Molecular Cancer Research 12(12). p.1729-1739
Abstract
Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathological and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human softtissue sarcomas, including subtype heterogeneity, using 2D-gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach and identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biological nodes like... (More)
Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathological and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human softtissue sarcomas, including subtype heterogeneity, using 2D-gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach and identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biological nodes like apoptosis, cytoskeleton remodeling and telomere regulation are differentially regulated among these subgroups. Finally, investigating the Kirik et al. Protein profiling of leiomyosarcoma Page 2 of 24 similarities between protein expression of leiomyosarcomas and undifferentiated pleomorphic sarcomas (UPS) revealed similar protein expression profiles for these tumors, in comparison to pleomorphic leiomyosarcomas. Implications: These results suggest that UPS tumors share a similar lineage as leiomyosarcomas, and are likely to originate from different stages of differentiation from mesenchymal stem cells to smooth muscle cells. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Cancer Research
volume
12
issue
12
pages
1729 - 1739
publisher
American Association for Cancer Research
external identifiers
  • pmid:25069693
  • wos:000346560400004
  • scopus:84919372664
ISSN
1557-3125
DOI
10.1158/1541-7786.MCR-14-0072
language
English
LU publication?
yes
id
16368bbc-e8fe-4edc-8029-9c2ced95d99e (old id 4581206)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25069693?dopt=Abstract
date added to LUP
2014-08-09 17:17:52
date last changed
2017-01-01 03:57:55
@article{16368bbc-e8fe-4edc-8029-9c2ced95d99e,
  abstract     = {Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathological and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human softtissue sarcomas, including subtype heterogeneity, using 2D-gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach and identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biological nodes like apoptosis, cytoskeleton remodeling and telomere regulation are differentially regulated among these subgroups. Finally, investigating the Kirik et al. Protein profiling of leiomyosarcoma Page 2 of 24 similarities between protein expression of leiomyosarcomas and undifferentiated pleomorphic sarcomas (UPS) revealed similar protein expression profiles for these tumors, in comparison to pleomorphic leiomyosarcomas. Implications: These results suggest that UPS tumors share a similar lineage as leiomyosarcomas, and are likely to originate from different stages of differentiation from mesenchymal stem cells to smooth muscle cells.},
  author       = {Kirik, Ufuk and Hansson, Karin and Krogh, Morten and Jönsson, Mats and Nilbert, Mef and James, Peter and Carneiro, Ana},
  issn         = {1557-3125},
  language     = {eng},
  number       = {12},
  pages        = {1729--1739},
  publisher    = {American Association for Cancer Research},
  series       = {Molecular Cancer Research},
  title        = {Discovery-based Protein Expression Profiling Identifies Distinct Subgroups and Pathways in Leiomyosarcomas.},
  url          = {http://dx.doi.org/10.1158/1541-7786.MCR-14-0072},
  volume       = {12},
  year         = {2014},
}