A novel multi-strain probiotic and synbiotic supplement for prevention of Clostridium difficile infection in a murine model.
(2014) In Microbiology and Immunology 58(10). p.552-558- Abstract
- The protective effect of a multi-strain probiotic and synbiotic formulation was evaluated in C57BL/6 mice infected with Clostridium difficile (CD) NAP1/027. Antibiotic treated mice were divided into four groups. Group 1, fed with a synbiotic formulation consisting of Lactobacillus plantarum F44, L. paracasei F8, Bifidobacterium breve 46, B. lactis 8:8, galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMOS) and resistant starch (RS); group 2, fed with the same four probiotic strains as in group 1; group 3, fed with the same prebiotic supplements as mentioned in group 1 for seven days before CD infection and group 4, the control group, was antibiotic treated and infected with NAP1/027 strain. Faeces and caecal contents were... (More)
- The protective effect of a multi-strain probiotic and synbiotic formulation was evaluated in C57BL/6 mice infected with Clostridium difficile (CD) NAP1/027. Antibiotic treated mice were divided into four groups. Group 1, fed with a synbiotic formulation consisting of Lactobacillus plantarum F44, L. paracasei F8, Bifidobacterium breve 46, B. lactis 8:8, galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMOS) and resistant starch (RS); group 2, fed with the same four probiotic strains as in group 1; group 3, fed with the same prebiotic supplements as mentioned in group 1 for seven days before CD infection and group 4, the control group, was antibiotic treated and infected with NAP1/027 strain. Faeces and caecal contents were collected for microbial cell viability, quantitative PCR (qPCR), toxin analyses and histopathology. Synbiotics and probiotics fed mice showed a significant increase of total bifidobacteria (P < 0.05). Total lactobacilli count was increased in group 1. The caecal toxins were negative in group 2 mice, and one sample each from group 1 and 3 was positive. qPCR of caecal content showed significant reduction in NAP1/027 DNA copies in group 1-2 and significantly higher numbers of B. breve 46, L. plantarum F44 and L. paracasei F8 in group 1 and 2 (P < 0.05) but much less pronounced in group 3-4. This study demonstrated that the newly developed synbiotic or multi-strain probiotic formulation conferred protection against NAP1/027 infection in C57BL/6 mice. This holds promising to conduct future human studies. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4581359
- author
- Kondepudi, Kanthi Kiran ; Ambalam, Padma ; Karagin, Peren H ; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microbiology and Immunology
- volume
- 58
- issue
- 10
- pages
- 552 - 558
- publisher
- Center for Academic Publications Japan
- external identifiers
-
- pmid:25059277
- wos:000342912900002
- scopus:84908874020
- pmid:25059277
- ISSN
- 1348-0421
- DOI
- 10.1111/1348-0421.12184
- language
- English
- LU publication?
- yes
- id
- d997918a-94ae-446d-bc39-9aede036f472 (old id 4581359)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25059277?dopt=Abstract
- date added to LUP
- 2016-04-01 09:53:09
- date last changed
- 2022-02-02 03:49:09
@article{d997918a-94ae-446d-bc39-9aede036f472, abstract = {{The protective effect of a multi-strain probiotic and synbiotic formulation was evaluated in C57BL/6 mice infected with Clostridium difficile (CD) NAP1/027. Antibiotic treated mice were divided into four groups. Group 1, fed with a synbiotic formulation consisting of Lactobacillus plantarum F44, L. paracasei F8, Bifidobacterium breve 46, B. lactis 8:8, galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMOS) and resistant starch (RS); group 2, fed with the same four probiotic strains as in group 1; group 3, fed with the same prebiotic supplements as mentioned in group 1 for seven days before CD infection and group 4, the control group, was antibiotic treated and infected with NAP1/027 strain. Faeces and caecal contents were collected for microbial cell viability, quantitative PCR (qPCR), toxin analyses and histopathology. Synbiotics and probiotics fed mice showed a significant increase of total bifidobacteria (P < 0.05). Total lactobacilli count was increased in group 1. The caecal toxins were negative in group 2 mice, and one sample each from group 1 and 3 was positive. qPCR of caecal content showed significant reduction in NAP1/027 DNA copies in group 1-2 and significantly higher numbers of B. breve 46, L. plantarum F44 and L. paracasei F8 in group 1 and 2 (P < 0.05) but much less pronounced in group 3-4. This study demonstrated that the newly developed synbiotic or multi-strain probiotic formulation conferred protection against NAP1/027 infection in C57BL/6 mice. This holds promising to conduct future human studies.}}, author = {{Kondepudi, Kanthi Kiran and Ambalam, Padma and Karagin, Peren H and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa}}, issn = {{1348-0421}}, language = {{eng}}, number = {{10}}, pages = {{552--558}}, publisher = {{Center for Academic Publications Japan}}, series = {{Microbiology and Immunology}}, title = {{A novel multi-strain probiotic and synbiotic supplement for prevention of Clostridium difficile infection in a murine model.}}, url = {{http://dx.doi.org/10.1111/1348-0421.12184}}, doi = {{10.1111/1348-0421.12184}}, volume = {{58}}, year = {{2014}}, }