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A novel multi-strain probiotic and synbiotic supplement for prevention of Clostridium difficile infection in a murine model.

Kondepudi, Kanthi Kiran; Ambalam, Padma; Karagin, Peren H; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU (2014) In Microbiology and Immunology 58(10). p.552-558
Abstract
The protective effect of a multi-strain probiotic and synbiotic formulation was evaluated in C57BL/6 mice infected with Clostridium difficile (CD) NAP1/027. Antibiotic treated mice were divided into four groups. Group 1, fed with a synbiotic formulation consisting of Lactobacillus plantarum F44, L. paracasei F8, Bifidobacterium breve 46, B. lactis 8:8, galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMOS) and resistant starch (RS); group 2, fed with the same four probiotic strains as in group 1; group 3, fed with the same prebiotic supplements as mentioned in group 1 for seven days before CD infection and group 4, the control group, was antibiotic treated and infected with NAP1/027 strain. Faeces and caecal contents were... (More)
The protective effect of a multi-strain probiotic and synbiotic formulation was evaluated in C57BL/6 mice infected with Clostridium difficile (CD) NAP1/027. Antibiotic treated mice were divided into four groups. Group 1, fed with a synbiotic formulation consisting of Lactobacillus plantarum F44, L. paracasei F8, Bifidobacterium breve 46, B. lactis 8:8, galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMOS) and resistant starch (RS); group 2, fed with the same four probiotic strains as in group 1; group 3, fed with the same prebiotic supplements as mentioned in group 1 for seven days before CD infection and group 4, the control group, was antibiotic treated and infected with NAP1/027 strain. Faeces and caecal contents were collected for microbial cell viability, quantitative PCR (qPCR), toxin analyses and histopathology. Synbiotics and probiotics fed mice showed a significant increase of total bifidobacteria (P < 0.05). Total lactobacilli count was increased in group 1. The caecal toxins were negative in group 2 mice, and one sample each from group 1 and 3 was positive. qPCR of caecal content showed significant reduction in NAP1/027 DNA copies in group 1-2 and significantly higher numbers of B. breve 46, L. plantarum F44 and L. paracasei F8 in group 1 and 2 (P < 0.05) but much less pronounced in group 3-4. This study demonstrated that the newly developed synbiotic or multi-strain probiotic formulation conferred protection against NAP1/027 infection in C57BL/6 mice. This holds promising to conduct future human studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbiology and Immunology
volume
58
issue
10
pages
552 - 558
publisher
Center for Academic Publications Japan
external identifiers
  • pmid:25059277
  • wos:000342912900002
  • scopus:84908874020
ISSN
1348-0421
DOI
10.1111/1348-0421.12184
language
English
LU publication?
yes
id
d997918a-94ae-446d-bc39-9aede036f472 (old id 4581359)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25059277?dopt=Abstract
date added to LUP
2014-08-09 15:23:15
date last changed
2017-04-30 03:18:45
@article{d997918a-94ae-446d-bc39-9aede036f472,
  abstract     = {The protective effect of a multi-strain probiotic and synbiotic formulation was evaluated in C57BL/6 mice infected with Clostridium difficile (CD) NAP1/027. Antibiotic treated mice were divided into four groups. Group 1, fed with a synbiotic formulation consisting of Lactobacillus plantarum F44, L. paracasei F8, Bifidobacterium breve 46, B. lactis 8:8, galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMOS) and resistant starch (RS); group 2, fed with the same four probiotic strains as in group 1; group 3, fed with the same prebiotic supplements as mentioned in group 1 for seven days before CD infection and group 4, the control group, was antibiotic treated and infected with NAP1/027 strain. Faeces and caecal contents were collected for microbial cell viability, quantitative PCR (qPCR), toxin analyses and histopathology. Synbiotics and probiotics fed mice showed a significant increase of total bifidobacteria (P &lt; 0.05). Total lactobacilli count was increased in group 1. The caecal toxins were negative in group 2 mice, and one sample each from group 1 and 3 was positive. qPCR of caecal content showed significant reduction in NAP1/027 DNA copies in group 1-2 and significantly higher numbers of B. breve 46, L. plantarum F44 and L. paracasei F8 in group 1 and 2 (P &lt; 0.05) but much less pronounced in group 3-4. This study demonstrated that the newly developed synbiotic or multi-strain probiotic formulation conferred protection against NAP1/027 infection in C57BL/6 mice. This holds promising to conduct future human studies.},
  author       = {Kondepudi, Kanthi Kiran and Ambalam, Padma and Karagin, Peren H and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa},
  issn         = {1348-0421},
  language     = {eng},
  number       = {10},
  pages        = {552--558},
  publisher    = {Center for Academic Publications Japan},
  series       = {Microbiology and Immunology},
  title        = {A novel multi-strain probiotic and synbiotic supplement for prevention of Clostridium difficile infection in a murine model.},
  url          = {http://dx.doi.org/10.1111/1348-0421.12184},
  volume       = {58},
  year         = {2014},
}