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Inactivation and Adsorption of Human Carbonic Anhydrase II by Nanoparticles.

Assarsson, Anna LU ; Pastoriza-Santos, Isabel and Cabaleiro-Lago, Celia LU (2014) In Langmuir 30(31). p.9448-9456
Abstract
The enzymatic activity of human carbonic anhydrase II (HCAII) was studied in the presence of nanoparticles of different nature and charge. Negatively charged nanoparticles inhibit HCAII whereas no effect is seen for positively charged particles. The kinetic effects were correlated with the strength of binding of the enzyme to the particle surface as measured by ITC and adsorption assays. Moreover, conformational changes upon adsorption were observed by circular dichroism. The main initial driving force for the adsorption of HCAII to nanoparticles is of electrostatic nature whereas the hydrophobic effect is not strong enough to drive the initial binding. This is corroborated by the fact that HCAII do not adsorb on positively charged... (More)
The enzymatic activity of human carbonic anhydrase II (HCAII) was studied in the presence of nanoparticles of different nature and charge. Negatively charged nanoparticles inhibit HCAII whereas no effect is seen for positively charged particles. The kinetic effects were correlated with the strength of binding of the enzyme to the particle surface as measured by ITC and adsorption assays. Moreover, conformational changes upon adsorption were observed by circular dichroism. The main initial driving force for the adsorption of HCAII to nanoparticles is of electrostatic nature whereas the hydrophobic effect is not strong enough to drive the initial binding. This is corroborated by the fact that HCAII do not adsorb on positively charged hydrophobic polystyrene nanoparticles. Furthermore, the dehydration of the particle and protein surface seems to play an important role in the inactivation of HCAII by carboxyl-modified polystyrene nanoparticles. On the other hand, the inactivation by unmodified polystyrene nanoparticles is mainly driven by intramolecular interactions established between the protein and the nanoparticle surface upon conformational changes in the protein. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
30
issue
31
pages
9448 - 9456
publisher
The American Chemical Society
external identifiers
  • pmid:24999988
  • wos:000340347300026
  • scopus:84905990191
ISSN
0743-7463
DOI
10.1021/la501413r
language
English
LU publication?
yes
id
31c90886-f21f-42ef-9cb2-ee52a5769ce4 (old id 4583315)
date added to LUP
2014-09-12 16:48:34
date last changed
2017-07-02 03:02:06
@article{31c90886-f21f-42ef-9cb2-ee52a5769ce4,
  abstract     = {The enzymatic activity of human carbonic anhydrase II (HCAII) was studied in the presence of nanoparticles of different nature and charge. Negatively charged nanoparticles inhibit HCAII whereas no effect is seen for positively charged particles. The kinetic effects were correlated with the strength of binding of the enzyme to the particle surface as measured by ITC and adsorption assays. Moreover, conformational changes upon adsorption were observed by circular dichroism. The main initial driving force for the adsorption of HCAII to nanoparticles is of electrostatic nature whereas the hydrophobic effect is not strong enough to drive the initial binding. This is corroborated by the fact that HCAII do not adsorb on positively charged hydrophobic polystyrene nanoparticles. Furthermore, the dehydration of the particle and protein surface seems to play an important role in the inactivation of HCAII by carboxyl-modified polystyrene nanoparticles. On the other hand, the inactivation by unmodified polystyrene nanoparticles is mainly driven by intramolecular interactions established between the protein and the nanoparticle surface upon conformational changes in the protein.},
  author       = {Assarsson, Anna and Pastoriza-Santos, Isabel and Cabaleiro-Lago, Celia},
  issn         = {0743-7463},
  language     = {eng},
  number       = {31},
  pages        = {9448--9456},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Inactivation and Adsorption of Human Carbonic Anhydrase II by Nanoparticles.},
  url          = {http://dx.doi.org/10.1021/la501413r},
  volume       = {30},
  year         = {2014},
}