Discovery-based Protein Expression Profiling Identifies Distinct Subgroups and Pathways in Leiomyosarcomas.
Kirik, Ufuk ; Hansson, Karin ; Krogh, Morten ; Jönsson, Mats LU ; Nilbert, Mef LU ; James, Peter and Carneiro, Ana LU
(2014)
In Molecular Cancer Research
12(12).
p.1729-1739
- Abstract
- Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathological and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human softtissue sarcomas, including subtype heterogeneity, using 2D-gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach and identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biological nodes like... (More)
- Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathological and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human softtissue sarcomas, including subtype heterogeneity, using 2D-gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach and identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biological nodes like apoptosis, cytoskeleton remodeling and telomere regulation are differentially regulated among these subgroups. Finally, investigating the Kirik et al. Protein profiling of leiomyosarcoma Page 2 of 24 similarities between protein expression of leiomyosarcomas and undifferentiated pleomorphic sarcomas (UPS) revealed similar protein expression profiles for these tumors, in comparison to pleomorphic leiomyosarcomas. Implications: These results suggest that UPS tumors share a similar lineage as leiomyosarcomas, and are likely to originate from different stages of differentiation from mesenchymal stem cells to smooth muscle cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4581206
- author
- Kirik, Ufuk
; Hansson, Karin
; Krogh, Morten
; Jönsson, Mats
LU
; Nilbert, Mef
LU
; James, Peter
and Carneiro, Ana
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Cancer Research
- volume
- 12
- issue
- 12
- pages
- 1729 - 1739
- publisher
- American Association for Cancer Research
- external identifiers
-
- pmid:25069693
- wos:000346560400004
- scopus:84919372664
- pmid:25069693
- ISSN
- 1557-3125
- DOI
- 10.1158/1541-7786.MCR-14-0072
- project
- Precision Medicine in Hereditary Cancer and Sarcoma; targeted surveillance, immunotherapy and individualized follow-up
- language
- English
- LU publication?
- yes
- id
- 16368bbc-e8fe-4edc-8029-9c2ced95d99e (old id 4581206)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25069693?dopt=Abstract
- date added to LUP
- 2016-04-01 10:51:59
- date last changed
- 2022-01-26 03:12:10
@article{16368bbc-e8fe-4edc-8029-9c2ced95d99e,
abstract = {{Soft tissue sarcomas (STS) are malignant tumors of mesenchymal origin. A substantial portion of these tumors exhibits complex karyotypes and lack characterized chromosomal aberrations. Owing to such properties, both histopathological and molecular classification of these tumors has been a significant challenge. This study examines the protein expression of a large number of human softtissue sarcomas, including subtype heterogeneity, using 2D-gel proteomics. In addition, detailed proteome profiles of a subset of pleomorphic STS specimens using an in-depth mass-spectrometry approach and identified subgroups within the leiomyosarcomas with distinct protein expression patterns. Pathways analysis indicates that key biological nodes like apoptosis, cytoskeleton remodeling and telomere regulation are differentially regulated among these subgroups. Finally, investigating the Kirik et al. Protein profiling of leiomyosarcoma Page 2 of 24 similarities between protein expression of leiomyosarcomas and undifferentiated pleomorphic sarcomas (UPS) revealed similar protein expression profiles for these tumors, in comparison to pleomorphic leiomyosarcomas. Implications: These results suggest that UPS tumors share a similar lineage as leiomyosarcomas, and are likely to originate from different stages of differentiation from mesenchymal stem cells to smooth muscle cells.}},
author = {{Kirik, Ufuk and Hansson, Karin and Krogh, Morten and Jönsson, Mats and Nilbert, Mef and James, Peter and Carneiro, Ana}},
issn = {{1557-3125}},
language = {{eng}},
number = {{12}},
pages = {{1729--1739}},
publisher = {{American Association for Cancer Research}},
series = {{Molecular Cancer Research}},
title = {{Discovery-based Protein Expression Profiling Identifies Distinct Subgroups and Pathways in Leiomyosarcomas.}},
url = {{http://dx.doi.org/10.1158/1541-7786.MCR-14-0072}},
doi = {{10.1158/1541-7786.MCR-14-0072}},
volume = {{12}},
year = {{2014}},
}