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Size-Dependent Effects of Nanoparticles on Enzymes in the Blood Coagulation Cascade.

Sanfins, Elodie LU ; Augustsson, Cecilia; Dahlbäck, Björn LU ; Linse, Sara and Cedervall, Tommy (2014) In Nano Letters 14(8). p.4736-4744
Abstract
Nanoparticles (NPs) are increasingly used in diagnostic and drug delivery. After entering the bloodstream, a protein corona will form around NPs. The size and curvature of NPs is one of the major characteristics affecting the composition of bound protein in the corona. Key initiators of the intrinsic pathway of blood coagulation, the contact activation complex, (Kallikrein, Factor XII, and high molecular weight Kininogen) have previously been identified on NPs surfaces. We show that the functional impact of carboxyl-modified polystyrene NPs on these initiators of the intrinsic pathway is size dependent. NPs with high curvature affect the enzymatic activity differently from NPs with low curvature. The size dependency is evident in full... (More)
Nanoparticles (NPs) are increasingly used in diagnostic and drug delivery. After entering the bloodstream, a protein corona will form around NPs. The size and curvature of NPs is one of the major characteristics affecting the composition of bound protein in the corona. Key initiators of the intrinsic pathway of blood coagulation, the contact activation complex, (Kallikrein, Factor XII, and high molecular weight Kininogen) have previously been identified on NPs surfaces. We show that the functional impact of carboxyl-modified polystyrene NPs on these initiators of the intrinsic pathway is size dependent. NPs with high curvature affect the enzymatic activity differently from NPs with low curvature. The size dependency is evident in full blood plasma as well as in solutions of single coagulation factors. NPs induce significant alteration of the enzymatic activity in a size-dependent manner, and enzyme kinetics studies show a critical role for NPs surface area and curvature. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nano Letters
volume
14
issue
8
pages
4736 - 4744
publisher
The American Chemical Society
external identifiers
  • pmid:25025946
  • wos:000340446200083
  • scopus:84906094819
ISSN
1530-6992
DOI
10.1021/nl501863u
language
English
LU publication?
yes
id
691271d2-5d04-4edf-bede-8f7e651073eb (old id 4582238)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25025946?dopt=Abstract
date added to LUP
2014-08-08 00:14:21
date last changed
2017-11-19 03:20:37
@article{691271d2-5d04-4edf-bede-8f7e651073eb,
  abstract     = {Nanoparticles (NPs) are increasingly used in diagnostic and drug delivery. After entering the bloodstream, a protein corona will form around NPs. The size and curvature of NPs is one of the major characteristics affecting the composition of bound protein in the corona. Key initiators of the intrinsic pathway of blood coagulation, the contact activation complex, (Kallikrein, Factor XII, and high molecular weight Kininogen) have previously been identified on NPs surfaces. We show that the functional impact of carboxyl-modified polystyrene NPs on these initiators of the intrinsic pathway is size dependent. NPs with high curvature affect the enzymatic activity differently from NPs with low curvature. The size dependency is evident in full blood plasma as well as in solutions of single coagulation factors. NPs induce significant alteration of the enzymatic activity in a size-dependent manner, and enzyme kinetics studies show a critical role for NPs surface area and curvature.},
  author       = {Sanfins, Elodie and Augustsson, Cecilia and Dahlbäck, Björn and Linse, Sara and Cedervall, Tommy},
  issn         = {1530-6992},
  language     = {eng},
  number       = {8},
  pages        = {4736--4744},
  publisher    = {The American Chemical Society},
  series       = {Nano Letters},
  title        = {Size-Dependent Effects of Nanoparticles on Enzymes in the Blood Coagulation Cascade.},
  url          = {http://dx.doi.org/10.1021/nl501863u},
  volume       = {14},
  year         = {2014},
}