Gas phase silylation, a rapid method for preparation of high-performance liquid chromatography supports
(1988) In Journal of chromatography. A 455. p.105-117- Abstract
- A new method for preparing chromatographic supports is described. Porous silica was treated with gaseous silanes and gaseous triethylamine at high temperature and reduced pressure. The silylation procedure was rapid and gave supports with a covalently bound monolayer of aminopropyl-or epoxysilane. Diol silica prepared by gas phase silylation was compared with diol silica prepared in the aqueous or organic phase (toluene). Seven proteins were chromatographed and the protein recovery, plate number and peak asymmetry were calculated. Silica prepared by the gas phase silylation method showed improved performance. The separation of carbonhydrates on aminopropylsilica and the isolation of lactate dehydrogenase with nicotinamide-adenine... (More)
- A new method for preparing chromatographic supports is described. Porous silica was treated with gaseous silanes and gaseous triethylamine at high temperature and reduced pressure. The silylation procedure was rapid and gave supports with a covalently bound monolayer of aminopropyl-or epoxysilane. Diol silica prepared by gas phase silylation was compared with diol silica prepared in the aqueous or organic phase (toluene). Seven proteins were chromatographed and the protein recovery, plate number and peak asymmetry were calculated. Silica prepared by the gas phase silylation method showed improved performance. The separation of carbonhydrates on aminopropylsilica and the isolation of lactate dehydrogenase with nicotinamide-adenine dinucleotide-silica, prepared from gas phase silylated diol silica, are described. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4590d742-f343-423b-a447-4dcce167f8ef
- author
- Wikström, Per ; Mandenius, Carl Fredrik and Larsson, Per-Olof LU
- organization
- publishing date
- 1988
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gas phase silylation, Silica, HPLC supports
- in
- Journal of chromatography. A
- volume
- 455
- pages
- 13 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0001022722
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(01)82110-5
- language
- English
- LU publication?
- yes
- id
- 4590d742-f343-423b-a447-4dcce167f8ef
- date added to LUP
- 2024-06-18 13:54:18
- date last changed
- 2025-04-04 14:42:57
@article{4590d742-f343-423b-a447-4dcce167f8ef,
abstract = {{A new method for preparing chromatographic supports is described. Porous silica was treated with gaseous silanes and gaseous triethylamine at high temperature and reduced pressure. The silylation procedure was rapid and gave supports with a covalently bound monolayer of aminopropyl-or epoxysilane. Diol silica prepared by gas phase silylation was compared with diol silica prepared in the aqueous or organic phase (toluene). Seven proteins were chromatographed and the protein recovery, plate number and peak asymmetry were calculated. Silica prepared by the gas phase silylation method showed improved performance. The separation of carbonhydrates on aminopropylsilica and the isolation of lactate dehydrogenase with nicotinamide-adenine dinucleotide-silica, prepared from gas phase silylated diol silica, are described.}},
author = {{Wikström, Per and Mandenius, Carl Fredrik and Larsson, Per-Olof}},
issn = {{0021-9673}},
keywords = {{Gas phase silylation; Silica; HPLC supports}},
language = {{eng}},
pages = {{105--117}},
publisher = {{Elsevier}},
series = {{Journal of chromatography. A}},
title = {{Gas phase silylation, a rapid method for preparation of high-performance liquid chromatography supports}},
url = {{http://dx.doi.org/10.1016/S0021-9673(01)82110-5}},
doi = {{10.1016/S0021-9673(01)82110-5}},
volume = {{455}},
year = {{1988}},
}